Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt robA and HEPCGN_09160 overlap by 4 nucleotides HEPCGN_09160 and yjjX are separated by 51 nucleotides yjjX and trpR overlap by 7 nucleotides trpR and sltY are separated by 119 nucleotides
HEPCGN_09155: robA - MDR efflux pump AcrAB transcriptional activator RobA, at 578,965 to 579,834
robA
HEPCGN_09160: HEPCGN_09160 - putative phosphoglycerate mutase GpmB, at 579,831 to 580,478
_09160
HEPCGN_09165: yjjX - inosine/xanthosine triphosphatase, at 580,530 to 581,045
yjjX
HEPCGN_09170: trpR - trp operon repressor, at 581,039 to 581,335
trpR
HEPCGN_09175: sltY - murein transglycosylase, at 581,455 to 583,392
sltY
Position (kb) 580
581
582 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 579.556 kb on + strand, within robA at 579.556 kb on + strand, within robA at 579.557 kb on - strand, within robA at 579.557 kb on - strand, within robA at 579.596 kb on + strand, within robA at 579.596 kb on + strand, within robA at 579.596 kb on + strand, within robA at 579.596 kb on + strand, within robA at 579.597 kb on - strand, within robA at 579.597 kb on - strand, within robA at 579.597 kb on - strand, within robA at 579.635 kb on - strand, within robA at 579.645 kb on + strand, within robA at 579.650 kb on - strand, within robA at 579.694 kb on + strand, within robA at 579.800 kb on + strand at 579.849 kb on + strand at 579.849 kb on + strand at 579.850 kb on - strand at 579.963 kb on + strand, within HEPCGN_09160 at 580.203 kb on - strand, within HEPCGN_09160 at 580.218 kb on + strand, within HEPCGN_09160 at 580.230 kb on + strand, within HEPCGN_09160 at 580.231 kb on - strand, within HEPCGN_09160 at 580.311 kb on + strand, within HEPCGN_09160 at 580.328 kb on + strand, within HEPCGN_09160 at 580.329 kb on - strand, within HEPCGN_09160 at 580.335 kb on - strand, within HEPCGN_09160 at 580.462 kb on - strand at 580.462 kb on - strand at 580.465 kb on + strand at 580.465 kb on + strand at 580.466 kb on - strand at 580.467 kb on + strand at 580.467 kb on + strand at 580.468 kb on - strand at 580.492 kb on - strand at 580.507 kb on + strand at 580.520 kb on - strand at 580.529 kb on + strand at 580.623 kb on - strand, within yjjX at 580.911 kb on - strand, within yjjX at 580.916 kb on + strand, within yjjX at 580.990 kb on - strand, within yjjX at 581.027 kb on + strand at 581.108 kb on - strand, within trpR at 581.258 kb on + strand, within trpR at 581.259 kb on - strand, within trpR at 581.259 kb on - strand, within trpR at 581.278 kb on - strand, within trpR at 581.346 kb on + strand at 581.365 kb on + strand at 581.365 kb on + strand at 581.368 kb on + strand at 581.438 kb on + strand at 581.444 kb on - strand at 581.532 kb on - strand at 581.577 kb on + strand at 581.655 kb on + strand, within sltY at 581.705 kb on + strand, within sltY at 581.769 kb on + strand, within sltY at 581.770 kb on - strand, within sltY at 581.781 kb on + strand, within sltY
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 579,556 + robA HEPCGN_09155 0.68 -1.1 579,556 + robA HEPCGN_09155 0.68 -0.2 579,557 - robA HEPCGN_09155 0.68 -1.2 579,557 - robA HEPCGN_09155 0.68 -1.6 579,596 + robA HEPCGN_09155 0.73 +0.6 579,596 + robA HEPCGN_09155 0.73 -0.1 579,596 + robA HEPCGN_09155 0.73 +0.9 579,596 + robA HEPCGN_09155 0.73 -0.1 579,597 - robA HEPCGN_09155 0.73 -2.4 579,597 - robA HEPCGN_09155 0.73 -2.8 579,597 - robA HEPCGN_09155 0.73 +2.7 579,635 - robA HEPCGN_09155 0.77 +1.7 579,645 + robA HEPCGN_09155 0.78 -0.6 579,650 - robA HEPCGN_09155 0.79 -0.9 579,694 + robA HEPCGN_09155 0.84 +0.7 579,800 + +0.7 579,849 + -0.8 579,849 + +0.2 579,850 - +0.2 579,963 + HEPCGN_09160 0.20 -1.3 580,203 - HEPCGN_09160 0.57 +0.4 580,218 + HEPCGN_09160 0.60 -0.4 580,230 + HEPCGN_09160 0.62 -0.8 580,231 - HEPCGN_09160 0.62 -0.8 580,311 + HEPCGN_09160 0.74 +0.2 580,328 + HEPCGN_09160 0.77 +0.2 580,329 - HEPCGN_09160 0.77 +0.0 580,335 - HEPCGN_09160 0.78 +0.5 580,462 - +0.4 580,462 - -0.3 580,465 + -0.4 580,465 + +1.4 580,466 - -0.1 580,467 + +0.3 580,467 + -1.9 580,468 - -0.6 580,492 - -0.8 580,507 + -0.6 580,520 - -0.1 580,529 + +0.8 580,623 - yjjX HEPCGN_09165 0.18 +1.5 580,911 - yjjX HEPCGN_09165 0.74 +0.1 580,916 + yjjX HEPCGN_09165 0.75 +1.3 580,990 - yjjX HEPCGN_09165 0.89 -1.7 581,027 + -0.3 581,108 - trpR HEPCGN_09170 0.23 +0.2 581,258 + trpR HEPCGN_09170 0.74 +2.7 581,259 - trpR HEPCGN_09170 0.74 -0.8 581,259 - trpR HEPCGN_09170 0.74 +1.4 581,278 - trpR HEPCGN_09170 0.80 -0.6 581,346 + +0.2 581,365 + +1.9 581,365 + +0.2 581,368 + +0.3 581,438 + -0.5 581,444 - -1.8 581,532 - +0.9 581,577 + +0.4 581,655 + sltY HEPCGN_09175 0.10 -1.2 581,705 + sltY HEPCGN_09175 0.13 -0.5 581,769 + sltY HEPCGN_09175 0.16 +2.3 581,770 - sltY HEPCGN_09175 0.16 +1.0 581,781 + sltY HEPCGN_09175 0.17 -0.1
Or see this region's nucleotide sequence