Strain Fitness in Escherichia coli ECOR38 around HEPCGN_14525

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaJ and HEPCGN_14525 are separated by 78 nucleotidesHEPCGN_14525 and HEPCGN_14530 are separated by 45 nucleotides HEPCGN_14520: rfaJ - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 1,656,991 to 1,658,019 rfaJ HEPCGN_14525: HEPCGN_14525 - beta1,3-glucosyltransferase, at 1,658,098 to 1,659,081 _14525 HEPCGN_14530: HEPCGN_14530 - O-antigen ligase domain-containing protein, at 1,659,127 to 1,660,380 _14530 Position (kb) 1658 1659 1660Strain fitness (log2 ratio) -3 -2 -1 0 1at 1657.113 kb on + strand, within rfaJat 1657.114 kb on - strand, within rfaJat 1657.213 kb on - strand, within rfaJat 1657.363 kb on + strand, within rfaJat 1657.852 kb on + strand, within rfaJat 1658.233 kb on - strand, within HEPCGN_14525at 1658.338 kb on - strand, within HEPCGN_14525at 1658.363 kb on + strand, within HEPCGN_14525at 1658.452 kb on + strand, within HEPCGN_14525at 1658.476 kb on - strand, within HEPCGN_14525at 1658.477 kb on + strand, within HEPCGN_14525at 1658.504 kb on + strand, within HEPCGN_14525at 1658.673 kb on - strand, within HEPCGN_14525at 1658.719 kb on - strand, within HEPCGN_14525at 1658.800 kb on + strand, within HEPCGN_14525at 1659.014 kb on + strandat 1659.108 kb on + strandat 1659.182 kb on + strandat 1659.183 kb on - strandat 1659.444 kb on + strand, within HEPCGN_14530at 1659.461 kb on + strand, within HEPCGN_14530at 1659.732 kb on - strand, within HEPCGN_14530at 1659.744 kb on - strand, within HEPCGN_14530at 1660.078 kb on - strand, within HEPCGN_14530

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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1,657,113 + rfaJ HEPCGN_14520 0.12 -2.2
1,657,114 - rfaJ HEPCGN_14520 0.12 -1.5
1,657,213 - rfaJ HEPCGN_14520 0.22 -1.0
1,657,363 + rfaJ HEPCGN_14520 0.36 -0.5
1,657,852 + rfaJ HEPCGN_14520 0.84 +0.8
1,658,233 - HEPCGN_14525 0.14 +0.1
1,658,338 - HEPCGN_14525 0.24 -2.0
1,658,363 + HEPCGN_14525 0.27 -1.4
1,658,452 + HEPCGN_14525 0.36 -2.2
1,658,476 - HEPCGN_14525 0.38 +0.1
1,658,477 + HEPCGN_14525 0.39 -0.7
1,658,504 + HEPCGN_14525 0.41 -2.7
1,658,673 - HEPCGN_14525 0.58 -1.3
1,658,719 - HEPCGN_14525 0.63 -2.2
1,658,800 + HEPCGN_14525 0.71 -0.7
1,659,014 + -3.1
1,659,108 + -0.2
1,659,182 + +1.0
1,659,183 - -1.5
1,659,444 + HEPCGN_14530 0.25 -0.6
1,659,461 + HEPCGN_14530 0.27 -2.2
1,659,732 - HEPCGN_14530 0.48 -0.5
1,659,744 - HEPCGN_14530 0.49 +1.5
1,660,078 - HEPCGN_14530 0.76 -0.0

Or see this region's nucleotide sequence