Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfC and speG are separated by 2 nucleotides speG and ynfB are separated by 34 nucleotides ynfB and ynfA are separated by 134 nucleotides ynfA and rspA are separated by 205 nucleotides
HEPCGN_06165: ynfC - YnfC family lipoprotein, at 1,109,037 to 1,109,783
ynfC
HEPCGN_06170: speG - spermidine N1-acetyltransferase, at 1,109,786 to 1,110,346
speG
HEPCGN_06175: ynfB - DUF1283 domain-containing protein, at 1,110,381 to 1,110,722
ynfB
HEPCGN_06180: ynfA - YnfA family protein, at 1,110,857 to 1,111,183
ynfA
HEPCGN_06185: rspA - starvation-sensing protein RspA, at 1,111,389 to 1,112,603
rspA
Position (kb)
1110
1111 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1109.566 kb on + strand, within ynfC at 1109.708 kb on - strand, within ynfC at 1109.728 kb on + strand at 1109.766 kb on - strand at 1109.768 kb on + strand at 1109.782 kb on - strand at 1109.787 kb on + strand at 1109.788 kb on - strand at 1109.788 kb on - strand at 1109.856 kb on - strand, within speG at 1109.876 kb on - strand, within speG at 1109.890 kb on + strand, within speG at 1109.993 kb on - strand, within speG at 1109.995 kb on - strand, within speG at 1110.287 kb on + strand, within speG at 1110.288 kb on - strand, within speG at 1110.292 kb on - strand at 1110.296 kb on + strand at 1110.320 kb on + strand at 1110.358 kb on - strand at 1110.382 kb on + strand at 1110.428 kb on + strand, within ynfB at 1110.736 kb on + strand at 1110.747 kb on - strand at 1110.758 kb on + strand at 1110.800 kb on + strand at 1110.800 kb on + strand at 1110.800 kb on + strand at 1110.803 kb on - strand at 1110.811 kb on + strand at 1110.814 kb on + strand at 1110.814 kb on + strand at 1110.830 kb on + strand at 1110.832 kb on + strand at 1110.832 kb on + strand at 1110.832 kb on + strand at 1110.832 kb on + strand at 1110.832 kb on + strand at 1110.833 kb on - strand at 1110.833 kb on - strand at 1110.833 kb on - strand at 1110.846 kb on - strand at 1110.862 kb on + strand at 1111.044 kb on - strand, within ynfA at 1111.244 kb on - strand at 1111.277 kb on + strand at 1111.434 kb on - strand at 1111.583 kb on + strand, within rspA at 1111.601 kb on + strand, within rspA at 1111.658 kb on + strand, within rspA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,109,566 + ynfC HEPCGN_06165 0.71 +0.1 1,109,708 - ynfC HEPCGN_06165 0.90 -1.0 1,109,728 + -0.2 1,109,766 - +0.1 1,109,768 + -0.4 1,109,782 - -0.5 1,109,787 + -0.2 1,109,788 - +0.3 1,109,788 - +0.6 1,109,856 - speG HEPCGN_06170 0.12 -1.5 1,109,876 - speG HEPCGN_06170 0.16 +0.5 1,109,890 + speG HEPCGN_06170 0.19 -0.5 1,109,993 - speG HEPCGN_06170 0.37 -0.2 1,109,995 - speG HEPCGN_06170 0.37 -0.2 1,110,287 + speG HEPCGN_06170 0.89 -1.4 1,110,288 - speG HEPCGN_06170 0.89 -1.0 1,110,292 - -2.2 1,110,296 + +1.5 1,110,320 + -2.2 1,110,358 - +0.5 1,110,382 + -0.3 1,110,428 + ynfB HEPCGN_06175 0.14 -1.3 1,110,736 + +0.5 1,110,747 - +0.4 1,110,758 + -0.0 1,110,800 + +2.0 1,110,800 + -0.0 1,110,800 + -0.3 1,110,803 - -0.3 1,110,811 + -1.9 1,110,814 + -0.6 1,110,814 + +0.3 1,110,830 + -2.0 1,110,832 + +1.1 1,110,832 + -1.1 1,110,832 + +0.3 1,110,832 + +0.8 1,110,832 + -1.4 1,110,833 - -0.9 1,110,833 - -1.5 1,110,833 - +0.6 1,110,846 - -0.5 1,110,862 + -1.3 1,111,044 - ynfA HEPCGN_06180 0.57 -1.4 1,111,244 - -0.9 1,111,277 + +0.1 1,111,434 - +0.1 1,111,583 + rspA HEPCGN_06185 0.16 -0.9 1,111,601 + rspA HEPCGN_06185 0.17 -2.0 1,111,658 + rspA HEPCGN_06185 0.22 -0.3
Or see this region's nucleotide sequence