Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaH and HEPCGN_08840 are separated by 185 nucleotides HEPCGN_08840 and HEPCGN_08845 are separated by 2 nucleotides HEPCGN_08845 and HEPCGN_08850 are separated by 85 nucleotides HEPCGN_08850 and kefC are separated by 80 nucleotides
HEPCGN_08835: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 511,194 to 512,042
apaH
HEPCGN_08840: HEPCGN_08840 - Toxin CcdB, at 512,228 to 512,353
_08840
HEPCGN_08845: HEPCGN_08845 - antitoxin, at 512,356 to 512,589
_08845
HEPCGN_08850: HEPCGN_08850 - Dihydrofolate reductase, at 512,675 to 513,265
_08850
HEPCGN_08855: kefC - glutathione-regulated potassium-efflux system protein KefC, at 513,346 to 515,208
kefC
Position (kb) 512
513 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 511.230 kb on + strand at 511.231 kb on - strand at 511.256 kb on + strand at 511.261 kb on + strand at 511.269 kb on - strand at 511.283 kb on + strand, within apaH at 511.369 kb on + strand, within apaH at 511.370 kb on - strand, within apaH at 511.370 kb on - strand, within apaH at 511.434 kb on - strand, within apaH at 511.491 kb on - strand, within apaH at 511.609 kb on + strand, within apaH at 511.610 kb on - strand, within apaH at 511.610 kb on - strand, within apaH at 511.635 kb on + strand, within apaH at 511.659 kb on + strand, within apaH at 511.660 kb on - strand, within apaH at 511.772 kb on + strand, within apaH at 511.776 kb on + strand, within apaH at 511.776 kb on + strand, within apaH at 511.946 kb on + strand, within apaH at 511.947 kb on - strand, within apaH at 511.973 kb on + strand at 511.974 kb on - strand at 511.981 kb on - strand at 512.164 kb on - strand at 512.164 kb on - strand at 512.178 kb on - strand at 512.178 kb on - strand at 512.183 kb on + strand at 512.183 kb on + strand at 512.237 kb on - strand at 512.291 kb on - strand, within HEPCGN_08840 at 512.291 kb on - strand, within HEPCGN_08840 at 512.340 kb on - strand, within HEPCGN_08840 at 512.506 kb on - strand, within HEPCGN_08845 at 512.509 kb on + strand, within HEPCGN_08845 at 512.575 kb on - strand at 512.613 kb on - strand at 512.620 kb on + strand at 512.620 kb on + strand at 512.620 kb on + strand at 512.621 kb on - strand at 512.623 kb on + strand at 512.623 kb on + strand at 512.624 kb on - strand at 512.624 kb on - strand at 512.625 kb on + strand at 512.633 kb on + strand at 512.643 kb on + strand at 512.643 kb on + strand at 512.644 kb on - strand at 512.644 kb on - strand at 512.647 kb on - strand at 512.647 kb on - strand at 512.664 kb on - strand at 512.668 kb on + strand at 512.668 kb on + strand at 512.669 kb on - strand at 513.189 kb on + strand, within HEPCGN_08850 at 513.190 kb on - strand, within HEPCGN_08850 at 513.190 kb on - strand, within HEPCGN_08850 at 513.190 kb on - strand, within HEPCGN_08850 at 513.190 kb on - strand, within HEPCGN_08850 at 513.190 kb on - strand, within HEPCGN_08850 at 513.345 kb on - strand at 513.347 kb on + strand at 513.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 511,230 + +0.6 511,231 - +0.6 511,256 + -1.5 511,261 + -2.8 511,269 - -0.1 511,283 + apaH HEPCGN_08835 0.10 -0.5 511,369 + apaH HEPCGN_08835 0.21 -2.1 511,370 - apaH HEPCGN_08835 0.21 -1.0 511,370 - apaH HEPCGN_08835 0.21 -0.1 511,434 - apaH HEPCGN_08835 0.28 -2.7 511,491 - apaH HEPCGN_08835 0.35 +0.0 511,609 + apaH HEPCGN_08835 0.49 +0.8 511,610 - apaH HEPCGN_08835 0.49 -3.8 511,610 - apaH HEPCGN_08835 0.49 -3.4 511,635 + apaH HEPCGN_08835 0.52 -2.6 511,659 + apaH HEPCGN_08835 0.55 +0.2 511,660 - apaH HEPCGN_08835 0.55 -2.0 511,772 + apaH HEPCGN_08835 0.68 -0.5 511,776 + apaH HEPCGN_08835 0.69 -2.1 511,776 + apaH HEPCGN_08835 0.69 -1.3 511,946 + apaH HEPCGN_08835 0.89 +2.6 511,947 - apaH HEPCGN_08835 0.89 -2.9 511,973 + -0.9 511,974 - -2.1 511,981 - -1.0 512,164 - -0.3 512,164 - -0.5 512,178 - -1.0 512,178 - +0.1 512,183 + +0.2 512,183 + -0.1 512,237 - +0.3 512,291 - HEPCGN_08840 0.50 -0.3 512,291 - HEPCGN_08840 0.50 +2.6 512,340 - HEPCGN_08840 0.89 +1.1 512,506 - HEPCGN_08845 0.64 -1.4 512,509 + HEPCGN_08845 0.65 +0.8 512,575 - +0.0 512,613 - +0.5 512,620 + +0.6 512,620 + +1.1 512,620 + -0.8 512,621 - +0.5 512,623 + +0.7 512,623 + -0.8 512,624 - -1.1 512,624 - -1.8 512,625 + -0.4 512,633 + -0.2 512,643 + +0.7 512,643 + +0.4 512,644 - +1.5 512,644 - -1.0 512,647 - -0.0 512,647 - +0.0 512,664 - +0.1 512,668 + -2.2 512,668 + -1.2 512,669 - +0.1 513,189 + HEPCGN_08850 0.87 +0.6 513,190 - HEPCGN_08850 0.87 +0.3 513,190 - HEPCGN_08850 0.87 +0.8 513,190 - HEPCGN_08850 0.87 -0.1 513,190 - HEPCGN_08850 0.87 +0.1 513,190 - HEPCGN_08850 0.87 -0.2 513,345 - -0.2 513,347 + +0.3 513,347 + +0.7
Or see this region's nucleotide sequence