Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speD and yacL are separated by 37 nucleotides yacL and acnB are separated by 174 nucleotides acnB and HEPCGN_08465 are separated by 354 nucleotides
HEPCGN_08450: speD - adenosylmethionine decarboxylase, at 427,146 to 427,940
speD
HEPCGN_08455: yacL - protein YacL, at 427,978 to 428,340
yacL
HEPCGN_08460: acnB - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 428,515 to 431,112
acnB
HEPCGN_08465: HEPCGN_08465 - DUF3300 domain-containing protein, at 431,467 to 433,224
_08465
Position (kb) 428
429
430
431
432 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 427.528 kb on + strand, within speD at 427.528 kb on + strand, within speD at 427.529 kb on - strand, within speD at 427.529 kb on - strand, within speD at 427.529 kb on - strand, within speD at 427.532 kb on + strand, within speD at 427.596 kb on + strand, within speD at 427.628 kb on - strand, within speD at 427.673 kb on + strand, within speD at 427.700 kb on + strand, within speD at 427.701 kb on - strand, within speD at 427.719 kb on - strand, within speD at 427.818 kb on - strand, within speD at 427.824 kb on + strand, within speD at 427.824 kb on + strand, within speD at 427.825 kb on - strand, within speD at 427.825 kb on - strand, within speD at 427.912 kb on - strand at 427.915 kb on - strand at 427.926 kb on - strand at 427.979 kb on + strand at 428.064 kb on - strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.067 kb on - strand, within yacL at 428.153 kb on + strand, within yacL at 428.153 kb on + strand, within yacL at 428.153 kb on + strand, within yacL at 428.153 kb on + strand, within yacL at 428.154 kb on - strand, within yacL at 428.188 kb on - strand, within yacL at 428.316 kb on + strand at 428.341 kb on - strand at 428.341 kb on - strand at 428.394 kb on + strand at 428.395 kb on - strand at 428.395 kb on - strand at 428.441 kb on + strand at 428.510 kb on - strand at 428.517 kb on - strand at 428.596 kb on - strand at 428.610 kb on + strand at 428.610 kb on + strand at 428.611 kb on - strand at 428.614 kb on + strand at 428.776 kb on + strand, within acnB at 428.852 kb on + strand, within acnB at 428.852 kb on + strand, within acnB at 428.856 kb on - strand, within acnB at 428.976 kb on + strand, within acnB at 428.977 kb on - strand, within acnB at 429.023 kb on - strand, within acnB at 429.246 kb on + strand, within acnB at 429.247 kb on - strand, within acnB at 429.247 kb on - strand, within acnB at 429.258 kb on - strand, within acnB at 429.291 kb on + strand, within acnB at 429.292 kb on - strand, within acnB at 429.446 kb on + strand, within acnB at 429.531 kb on + strand, within acnB at 429.597 kb on + strand, within acnB at 429.598 kb on - strand, within acnB at 429.658 kb on - strand, within acnB at 430.017 kb on + strand, within acnB at 430.332 kb on + strand, within acnB at 430.332 kb on + strand, within acnB at 430.345 kb on - strand, within acnB at 430.409 kb on + strand, within acnB at 430.676 kb on - strand, within acnB at 430.696 kb on - strand, within acnB at 430.718 kb on - strand, within acnB at 430.724 kb on + strand, within acnB at 430.724 kb on + strand, within acnB at 430.823 kb on + strand, within acnB at 430.823 kb on + strand, within acnB at 430.824 kb on - strand, within acnB at 430.928 kb on + strand at 430.928 kb on + strand at 430.928 kb on + strand at 430.929 kb on - strand at 431.095 kb on - strand at 431.095 kb on - strand at 431.192 kb on - strand at 431.296 kb on + strand at 431.388 kb on - strand at 431.388 kb on - strand at 431.398 kb on + strand at 431.399 kb on - strand at 431.466 kb on + strand at 431.546 kb on + strand at 431.547 kb on - strand at 431.548 kb on + strand at 431.548 kb on + strand at 431.821 kb on - strand, within HEPCGN_08465 at 431.838 kb on + strand, within HEPCGN_08465 at 432.084 kb on - strand, within HEPCGN_08465 at 432.109 kb on - strand, within HEPCGN_08465
