Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaQ and rfaG overlap by 4 nucleotides rfaG and rfaP overlap by 8 nucleotides rfaP and waaO are separated by 15 nucleotides
HEPCGN_14475: rfaQ - lipopolysaccharide core heptosyltransferase RfaQ, at 1,649,912 to 1,650,970
rfaQ
HEPCGN_14480: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 1,650,967 to 1,652,091
rfaG
HEPCGN_14485: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 1,652,084 to 1,652,881
rfaP
HEPCGN_14490: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,652,897 to 1,653,913
waaO
Position (kb) 1650
1651
1652
1653 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1649.970 kb on + strand at 1649.970 kb on + strand at 1649.977 kb on + strand at 1649.977 kb on + strand at 1649.989 kb on + strand at 1650.156 kb on + strand, within rfaQ at 1650.157 kb on - strand, within rfaQ at 1650.157 kb on - strand, within rfaQ at 1650.159 kb on + strand, within rfaQ at 1650.159 kb on + strand, within rfaQ at 1650.160 kb on - strand, within rfaQ at 1650.169 kb on + strand, within rfaQ at 1650.181 kb on + strand, within rfaQ at 1650.214 kb on + strand, within rfaQ at 1650.214 kb on + strand, within rfaQ at 1650.215 kb on - strand, within rfaQ at 1650.215 kb on - strand, within rfaQ at 1650.216 kb on + strand, within rfaQ at 1650.231 kb on + strand, within rfaQ at 1650.260 kb on + strand, within rfaQ at 1650.292 kb on + strand, within rfaQ at 1650.340 kb on + strand, within rfaQ at 1650.341 kb on - strand, within rfaQ at 1650.543 kb on + strand, within rfaQ at 1650.544 kb on - strand, within rfaQ at 1650.544 kb on - strand, within rfaQ at 1650.544 kb on - strand, within rfaQ at 1650.544 kb on - strand, within rfaQ at 1650.586 kb on + strand, within rfaQ at 1650.642 kb on + strand, within rfaQ at 1650.737 kb on - strand, within rfaQ at 1650.751 kb on + strand, within rfaQ at 1650.755 kb on - strand, within rfaQ at 1650.888 kb on - strand at 1650.987 kb on - strand at 1650.990 kb on + strand at 1650.990 kb on + strand at 1650.991 kb on - strand at 1650.994 kb on + strand at 1651.109 kb on + strand, within rfaG at 1651.134 kb on + strand, within rfaG at 1651.143 kb on + strand, within rfaG at 1651.144 kb on - strand, within rfaG at 1651.168 kb on + strand, within rfaG at 1651.169 kb on - strand, within rfaG at 1651.252 kb on + strand, within rfaG at 1651.255 kb on + strand, within rfaG at 1651.273 kb on + strand, within rfaG at 1651.336 kb on + strand, within rfaG at 1651.337 kb on - strand, within rfaG at 1651.485 kb on + strand, within rfaG at 1651.486 kb on - strand, within rfaG at 1651.489 kb on + strand, within rfaG at 1651.541 kb on + strand, within rfaG at 1651.555 kb on + strand, within rfaG at 1651.556 kb on - strand, within rfaG at 1651.556 kb on - strand, within rfaG at 1651.590 kb on + strand, within