Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tuf and Thr_trna are separated by 114 nucleotides Thr_trna and Gly_trna are separated by 6 nucleotides Gly_trna and Tyr_trna are separated by 116 nucleotides Tyr_trna and Thr_trna are separated by 8 nucleotides Thr_trna and coaA are separated by 385 nucleotides
HEPCGN_12110: tuf - elongation factor Tu, at 1,165,844 to 1,167,028
tuf
HEPCGN_12115: Thr_trna - tRNA-Thr(ggt), at 1,167,143 to 1,167,218
_trna
HEPCGN_12120: Gly_trna - tRNA-Gly(tcc), at 1,167,225 to 1,167,299
_trna
HEPCGN_12125: Tyr_trna - tRNA-Tyr(gta), at 1,167,416 to 1,167,500
_trna
HEPCGN_12130: Thr_trna - tRNA-Thr(tgt), at 1,167,509 to 1,167,584
_trna
HEPCGN_12135: coaA - type I pantothenate kinase, at 1,167,970 to 1,168,896
coaA
Position (kb)
1167
1168 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1166.799 kb on + strand, within tuf at 1166.957 kb on - strand at 1166.957 kb on - strand at 1166.980 kb on + strand at 1167.004 kb on + strand at 1167.064 kb on - strand at 1167.108 kb on + strand at 1167.210 kb on + strand, within Thr_trna at 1167.210 kb on + strand, within Thr_trna at 1167.382 kb on - strand at 1167.382 kb on - strand at 1167.757 kb on + strand at 1167.780 kb on - strand at 1167.804 kb on + strand at 1167.805 kb on - strand at 1167.833 kb on - strand at 1167.925 kb on - strand at 1167.925 kb on - strand at 1167.925 kb on - strand at 1167.945 kb on + strand at 1167.945 kb on + strand at 1167.945 kb on + strand at 1167.945 kb on + strand at 1167.946 kb on - strand at 1167.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,166,799 + tuf HEPCGN_12110 0.81 -1.8 1,166,957 - -1.6 1,166,957 - +0.2 1,166,980 + -0.1 1,167,004 + +0.6 1,167,064 - -0.6 1,167,108 + +3.8 1,167,210 + Thr_trna HEPCGN_12115 0.88 -1.1 1,167,210 + Thr_trna HEPCGN_12115 0.88 +3.8 1,167,382 - -1.0 1,167,382 - -0.8 1,167,757 + -1.6 1,167,780 - +0.9 1,167,804 + -0.5 1,167,805 - +3.4 1,167,833 - -0.7 1,167,925 - -0.3 1,167,925 - -0.6 1,167,925 - -0.1 1,167,945 + -0.2 1,167,945 + +1.3 1,167,945 + -0.2 1,167,945 + +3.3 1,167,946 - +0.2 1,167,953 + -0.4
Or see this region's nucleotide sequence