Strain Fitness in Escherichia coli ECOR38 around HEPCGN_06165

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfE and ynfD are separated by 198 nucleotidesynfD and ynfC are separated by 71 nucleotidesynfC and speG are separated by 2 nucleotidesspeG and ynfB are separated by 34 nucleotides HEPCGN_06155: ynfE - Putative dimethyl sulfoxide reductase chain YnfE, at 1,106,035 to 1,108,461 ynfE HEPCGN_06160: ynfD - Uncharacterized protein YnfD, at 1,108,660 to 1,108,965 ynfD HEPCGN_06165: ynfC - YnfC family lipoprotein, at 1,109,037 to 1,109,783 ynfC HEPCGN_06170: speG - spermidine N1-acetyltransferase, at 1,109,786 to 1,110,346 speG HEPCGN_06175: ynfB - DUF1283 domain-containing protein, at 1,110,381 to 1,110,722 ynfB Position (kb) 1109 1110Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1108.050 kb on + strand, within ynfEat 1108.143 kb on + strand, within ynfEat 1108.184 kb on - strand, within ynfEat 1108.184 kb on - strand, within ynfEat 1108.245 kb on + strandat 1108.461 kb on + strandat 1108.462 kb on - strandat 1108.498 kb on + strandat 1108.528 kb on - strandat 1108.575 kb on + strandat 1108.575 kb on + strandat 1108.589 kb on + strandat 1108.593 kb on - strandat 1108.610 kb on + strandat 1108.610 kb on + strandat 1108.610 kb on + strandat 1108.611 kb on - strandat 1108.752 kb on + strand, within ynfDat 1108.752 kb on + strand, within ynfDat 1108.815 kb on + strand, within ynfDat 1108.815 kb on + strand, within ynfDat 1108.816 kb on - strand, within ynfDat 1108.816 kb on - strand, within ynfDat 1108.855 kb on - strand, within ynfDat 1108.867 kb on + strand, within ynfDat 1108.869 kb on + strand, within ynfDat 1108.870 kb on - strand, within ynfDat 1108.870 kb on - strand, within ynfDat 1109.017 kb on - strandat 1109.017 kb on - strandat 1109.027 kb on - strandat 1109.037 kb on - strandat 1109.099 kb on - strandat 1109.137 kb on + strand, within ynfCat 1109.138 kb on - strand, within ynfCat 1109.175 kb on - strand, within ynfCat 1109.324 kb on + strand, within ynfCat 1109.344 kb on + strand, within ynfCat 1109.358 kb on + strand, within ynfCat 1109.362 kb on - strand, within ynfCat 1109.566 kb on + strand, within ynfCat 1109.708 kb on - strand, within ynfCat 1109.728 kb on + strandat 1109.766 kb on - strandat 1109.768 kb on + strandat 1109.782 kb on - strandat 1109.787 kb on + strandat 1109.788 kb on - strandat 1109.788 kb on - strandat 1109.856 kb on - strand, within speGat 1109.876 kb on - strand, within speGat 1109.890 kb on + strand, within speGat 1109.993 kb on - strand, within speGat 1109.995 kb on - strand, within speGat 1110.287 kb on + strand, within speGat 1110.288 kb on - strand, within speGat 1110.292 kb on - strandat 1110.296 kb on + strandat 1110.320 kb on + strandat 1110.358 kb on - strandat 1110.382 kb on + strandat 1110.428 kb on + strand, within ynfBat 1110.736 kb on + strandat 1110.747 kb on - strandat 1110.758 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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1,108,050 + ynfE HEPCGN_06155 0.83 +1.1
1,108,143 + ynfE HEPCGN_06155 0.87 +1.0
1,108,184 - ynfE HEPCGN_06155 0.89 -0.4
1,108,184 - ynfE HEPCGN_06155 0.89 +0.1
1,108,245 + +1.5
1,108,461 + -0.5
1,108,462 - -1.5
1,108,498 + -1.9
1,108,528 - +0.2
1,108,575 + +1.2
1,108,575 + -0.7
1,108,589 + -0.0
1,108,593 - -0.4
1,108,610 + -0.5
1,108,610 + -0.4
1,108,610 + +1.3
1,108,611 - -0.3
1,108,752 + ynfD HEPCGN_06160 0.30 -0.3
1,108,752 + ynfD HEPCGN_06160 0.30 +1.5
1,108,815 + ynfD HEPCGN_06160 0.51 -1.4
1,108,815 + ynfD HEPCGN_06160 0.51 +0.4
1,108,816 - ynfD HEPCGN_06160 0.51 -0.6
1,108,816 - ynfD HEPCGN_06160 0.51 +0.2
1,108,855 - ynfD HEPCGN_06160 0.64 +3.0
1,108,867 + ynfD HEPCGN_06160 0.68 -0.0
1,108,869 + ynfD HEPCGN_06160 0.68 -1.3
1,108,870 - ynfD HEPCGN_06160 0.69 -1.7
1,108,870 - ynfD HEPCGN_06160 0.69 +0.4
1,109,017 - -0.6
1,109,017 - +0.1
1,109,027 - -0.0
1,109,037 - -2.0
1,109,099 - -0.4
1,109,137 + ynfC HEPCGN_06165 0.13 +0.4
1,109,138 - ynfC HEPCGN_06165 0.14 -1.7
1,109,175 - ynfC HEPCGN_06165 0.18 -0.2
1,109,324 + ynfC HEPCGN_06165 0.38 +1.3
1,109,344 + ynfC HEPCGN_06165 0.41 +3.2
1,109,358 + ynfC HEPCGN_06165 0.43 +0.7
1,109,362 - ynfC HEPCGN_06165 0.44 +1.0
1,109,566 + ynfC HEPCGN_06165 0.71 +0.1
1,109,708 - ynfC HEPCGN_06165 0.90 -1.0
1,109,728 + -0.2
1,109,766 - +0.1
1,109,768 + -0.4
1,109,782 - -0.5
1,109,787 + -0.2
1,109,788 - +0.3
1,109,788 - +0.6
1,109,856 - speG HEPCGN_06170 0.12 -1.5
1,109,876 - speG HEPCGN_06170 0.16 +0.5
1,109,890 + speG HEPCGN_06170 0.19 -0.5
1,109,993 - speG HEPCGN_06170 0.37 -0.2
1,109,995 - speG HEPCGN_06170 0.37 -0.2
1,110,287 + speG HEPCGN_06170 0.89 -1.4
1,110,288 - speG HEPCGN_06170 0.89 -1.0
1,110,292 - -2.2
1,110,296 + +1.5
1,110,320 + -2.2
1,110,358 - +0.5
1,110,382 + -0.3
1,110,428 + ynfB HEPCGN_06175 0.14 -1.3
1,110,736 + +0.5
1,110,747 - +0.4
1,110,758 + -0.0

Or see this region's nucleotide sequence