rfaG at 1651.658 kb on + strand, within rfaG at 1651.658 kb on + strand, within rfaG at 1651.658 kb on + strand, within rfaG at 1651.659 kb on - strand, within rfaG at 1651.659 kb on - strand, within rfaG at 1651.659 kb on - strand, within rfaG at 1651.659 kb on - strand, within rfaG at 1651.660 kb on + strand, within rfaG at 1651.661 kb on - strand, within rfaG at 1651.726 kb on - strand, within rfaG at 1651.784 kb on + strand, within rfaG at 1651.787 kb on + strand, within rfaG at 1651.839 kb on + strand, within rfaG at 1651.859 kb on + strand, within rfaG at 1651.859 kb on + strand, within rfaG at 1651.859 kb on + strand, within rfaG at 1651.859 kb on + strand, within rfaG at 1651.859 kb on + strand, within rfaG at 1651.888 kb on + strand, within rfaG at 1651.889 kb on - strand, within rfaG at 1652.026 kb on - strand at 1652.036 kb on + strand at 1652.037 kb on - strand at 1652.126 kb on - strand at 1652.262 kb on + strand, within rfaP at 1652.262 kb on + strand, within rfaP at 1652.354 kb on - strand, within rfaP at 1652.356 kb on + strand, within rfaP at 1652.356 kb on + strand, within rfaP at 1652.356 kb on + strand, within rfaP at 1652.356 kb on + strand, within rfaP at 1652.357 kb on - strand, within rfaP at 1652.360 kb on + strand, within rfaP at 1652.361 kb on - strand, within rfaP at 1652.361 kb on - strand, within rfaP at 1652.457 kb on - strand, within rfaP at 1652.503 kb on + strand, within rfaP at 1652.512 kb on + strand, within rfaP at 1652.574 kb on - strand, within rfaP at 1652.574 kb on - strand, within rfaP at 1652.576 kb on - strand, within rfaP at 1652.701 kb on + strand, within rfaP at 1652.761 kb on + strand, within rfaP at 1652.771 kb on + strand, within rfaP at 1653.058 kb on + strand, within waaO
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,649,970 + -0.6 1,649,970 + +1.2 1,649,977 + -1.6 1,649,977 + +1.0 1,649,989 + +0.9 1,650,156 + rfaQ HEPCGN_14475 0.23 -0.6 1,650,157 - rfaQ HEPCGN_14475 0.23 -0.7 1,650,157 - rfaQ HEPCGN_14475 0.23 -1.2 1,650,159 + rfaQ HEPCGN_14475 0.23 +0.9 1,650,159 + rfaQ HEPCGN_14475 0.23 +0.2 1,650,160 - rfaQ HEPCGN_14475 0.23 -1.9 1,650,169 + rfaQ HEPCGN_14475 0.24 -0.8 1,650,181 + rfaQ HEPCGN_14475 0.25 +0.8 1,650,214 + rfaQ HEPCGN_14475 0.29 -0.3 1,650,214 + rfaQ HEPCGN_14475 0.29 -0.4 1,650,215 - rfaQ HEPCGN_14475 0.29 +1.0 1,650,215 - rfaQ HEPCGN_14475 0.29 -1.9 1,650,216 + rfaQ HEPCGN_14475 0.29 +0.7 1,650,231 + rfaQ HEPCGN_14475 0.30 -1.0 1,650,260 + rfaQ HEPCGN_14475 0.33 +0.8 1,650,292 + rfaQ HEPCGN_14475 0.36 -0.4 1,650,340 + rfaQ HEPCGN_14475 0.40 -0.3 1,650,341 - rfaQ HEPCGN_14475 0.41 -0.9 1,650,543 + rfaQ HEPCGN_14475 0.60 +0.8 1,650,544 - rfaQ HEPCGN_14475 0.60 -0.4 1,650,544 - rfaQ HEPCGN_14475 0.60 +1.5 1,650,544 - rfaQ HEPCGN_14475 0.60 -0.4 1,650,544 - rfaQ HEPCGN_14475 0.60 -1.0 