Strain Fitness in Escherichia coli ECOR38 around HEPCGN_06475

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppiB and lpxH are separated by 2 nucleotideslpxH and purE are separated by 117 nucleotidespurE and purK overlap by 4 nucleotides HEPCGN_06465: ppiB - peptidylprolyl isomerase B, at 10,418 to 10,912 ppiB HEPCGN_06470: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 10,915 to 11,637 lpxH HEPCGN_06475: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 11,755 to 12,264 purE HEPCGN_06480: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 12,261 to 13,328 purK Position (kb) 11 12 13Strain fitness (log2 ratio) -3 -2 -1 0 1at 11.817 kb on + strand, within purEat 11.952 kb on + strand, within purEat 12.077 kb on + strand, within purEat 12.077 kb on + strand, within purEat 12.147 kb on - strand, within purEat 12.147 kb on - strand, within purEat 12.302 kb on + strandat 12.303 kb on - strandat 12.433 kb on + strand, within purKat 12.489 kb on - strand, within purKat 12.556 kb on + strand, within purKat 12.557 kb on - strand, within purKat 12.617 kb on + strand, within purKat 12.686 kb on - strand, within purKat 12.710 kb on + strand, within purKat 12.711 kb on - strand, within purKat 12.729 kb on - strand, within purKat 12.916 kb on - strand, within purKat 12.992 kb on + strand, within purKat 13.134 kb on + strand, within purK

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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11,817 + purE HEPCGN_06475 0.12 +0.9
11,952 + purE HEPCGN_06475 0.39 -2.9
12,077 + purE HEPCGN_06475 0.63 -0.7
12,077 + purE HEPCGN_06475 0.63 -1.9
12,147 - purE HEPCGN_06475 0.77 -3.2
12,147 - purE HEPCGN_06475 0.77 -1.4
12,302 + -0.8
12,303 - +0.6
12,433 + purK HEPCGN_06480 0.16 -0.6
12,489 - purK HEPCGN_06480 0.21 -0.7
12,556 + purK HEPCGN_06480 0.28 -2.2
12,557 - purK HEPCGN_06480 0.28 +0.0
12,617 + purK HEPCGN_06480 0.33 -1.0
12,686 - purK HEPCGN_06480 0.40 -0.9
12,710 + purK HEPCGN_06480 0.42 -2.3
12,711 - purK HEPCGN_06480 0.42 -0.1
12,729 - purK HEPCGN_06480 0.44 -2.2
12,916 - purK HEPCGN_06480 0.61 -0.5
12,992 + purK HEPCGN_06480 0.68 -0.9
13,134 + purK HEPCGN_06480 0.82 -0.8

Or see this region's nucleotide sequence