Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_12405 and zapB are separated by 38 nucleotides zapB and glpF are separated by 430 nucleotides glpF and glpK are separated by 22 nucleotides
HEPCGN_12405: HEPCGN_12405 - hypothetical protein, at 1,231,781 to 1,232,407
_12405
HEPCGN_12410: zapB - septal ring assembly protein ZapB, at 1,232,446 to 1,232,685
zapB
HEPCGN_12415: glpF - glycerol uptake facilitator protein GlpF, at 1,233,116 to 1,233,961
glpF
HEPCGN_12420: glpK - glycerol kinase GlpK, at 1,233,984 to 1,235,492
glpK
Position (kb)
1233
1234 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1232.177 kb on + strand, within HEPCGN_12405 at 1232.178 kb on - strand, within HEPCGN_12405 at 1232.178 kb on - strand, within HEPCGN_12405 at 1232.178 kb on - strand, within HEPCGN_12405 at 1232.231 kb on - strand, within HEPCGN_12405 at 1232.288 kb on + strand, within HEPCGN_12405 at 1232.328 kb on + strand, within HEPCGN_12405 at 1232.350 kb on + strand at 1232.378 kb on - strand at 1232.677 kb on + strand at 1232.678 kb on - strand at 1232.678 kb on - strand at 1232.723 kb on - strand at 1232.755 kb on + strand at 1232.758 kb on + strand at 1232.759 kb on - strand at 1232.763 kb on - strand at 1232.775 kb on + strand at 1232.791 kb on + strand at 1232.921 kb on - strand at 1232.938 kb on + strand at 1232.938 kb on + strand at 1232.939 kb on - strand at 1232.953 kb on + strand at 1232.998 kb on + strand at 1233.085 kb on - strand at 1233.096 kb on + strand at 1233.096 kb on + strand at 1233.096 kb on + strand at 1233.096 kb on + strand at 1233.097 kb on - strand at 1233.210 kb on + strand, within glpF at 1233.211 kb on - strand, within glpF at 1233.284 kb on + strand, within glpF at 1233.284 kb on + strand, within glpF at 1233.285 kb on - strand, within glpF at 1233.329 kb on - strand, within glpF at 1233.382 kb on + strand, within glpF at 1233.437 kb on + strand, within glpF at 1233.523 kb on + strand, within glpF at 1233.524 kb on - strand, within glpF at 1233.527 kb on + strand, within glpF at 1233.527 kb on + strand, within glpF at 1233.533 kb on - strand, within glpF at 1233.542 kb on - strand, within glpF at 1233.694 kb on + strand, within glpF at 1233.695 kb on - strand, within glpF at 1233.810 kb on - strand, within glpF at 1233.810 kb on - strand, within glpF at 1233.810 kb on - strand, within glpF at 1233.852 kb on + strand, within glpF at 1233.959 kb on + strand at 1233.960 kb on - strand at 1233.962 kb on + strand at 1233.963 kb on - strand at 1233.963 kb on - strand at 1233.963 kb on - strand at 1233.980 kb on - strand at 1234.121 kb on - strand at 1234.168 kb on + strand, within glpK at 1234.223 kb on + strand, within glpK at 1234.244 kb on + strand, within glpK at 1234.274 kb on + strand, within glpK at 1234.278 kb on + strand, within glpK at 1234.278 kb on + strand, within glpK at 1234.279 kb on - strand, within glpK at 1234.279 kb on - strand, within glpK at 1234.279 kb on - strand, within glpK at 1234.279 kb on - strand, within glpK at 1234.279 kb on - strand, within glpK at 1234.279 kb on - strand, within glpK at 1234.279 kb on - strand, within glpK at 1234.354 kb on - strand, within glpK at 1234.354 kb on - strand, within glpK at 1234.354 kb on - strand, within glpK at 1234.484 kb on + strand, within glpK at 1234.484 kb on + strand, within glpK at 1234.485 kb on - strand, within glpK at 1234.493 kb on + strand, within glpK at 1234.571 kb on + strand, within glpK at 1234.655 kb on - strand, within glpK at 1234.776 kb on - strand, within glpK at 1234.779 kb on + strand, within glpK at 1234.796 kb on + strand, within glpK at 1234.796 kb on + strand, within glpK at 1234.797 kb on - strand, within glpK at 1234.797 kb on - strand, within glpK at 1234.884 kb on + strand, within glpK at 1234.907 kb on + strand, within glpK at 1234.907 kb on + strand, within glpK at 1234.956 kb on - strand, within glpK
