Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrB and pyrI are separated by 12 nucleotides pyrI and ridA are separated by 72 nucleotides ridA and mgtA are separated by 205 nucleotides
HEPCGN_10060: pyrB - aspartate carbamoyltransferase, at 757,777 to 758,712
pyrB
HEPCGN_10065: pyrI - aspartate carbamoyltransferase regulatory subunit, at 758,725 to 759,186
pyrI
HEPCGN_10070: ridA - 2-iminobutanoate/2-iminopropanoate deaminase, at 759,259 to 759,645
ridA
HEPCGN_10075: mgtA - magnesium-translocating P-type ATPase, at 759,851 to 762,547
mgtA
Position (kb) 758
759
760 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 757.791 kb on - strand at 757.804 kb on - strand at 757.840 kb on - strand at 757.840 kb on - strand at 757.909 kb on - strand, within pyrB at 757.909 kb on - strand, within pyrB at 758.013 kb on + strand, within pyrB at 758.013 kb on + strand, within pyrB at 758.204 kb on + strand, within pyrB at 758.332 kb on + strand, within pyrB at 758.333 kb on - strand, within pyrB at 758.369 kb on - strand, within pyrB at 758.380 kb on - strand, within pyrB at 758.421 kb on + strand, within pyrB at 758.421 kb on + strand, within pyrB at 758.422 kb on - strand, within pyrB at 758.422 kb on - strand, within pyrB at 758.445 kb on - strand, within pyrB at 758.455 kb on + strand, within pyrB at 758.720 kb on + strand at 758.720 kb on + strand at 758.720 kb on + strand at 758.720 kb on + strand at 758.721 kb on - strand at 758.757 kb on + strand at 758.774 kb on + strand, within pyrI at 758.806 kb on - strand, within pyrI at 758.953 kb on + strand, within pyrI at 758.953 kb on + strand, within pyrI at 758.989 kb on + strand, within pyrI at 758.990 kb on - strand, within pyrI at 759.066 kb on + strand, within pyrI at 759.067 kb on - strand, within pyrI at 759.142 kb on + strand at 759.142 kb on + strand at 759.165 kb on + strand at 759.200 kb on + strand at 759.200 kb on + strand at 759.201 kb on - strand at 759.243 kb on + strand at 759.244 kb on - strand at 759.297 kb on + strand at 759.307 kb on + strand, within ridA at 759.311 kb on + strand, within ridA at 759.312 kb on - strand, within ridA at 759.312 kb on - strand, within ridA at 759.312 kb on - strand, within ridA at 759.429 kb on - strand, within ridA at 759.529 kb on + strand, within ridA at 759.529 kb on + strand, within ridA at 759.665 kb on + strand at 759.721 kb on + strand at 759.721 kb on + strand at 759.730 kb on + strand at 759.845 kb on + strand at 759.846 kb on - strand at 759.846 kb on - strand at 759.874 kb on + strand at 759.877 kb on - strand at 759.907 kb on + strand at 759.907 kb on + strand at 759.907 kb on + strand at 759.907 kb on + strand at 759.908 kb on - strand at 759.910 kb on - strand at 759.910 kb on - strand at 759.942 kb on + strand at 759.942 kb on + strand at 759.942 kb on + strand at 759.942 kb on + strand at 759.942 kb on + strand at 759.943 kb on - strand at 759.973 kb on - strand at 760.030 kb on + strand at 760.090 kb on + strand at 760.178 kb on + strand, within mgtA at 760.179 kb on - strand, within mgtA at 760.179 kb on - strand, within mgtA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 757,791 - +1.1 757,804 - +3.1 757,840 - +0.2 757,840 - -1.5 757,909 - pyrB HEPCGN_10060 0.14 -0.3 757,909 - pyrB HEPCGN_10060 0.14 -0.1 758,013 + pyrB HEPCGN_10060 0.25 -0.9 758,013 + pyrB HEPCGN_10060 0.25 -1.7 758,204 + pyrB HEPCGN_10060 0.46 +0.6 758,332 + pyrB HEPCGN_10060 0.59 +0.0 758,333 - pyrB HEPCGN_10060 0.59 +1.0 758,369 - pyrB HEPCGN_10060 0.63 -0.7 758,380 - pyrB HEPCGN_10060 0.64 +0.7 758,421 + pyrB HEPCGN_10060 0.69 -0.4 758,421 + pyrB HEPCGN_10060 0.69 +0.4 758,422 - pyrB HEPCGN_10060 0.69 -0.3 758,422 - pyrB HEPCGN_10060 0.69 -1.5 758,445 - pyrB HEPCGN_10060 0.71 +0.7 758,455 + pyrB HEPCGN_10060 0.72 -2.1 758,720 + -3.8 758,720 + +1.5 758,720 + -0.3 758,720 + +0.4 758,721 - -0.6 758,757 + +0.2 758,774 + pyrI HEPCGN_10065 0.11 -3.1 758,806 - pyrI HEPCGN_10065 0.18 -0.2 758,953 + pyrI HEPCGN_10065 0.49 +1.2 758,953 + pyrI HEPCGN_10065 0.49 +2.3 758,989 + pyrI HEPCGN_10065 0.57 +1.0 758,990 - pyrI HEPCGN_10065 0.57 -0.1 759,066 + pyrI HEPCGN_10065 0.74 +0.6 759,067 - pyrI HEPCGN_10065 0.74 +0.2 759,142 + -0.6 759,142 + +0.9 759,165 + +0.1 759,200 + -3.1 759,200 + +0.2 759,201 - +0.4 759,243 + -0.5 759,244 - -0.2 759,297 + -0.4 759,307 + ridA HEPCGN_10070 0.12 -0.5 759,311 + ridA HEPCGN_10070 0.13 +0.7 759,312 - ridA HEPCGN_10070 0.14 -0.2 759,312 - ridA HEPCGN_10070 0.14 -2.0 759,312 - ridA HEPCGN_10070 0.14 -0.1 759,429 - ridA HEPCGN_10070 0.44 -1.2 759,529 + ridA HEPCGN_10070 0.70 -0.5 759,529 + ridA HEPCGN_10070 0.70 -1.5 759,665 + -0.7 759,721 + +0.4 759,721 + -1.9 759,730 + -1.7 759,845 + -1.0 759,846 - +0.6 759,846 - +1.6 759,874 + +1.1 759,877 - -0.1 759,907 + -0.3 759,907 + -0.6 759,907 + +0.0 759,907 + -2.5 759,908 - -1.2 759,910 - -0.6 759,910 - -1.1 759,942 + -0.3 759,942 + +0.6 759,942 + +3.7 759,942 + +0.8 759,942 + -1.0 759,943 - +0.6 759,973 - +0.1 760,030 + +0.2 760,090 + -0.2 760,178 + mgtA HEPCGN_10075 0.12 -0.2 760,179 - mgtA HEPCGN_10075 0.12 +1.6 760,179 - mgtA HEPCGN_10075 0.12 +1.5
Or see this region's nucleotide sequence