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 427,528 + speD HEPCGN_08450 0.48 -0.4 427,528 + speD HEPCGN_08450 0.48 +4.0 427,529 - speD HEPCGN_08450 0.48 +0.4 427,529 - speD HEPCGN_08450 0.48 -0.4 427,529 - speD HEPCGN_08450 0.48 +0.8 427,532 + speD HEPCGN_08450 0.49 -0.4 427,596 + speD HEPCGN_08450 0.57 -0.5 427,628 - speD HEPCGN_08450 0.61 -2.7 427,673 + speD HEPCGN_08450 0.66 +0.7 427,700 + speD HEPCGN_08450 0.70 -0.2 427,701 - speD HEPCGN_08450 0.70 -0.4 427,719 - speD HEPCGN_08450 0.72 +1.5 427,818 - speD HEPCGN_08450 0.85 -0.2 427,824 + speD HEPCGN_08450 0.85 -0.3 427,824 + speD HEPCGN_08450 0.85 +0.1 427,825 - speD HEPCGN_08450 0.85 -0.5 427,825 - speD HEPCGN_08450 0.85 -0.7 427,912 - -1.3 427,915 - +0.3 427,926 - -0.5 427,979 + -1.0 428,064 - yacL HEPCGN_08455 0.24 -0.8 428,066 + yacL HEPCGN_08455 0.24 +1.6 428,066 + yacL HEPCGN_08455 0.24 +0.2 428,066 + yacL HEPCGN_08455 0.24 -0.8 428,066 + yacL HEPCGN_08455 0.24 -1.9 428,066 + yacL HEPCGN_08455 0.24 -0.9 428,067 - yacL HEPCGN_08455 0.25 +0.3 428,153 + yacL HEPCGN_08455 0.48 +0.0 428,153 + yacL HEPCGN_08455 0.48 +0.7 428,153 + yacL HEPCGN_08455 0.48 -0.6 428,153 + yacL HEPCGN_08455 0.48 -3.0 428,154 - yacL HEPCGN_08455 0.48 -0.1 428,188 - yacL HEPCGN_08455 0.58 +0.1 428,316 + +0.9 428,341 - +1.1 428,341 - -1.0 428,394 + -0.3 428,395 - +1.0 428,395 - +0.1 428,441 + +0.1 428,510 - -0.3 428,517 - +1.2 428,596 - +0.9 428,610 + -0.7 428,610 + +0.6 428,611 - -3.1 428,614 + -1.5 428,776 + acnB HEPCGN_08460 0.10 +1.3 428,852 + acnB HEPCGN_08460 0.13 -0.9 428,852 + acnB HEPCGN_08460 0.13 +1.6 428,856 - acnB HEPCGN_08460 0.13 +1.3 428,976 + acnB HEPCGN_08460 0.18 +2.0 428,977 - acnB HEPCGN_08460 0.18 +1.0 429,023 - acnB HEPCGN_08460 0.20 +0.7 429,246 + acnB HEPCGN_08460 0.28 +0.0 429,247 - acnB HEPCGN_08460 0.28 -0.5 429,247 - acnB HEPCGN_08460 0.28 -1.6 429,258 - acnB HEPCGN_08460 0.29 +0.1 429,291 + acnB HEPCGN_08460 0.30 +0.3 429,292 - acnB HEPCGN_08460 0.30 -0.5 429,446 + acnB HEPCGN_08460 0.36 -1.1 429,531 + acnB HEPCGN_08460 0.39 -1.1 429,597 + acnB HEPCGN_08460 0.42 +0.6 429,598 - acnB HEPCGN_08460 0.42 +2.3 429,658 - acnB HEPCGN_08460 0.44 +0.0 430,017 + acnB HEPCGN_08460 0.58 +1.3 430,332 + acnB HEPCGN_08460 0.70 +3.1 430,332 + acnB HEPCGN_08460 0.70 +0.1 430,345 - acnB HEPCGN_08460 0.70 +0.1 430,409 + acnB HEPCGN_08460 0.73 -1.2 430,676 - acnB HEPCGN_08460 0.83 +1.3 430,696 - acnB HEPCGN_08460 0.84 +1.0 430,718 - acnB HEPCGN_08460 0.85 +0.4 430,724 + acnB HEPCGN_08460 0.85 +1.0 430,724 + acnB HEPCGN_08460 0.85 +0.0 430,823 + acnB HEPCGN_08460 0.89 +3.6 430,823 + acnB HEPCGN_08460 0.89 +0.3 430,824 - acnB HEPCGN_08460 0.89 -1.5 430,928 + +3.6 430,928 + -0.2 430,928 + +1.6 430,929 - -0.4 431,095 - -0.2 431,095 - -0.7 431,192 - +1.2 431,296 + -1.0 431,388 - -0.6 431,388 - -1.0 431,398 + +0.2 431,399 - +0.1 431,466 + +1.3 431,546 + +0.1 431,547 - -0.3 431,548 + +0.5 431,548 + +1.4 431,821 - HEPCGN_08465 0.20 -1.6 431,838 + HEPCGN_08465 0.21 +0.5 432,084 - HEPCGN_08465 0.35 +1.1 432,109 - HEPCGN_08465 0.37 -0.1
Or see this region's nucleotide sequence