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,232,177 + HEPCGN_12405 0.63 +1.1 1,232,178 - HEPCGN_12405 0.63 +1.4 1,232,178 - HEPCGN_12405 0.63 +0.8 1,232,178 - HEPCGN_12405 0.63 -0.3 1,232,231 - HEPCGN_12405 0.72 +2.2 1,232,288 + HEPCGN_12405 0.81 +0.3 1,232,328 + HEPCGN_12405 0.87 +1.0 1,232,350 + +0.6 1,232,378 - -1.0 1,232,677 + +0.1 1,232,678 - +2.1 1,232,678 - -0.6 1,232,723 - +1.7 1,232,755 + +0.5 1,232,758 + +0.3 1,232,759 - -2.3 1,232,763 - -0.6 1,232,775 + +0.2 1,232,791 + +0.1 1,232,921 - +1.6 1,232,938 + -1.5 1,232,938 + +2.2 1,232,939 - -1.1 1,232,953 + -2.8 1,232,998 + -0.4 1,233,085 - -0.0 1,233,096 + -1.2 1,233,096 + -0.6 1,233,096 + -0.3 1,233,096 + -0.6 1,233,097 - +1.1 1,233,210 + glpF HEPCGN_12415 0.11 -0.7 1,233,211 - glpF HEPCGN_12415 0.11 -0.8 1,233,284 + glpF HEPCGN_12415 0.20 -0.4 1,233,284 + glpF HEPCGN_12415 0.20 +1.8 1,233,285 - glpF HEPCGN_12415 0.20 +1.8 1,233,329 - glpF HEPCGN_12415 0.25 +0.7 1,233,382 + glpF HEPCGN_12415 0.31 +2.1 1,233,437 + glpF HEPCGN_12415 0.38 +0.6 1,233,523 + glpF HEPCGN_12415 0.48 -0.7 1,233,524 - glpF HEPCGN_12415 0.48 +1.0 1,233,527 + glpF HEPCGN_12415 0.49 +0.2 1,233,527 + glpF HEPCGN_12415 0.49 +2.4 1,233,533 - glpF HEPCGN_12415 0.49 +0.8 1,233,542 - glpF HEPCGN_12415 0.50 -0.5 1,233,694 + glpF HEPCGN_12415 0.68 +0.7 1,233,695 - glpF HEPCGN_12415 0.68 -0.0 1,233,810 - glpF HEPCGN_12415 0.82 -0.1 1,233,810 - glpF HEPCGN_12415 0.82 +0.4 1,233,810 - glpF HEPCGN_12415 0.82 +3.8 1,233,852 + glpF HEPCGN_12415 0.87 -1.6 1,233,959 + -0.2 1,233,960 - -1.4 1,233,962 + -1.1 1,233,963 - +1.1 1,233,963 - -0.2 1,233,963 - +1.5 1,233,980 - +1.4 1,234,121 - +0.0 1,234,168 + glpK HEPCGN_12420 0.12 +1.7 1,234,223 + glpK HEPCGN_12420 0.16 +0.8 1,234,244 + glpK HEPCGN_12420 0.17 -0.0 1,234,274 + glpK HEPCGN_12420 0.19 -0.1 1,234,278 + glpK HEPCGN_12420 0.19 +0.7 1,234,278 + glpK HEPCGN_12420 0.19 -2.0 1,234,279 - glpK HEPCGN_12420 0.20 -0.2 1,234,279 - glpK HEPCGN_12420 0.20 +0.0 1,234,279 - glpK HEPCGN_12420 0.20 +0.6 1,234,279 - glpK HEPCGN_12420 0.20 -0.2 1,234,279 - glpK HEPCGN_12420 0.20 +0.4 1,234,279 - glpK HEPCGN_12420 0.20 +1.0 1,234,279 - glpK HEPCGN_12420 0.20 -0.6 1,234,354 - glpK HEPCGN_12420 0.25 +0.0 1,234,354 - glpK HEPCGN_12420 0.25 +0.5 1,234,354 - glpK HEPCGN_12420 0.25 +1.1 1,234,484 + glpK HEPCGN_12420 0.33 +1.1 1,234,484 + glpK HEPCGN_12420 0.33 -0.0 1,234,485 - glpK HEPCGN_12420 0.33 +0.4 1,234,493 + glpK HEPCGN_12420 0.34 -0.2 1,234,571 + glpK HEPCGN_12420 0.39 -0.4 1,234,655 - glpK HEPCGN_12420 0.44 +0.6 1,234,776 - glpK HEPCGN_12420 0.52 -0.3 1,234,779 + glpK HEPCGN_12420 0.53 +1.0 1,234,796 + glpK HEPCGN_12420 0.54 +1.0 1,234,796 + glpK HEPCGN_12420 0.54 +0.0 1,234,797 - glpK HEPCGN_12420 0.54 -0.2 1,234,797 - glpK HEPCGN_12420 0.54 +1.1 1,234,884 + glpK HEPCGN_12420 0.60 -0.6 1,234,907 + glpK HEPCGN_12420 0.61 -0.9 1,234,907 + glpK HEPCGN_12420 0.61 +0.8 1,234,956 - glpK HEPCGN_12420 0.64 -0.1
Or see this region's nucleotide sequence