Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppa and chpB are separated by 78 nucleotides chpB and chpS are separated by 29 nucleotides chpS and ytfP are separated by 211 nucleotides ytfP and tamB are separated by 2 nucleotides
HEPCGN_10175: ppa - inorganic diphosphatase, at 780,964 to 781,494
ppa
HEPCGN_10180: chpB - type II toxin-antitoxin system ChpB family toxin, at 781,573 to 781,887
chpB
HEPCGN_10185: chpS - type II toxin-antitoxin system antitoxin ChpS, at 781,917 to 782,168
chpS
HEPCGN_10190: ytfP - Gamma-glutamylcyclotransferase family protein YtfP, at 782,380 to 782,721
ytfP
HEPCGN_10195: tamB - autotransporter assembly complex protein TamB, at 782,724 to 786,503
tamB
Position (kb) 781
782
783 Strain fitness (log2 ratio) -2
-1
0
1
2 at 780.922 kb on + strand at 780.922 kb on + strand at 781.493 kb on - strand at 781.508 kb on - strand at 781.560 kb on - strand at 781.563 kb on + strand at 781.598 kb on - strand at 781.598 kb on - strand at 781.598 kb on - strand at 781.661 kb on - strand, within chpB at 781.723 kb on + strand, within chpB at 781.734 kb on + strand, within chpB at 781.734 kb on + strand, within chpB at 781.950 kb on - strand, within chpS at 782.021 kb on - strand, within chpS at 782.155 kb on - strand at 782.162 kb on + strand at 782.162 kb on + strand at 782.163 kb on - strand at 782.163 kb on - strand at 782.196 kb on + strand at 782.274 kb on - strand at 782.315 kb on + strand at 782.381 kb on + strand at 782.381 kb on + strand at 782.381 kb on + strand at 782.381 kb on + strand at 782.382 kb on - strand at 782.385 kb on + strand at 782.386 kb on - strand at 782.450 kb on + strand, within ytfP at 782.450 kb on + strand, within ytfP at 782.451 kb on - strand, within ytfP at 782.451 kb on - strand, within ytfP at 782.569 kb on + strand, within ytfP at 782.615 kb on + strand, within ytfP at 782.615 kb on + strand, within ytfP at 782.615 kb on + strand, within ytfP at 782.616 kb on - strand, within ytfP at 782.616 kb on - strand, within ytfP at 782.625 kb on - strand, within ytfP at 782.696 kb on - strand at 782.763 kb on + strand at 782.785 kb on + strand at 782.787 kb on + strand at 782.788 kb on - strand at 782.788 kb on - strand at 782.792 kb on + strand at 782.793 kb on - strand at 782.793 kb on - strand at 782.827 kb on - strand at 782.827 kb on - strand at 782.828 kb on + strand at 782.829 kb on - strand at 782.840 kb on + strand at 782.841 kb on - strand at 782.874 kb on + strand at 782.874 kb on + strand at 782.874 kb on + strand at 782.874 kb on + strand at 782.875 kb on - strand at 782.878 kb on + strand at 782.931 kb on + strand at 782.941 kb on - strand at 783.061 kb on - strand at 783.160 kb on - strand, within tamB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 780,922 + +0.4 780,922 + +2.1 781,493 - +0.4 781,508 - +0.7 781,560 - +2.2 781,563 + -0.5 781,598 - -2.0 781,598 - +0.1 781,598 - +0.3 781,661 - chpB HEPCGN_10180 0.28 +0.5 781,723 + chpB HEPCGN_10180 0.48 +1.7 781,734 + chpB HEPCGN_10180 0.51 +2.4 781,734 + chpB HEPCGN_10180 0.51 +0.6 781,950 - chpS HEPCGN_10185 0.13 -0.2 782,021 - chpS HEPCGN_10185 0.41 -0.7 782,155 - +0.3 782,162 + -0.7 782,162 + -0.1 782,163 - +0.0 782,163 - +0.0 782,196 + -0.2 782,274 - +0.1 782,315 + -0.5 782,381 + +1.2 782,381 + +1.0 782,381 + -0.3 782,381 + -0.4 782,382 - +2.2 782,385 + +0.4 782,386 - -1.0 782,450 + ytfP HEPCGN_10190 0.20 +0.6 782,450 + ytfP HEPCGN_10190 0.20 -0.3 782,451 - ytfP HEPCGN_10190 0.21 +1.0 782,451 - ytfP HEPCGN_10190 0.21 +0.6 782,569 + ytfP HEPCGN_10190 0.55 -1.7 782,615 + ytfP HEPCGN_10190 0.69 +0.1 782,615 + ytfP HEPCGN_10190 0.69 -0.7 782,615 + ytfP HEPCGN_10190 0.69 +0.1 782,616 - ytfP HEPCGN_10190 0.69 +0.9 782,616 - ytfP HEPCGN_10190 0.69 +1.0 782,625 - ytfP HEPCGN_10190 0.72 +0.0 782,696 - -1.3 782,763 + -0.1 782,785 + +2.6 782,787 + -1.4 782,788 - +0.1 782,788 - -1.8 782,792 + -0.4 782,793 - +0.1 782,793 - +0.8 782,827 - -0.9 782,827 - +0.5 782,828 + -2.0 782,829 - +0.9 782,840 + +0.7 782,841 - -0.2 782,874 + +0.4 782,874 + -1.7 782,874 + +0.3 782,874 + -1.1 782,875 - -0.7 782,878 + +0.0 782,931 + -1.0 782,941 - -1.1 783,061 - +1.3 783,160 - tamB HEPCGN_10195 0.12 +0.8
Or see this region's nucleotide sequence