1,650,586 + rfaQ HEPCGN_14475 0.64 -0.7 1,650,642 + rfaQ HEPCGN_14475 0.69 -2.1 1,650,737 - rfaQ HEPCGN_14475 0.78 -1.4 1,650,751 + rfaQ HEPCGN_14475 0.79 +0.3 1,650,755 - rfaQ HEPCGN_14475 0.80 -0.5 1,650,888 - -0.7 1,650,987 - -0.7 1,650,990 + -1.3 1,650,990 + -0.0 1,650,991 - -0.9 1,650,994 + -0.7 1,651,109 + rfaG HEPCGN_14480 0.13 +0.6 1,651,134 + rfaG HEPCGN_14480 0.15 -1.4 1,651,143 + rfaG HEPCGN_14480 0.16 +0.6 1,651,144 - rfaG HEPCGN_14480 0.16 -2.3 1,651,168 + rfaG HEPCGN_14480 0.18 -1.4 1,651,169 - rfaG HEPCGN_14480 0.18 -0.1 1,651,252 + rfaG HEPCGN_14480 0.25 -1.8 1,651,255 + rfaG HEPCGN_14480 0.26 +0.6 1,651,273 + rfaG HEPCGN_14480 0.27 +0.6 1,651,336 + rfaG HEPCGN_14480 0.33 +0.3 1,651,337 - rfaG HEPCGN_14480 0.33 -2.9 1,651,485 + rfaG HEPCGN_14480 0.46 -0.9 1,651,486 - rfaG HEPCGN_14480 0.46 -0.3 1,651,489 + rfaG HEPCGN_14480 0.46 +1.9 1,651,541 + rfaG HEPCGN_14480 0.51 +0.9 1,651,555 + rfaG HEPCGN_14480 0.52 -0.9 1,651,556 - rfaG HEPCGN_14480 0.52 -0.3 1,651,556 - rfaG HEPCGN_14480 0.52 -2.5 1,651,590 + rfaG HEPCGN_14480 0.55 -0.9 1,651,658 + rfaG HEPCGN_14480 0.61 -1.7 1,651,658 + rfaG HEPCGN_14480 0.61 -1.4 1,651,658 + rfaG HEPCGN_14480 0.61 +1.5 1,651,659 - rfaG HEPCGN_14480 0.62 -0.1 1,651,659 - rfaG HEPCGN_14480 0.62 -0.2 1,651,659 - rfaG HEPCGN_14480 0.62 +0.8 1,651,659 - rfaG HEPCGN_14480 0.62 -1.5 1,651,660 + rfaG HEPCGN_14480 0.62 +0.1 1,651,661 - rfaG HEPCGN_14480 0.62 +1.8 1,651,726 - rfaG HEPCGN_14480 0.67 -2.5 1,651,784 + rfaG HEPCGN_14480 0.73 -1.0 1,651,787 + rfaG HEPCGN_14480 0.73 -1.2 1,651,839 + rfaG HEPCGN_14480 0.78 -0.3 1,651,859 + rfaG HEPCGN_14480 0.79 +0.7 1,651,859 + rfaG HEPCGN_14480 0.79 -1.5 1,651,859 + rfaG HEPCGN_14480 0.79 -0.9 1,651,859 + rfaG HEPCGN_14480 0.79 -2.1 1,651,859 + rfaG HEPCGN_14480 0.79 +1.0 1,651,888 + rfaG HEPCGN_14480 0.82 -1.3 1,651,889 - rfaG HEPCGN_14480 0.82 -0.8 1,652,026 - -0.8 1,652,036 + +1.1 1,652,037 - -0.0 1,652,126 - -1.3 1,652,262 + rfaP HEPCGN_14485 0.22 -0.4 1,652,262 + rfaP HEPCGN_14485 0.22 +0.7 1,652,354 - rfaP HEPCGN_14485 0.34 -0.5 1,652,356 + rfaP HEPCGN_14485 0.34 -2.0 1,652,356 + rfaP HEPCGN_14485 0.34 -0.1 1,652,356 + rfaP HEPCGN_14485 0.34 -2.2 1,652,356 + rfaP HEPCGN_14485 0.34 -0.7 1,652,357 - rfaP HEPCGN_14485 0.34 -1.3 1,652,360 + rfaP HEPCGN_14485 0.35 -1.6 1,652,361 - rfaP HEPCGN_14485 0.35 -0.4 1,652,361 - rfaP HEPCGN_14485 0.35 -1.1 1,652,457 - rfaP HEPCGN_14485 0.47 +3.8 1,652,503 + rfaP HEPCGN_14485 0.53 -1.1 1,652,512 + rfaP HEPCGN_14485 0.54 +0.7 1,652,574 - rfaP HEPCGN_14485 0.61 -1.0 1,652,574 - rfaP HEPCGN_14485 0.61 +1.4 1,652,576 - rfaP HEPCGN_14485 0.62 +0.6 1,652,701 + rfaP HEPCGN_14485 0.77 -2.8 1,652,761 + rfaP HEPCGN_14485 0.85 +2.3 1,652,771 + rfaP HEPCGN_14485 0.86 +0.3 1,653,058 + waaO HEPCGN_14490 0.16 -1.5
Or see this region's nucleotide sequence