Strain Fitness in Escherichia coli ECOR38 around HEPCGN_06455

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHEPCGN_06445 and ybcJ are separated by 1 nucleotidesybcJ and ybcI are separated by 107 nucleotidesybcI and cysS are separated by 35 nucleotides HEPCGN_06445: HEPCGN_06445 - Bifunctional protein FolD, at 7,114 to 7,980 _06445 HEPCGN_06450: ybcJ - ribosome-associated protein YbcJ, at 7,982 to 8,194 ybcJ HEPCGN_06455: ybcI - Inner membrane protein YbcI, at 8,302 to 8,823 ybcI HEPCGN_06460: cysS - cysteine--tRNA ligase, at 8,859 to 10,244 cysS Position (kb) 8 9Strain fitness (log2 ratio) -2 -1 0 1 2 3at 7.957 kb on + strandat 7.957 kb on + strandat 8.003 kb on - strandat 8.179 kb on - strandat 8.250 kb on - strandat 8.273 kb on - strandat 8.273 kb on - strandat 8.317 kb on - strandat 8.317 kb on - strandat 8.384 kb on + strand, within ybcIat 8.452 kb on + strand, within ybcIat 8.520 kb on - strand, within ybcIat 8.789 kb on + strandat 8.790 kb on - strandat 8.790 kb on - strandat 8.790 kb on - strandat 8.860 kb on + strandat 8.864 kb on + strandat 8.865 kb on - strandat 8.865 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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7,957 + -0.5
7,957 + +0.5
8,003 - -0.4
8,179 - +2.0
8,250 - -0.2
8,273 - -1.4
8,273 - +0.4
8,317 - -0.2
8,317 - -0.2
8,384 + ybcI HEPCGN_06455 0.16 +0.8
8,452 + ybcI HEPCGN_06455 0.29 -0.5
8,520 - ybcI HEPCGN_06455 0.42 +1.2
8,789 + +3.1
8,790 - -0.7
8,790 - -0.4
8,790 - -1.0
8,860 + -2.0
8,864 + -0.6
8,865 - -1.2
8,865 - +1.3

Or see this region's nucleotide sequence