Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsx and yajD are separated by 37 nucleotides yajD and parE are separated by 87 nucleotides parE and parD overlap by 8 nucleotides parD and secF are separated by 151 nucleotides
HEPCGN_07110: tsx - nucleoside-specific channel-forming protein Tsx, at 155,119 to 156,003
tsx
HEPCGN_07115: yajD - HNH nuclease YajD, at 156,041 to 156,388
yajD
HEPCGN_07120: parE - type II toxin-antitoxin system RelE/ParE family toxin, at 156,476 to 156,757
parE
HEPCGN_07125: parD - Antitoxin ParD, at 156,750 to 156,992
parD
HEPCGN_07130: secF - Protein translocase subunit SecF, at 157,144 to 158,115
secF
Position (kb) 156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 155.799 kb on + strand, within tsx at 155.814 kb on + strand, within tsx at 155.815 kb on - strand, within tsx at 155.815 kb on - strand, within tsx at 155.817 kb on + strand, within tsx at 155.817 kb on + strand, within tsx at 155.817 kb on + strand, within tsx at 155.817 kb on + strand, within tsx at 155.854 kb on + strand, within tsx at 155.869 kb on + strand, within tsx at 155.869 kb on + strand, within tsx at 155.978 kb on - strand at 155.987 kb on + strand at 155.988 kb on - strand at 156.087 kb on + strand, within yajD at 156.088 kb on - strand, within yajD at 156.190 kb on - strand, within yajD at 156.211 kb on + strand, within yajD at 156.236 kb on - strand, within yajD at 156.330 kb on - strand, within yajD at 156.477 kb on + strand at 156.478 kb on - strand at 156.542 kb on + strand, within parE at 156.542 kb on + strand, within parE at 156.543 kb on - strand, within parE at 156.544 kb on + strand, within parE at 156.544 kb on + strand, within parE at 156.544 kb on + strand, within parE at 156.545 kb on - strand, within parE at 156.545 kb on - strand, within parE at 156.549 kb on + strand, within parE at 156.549 kb on + strand, within parE at 156.552 kb on + strand, within parE at 156.665 kb on - strand, within parE at 156.705 kb on + strand, within parE at 156.706 kb on - strand, within parE at 156.712 kb on - strand, within parE at 156.712 kb on - strand, within parE at 156.751 kb on + strand at 156.753 kb on + strand at 156.754 kb on - strand at 156.950 kb on + strand, within parD at 156.954 kb on + strand, within parD at 156.955 kb on - strand, within parD at 157.011 kb on - strand at 157.011 kb on - strand at 157.021 kb on - strand at 157.029 kb on + strand at 157.046 kb on + strand at 157.047 kb on - strand at 157.060 kb on + strand at 157.072 kb on + strand at 157.073 kb on - strand at 157.134 kb on + strand at 157.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 155,799 + tsx HEPCGN_07110 0.77 -0.0 155,814 + tsx HEPCGN_07110 0.79 -0.9 155,815 - tsx HEPCGN_07110 0.79 +0.6 155,815 - tsx HEPCGN_07110 0.79 -1.0 155,817 + tsx HEPCGN_07110 0.79 +0.6 155,817 + tsx HEPCGN_07110 0.79 +0.3 155,817 + tsx HEPCGN_07110 0.79 +0.2 155,817 + tsx HEPCGN_07110 0.79 -0.7 155,854 + tsx HEPCGN_07110 0.83 +0.2 155,869 + tsx HEPCGN_07110 0.85 -0.3 155,869 + tsx HEPCGN_07110 0.85 -0.6 155,978 - -1.1 155,987 + -0.7 155,988 - +0.3 156,087 + yajD HEPCGN_07115 0.13 -0.4 156,088 - yajD HEPCGN_07115 0.14 -0.9 156,190 - yajD HEPCGN_07115 0.43 -0.4 156,211 + yajD HEPCGN_07115 0.49 -1.5 156,236 - yajD HEPCGN_07115 0.56 +0.5 156,330 - yajD HEPCGN_07115 0.83 +0.7 156,477 + -0.9 156,478 - -0.8 156,542 + parE HEPCGN_07120 0.23 -0.1 156,542 + parE HEPCGN_07120 0.23 -0.3 156,543 - parE HEPCGN_07120 0.24 -0.3 156,544 + parE HEPCGN_07120 0.24 +0.3 156,544 + parE HEPCGN_07120 0.24 -0.8 156,544 + parE HEPCGN_07120 0.24 -1.1 156,545 - parE HEPCGN_07120 0.24 -1.4 156,545 - parE HEPCGN_07120 0.24 +0.2 156,549 + parE HEPCGN_07120 0.26 +1.0 156,549 + parE HEPCGN_07120 0.26 -3.4 156,552 + parE HEPCGN_07120 0.27 +0.8 156,665 - parE HEPCGN_07120 0.67 -0.5 156,705 + parE HEPCGN_07120 0.81 +0.1 156,706 - parE HEPCGN_07120 0.82 -0.9 156,712 - parE HEPCGN_07120 0.84 +0.4 156,712 - parE HEPCGN_07120 0.84 -1.9 156,751 + +0.1 156,753 + +0.5 156,754 - +1.1 156,950 + parD HEPCGN_07125 0.82 +0.9 156,954 + parD HEPCGN_07125 0.84 +0.8 156,955 - parD HEPCGN_07125 0.84 +3.5 157,011 - -0.6 157,011 - -0.7 157,021 - -0.5 157,029 + +0.1 157,046 + -0.4 157,047 - -0.9 157,060 + +1.0 157,072 + -0.9 157,073 - +0.6 157,134 + -1.3 157,145 + -0.2
Or see this region's nucleotide sequence