Strain Fitness in Escherichia coli ECOR38 around HEPCGN_14380

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspoT and rpoZ are separated by 18 nucleotidesrpoZ and gmk are separated by 54 nucleotidesgmk and ligB are separated by 257 nucleotides HEPCGN_14375: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 1,632,261 to 1,634,369 spoT HEPCGN_14380: rpoZ - DNA-directed RNA polymerase subunit omega, at 1,634,388 to 1,634,663 rpoZ HEPCGN_14385: gmk - guanylate kinase, at 1,634,718 to 1,635,341 gmk HEPCGN_14390: ligB - NAD-dependent DNA ligase LigB, at 1,635,599 to 1,637,281 ligB Position (kb) 1634 1635Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1634.521 kb on - strand, within rpoZat 1634.605 kb on - strand, within rpoZat 1634.653 kb on - strandat 1634.664 kb on - strandat 1635.378 kb on - strandat 1635.382 kb on - strandat 1635.382 kb on - strandat 1635.495 kb on + strandat 1635.565 kb on + strandat 1635.565 kb on + strandat 1635.565 kb on + strandat 1635.566 kb on - strandat 1635.566 kb on - strandat 1635.567 kb on + strandat 1635.568 kb on - strandat 1635.580 kb on - strandat 1635.580 kb on - strandat 1635.621 kb on + strandat 1635.624 kb on + strandat 1635.624 kb on + strandat 1635.624 kb on + strandat 1635.624 kb on + strandat 1635.625 kb on - strandat 1635.628 kb on + strandat 1635.628 kb on + strandat 1635.628 kb on + strandat 1635.629 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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1,634,521 - rpoZ HEPCGN_14380 0.48 +0.9
1,634,605 - rpoZ HEPCGN_14380 0.79 +0.3
1,634,653 - +1.1
1,634,664 - +1.7
1,635,378 - -0.2
1,635,382 - -2.3
1,635,382 - -0.1
1,635,495 + +2.3
1,635,565 + -0.8
1,635,565 + -1.8
1,635,565 + -0.2
1,635,566 - +0.1
1,635,566 - +2.9
1,635,567 + -0.1
1,635,568 - +0.8
1,635,580 - -0.3
1,635,580 - +1.1
1,635,621 + +1.8
1,635,624 + +0.6
1,635,624 + -2.1
1,635,624 + +0.6
1,635,624 + -1.1
1,635,625 - -0.4
1,635,628 + +1.0
1,635,628 + +0.8
1,635,628 + -0.8
1,635,629 - +0.8

Or see this region's nucleotide sequence