Strain Fitness in Escherichia coli ECOR38 around HEPCGN_06030

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfumC and tus overlap by 4 nucleotidestus and rstB are separated by 75 nucleotides HEPCGN_06025: fumC - class II fumarate hydratase, at 1,079,963 to 1,081,366 fumC HEPCGN_06030: tus - DNA replication terminus site-binding protein, at 1,081,363 to 1,082,292 tus HEPCGN_06035: rstB - two-component system sensor histidine kinase RstB, at 1,082,368 to 1,083,669 rstB Position (kb) 1081 1082 1083Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1080.604 kb on + strand, within fumCat 1080.604 kb on + strand, within fumCat 1080.612 kb on - strand, within fumCat 1080.627 kb on + strand, within fumCat 1080.669 kb on + strand, within fumCat 1080.673 kb on + strand, within fumCat 1080.673 kb on + strand, within fumCat 1080.674 kb on - strand, within fumCat 1080.686 kb on + strand, within fumCat 1080.687 kb on - strand, within fumCat 1081.266 kb on + strandat 1081.267 kb on - strandat 1081.348 kb on + strandat 1081.376 kb on + strandat 1081.376 kb on + strandat 1081.426 kb on - strandat 1081.426 kb on - strandat 1081.428 kb on - strandat 1081.428 kb on - strandat 1081.764 kb on + strand, within tusat 1081.765 kb on - strand, within tusat 1081.765 kb on - strand, within tusat 1082.063 kb on - strand, within tusat 1082.128 kb on - strand, within tusat 1082.142 kb on + strand, within tusat 1082.295 kb on + strandat 1082.296 kb on - strandat 1082.298 kb on + strandat 1082.363 kb on + strandat 1082.364 kb on - strandat 1082.395 kb on - strandat 1082.413 kb on - strandat 1082.413 kb on - strandat 1082.480 kb on - strandat 1082.482 kb on - strandat 1082.565 kb on + strand, within rstBat 1082.565 kb on + strand, within rstBat 1082.566 kb on - strand, within rstBat 1082.614 kb on + strand, within rstBat 1082.614 kb on + strand, within rstBat 1082.615 kb on - strand, within rstBat 1082.638 kb on + strand, within rstBat 1082.877 kb on - strand, within rstBat 1082.897 kb on - strand, within rstBat 1083.024 kb on + strand, within rstBat 1083.025 kb on - strand, within rstBat 1083.076 kb on - strand, within rstBat 1083.124 kb on - strand, within rstBat 1083.176 kb on - strand, within rstBat 1083.180 kb on + strand, within rstBat 1083.180 kb on + strand, within rstBat 1083.181 kb on - strand, within rstBat 1083.232 kb on - strand, within rstBat 1083.232 kb on - strand, within rstBat 1083.232 kb on - strand, within rstBat 1083.285 kb on - strand, within rstBat 1083.285 kb on - strand, within rstBat 1083.291 kb on - strand, within rstB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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1,080,604 + fumC HEPCGN_06025 0.46 -0.5
1,080,604 + fumC HEPCGN_06025 0.46 -1.2
1,080,612 - fumC HEPCGN_06025 0.46 +1.0
1,080,627 + fumC HEPCGN_06025 0.47 +0.6
1,080,669 + fumC HEPCGN_06025 0.50 -0.6
1,080,673 + fumC HEPCGN_06025 0.51 -1.8
1,080,673 + fumC HEPCGN_06025 0.51 +0.5
1,080,674 - fumC HEPCGN_06025 0.51 -0.6
1,080,686 + fumC HEPCGN_06025 0.51 -0.5
1,080,687 - fumC HEPCGN_06025 0.52 -2.6
1,081,266 + +0.7
1,081,267 - +0.7
1,081,348 + -0.5
1,081,376 + -2.0
1,081,376 + -0.5
1,081,426 - +1.5
1,081,426 - -0.6
1,081,428 - -1.0
1,081,428 - +0.2
1,081,764 + tus HEPCGN_06030 0.43 -1.6
1,081,765 - tus HEPCGN_06030 0.43 -1.6
1,081,765 - tus HEPCGN_06030 0.43 -1.9
1,082,063 - tus HEPCGN_06030 0.75 -0.8
1,082,128 - tus HEPCGN_06030 0.82 -0.5
1,082,142 + tus HEPCGN_06030 0.84 -0.5
1,082,295 + +0.0
1,082,296 - -0.1
1,082,298 + +0.7
1,082,363 + -0.9
1,082,364 - +0.8
1,082,395 - -1.3
1,082,413 - +0.9
1,082,413 - +1.4
1,082,480 - +3.0
1,082,482 - +0.5
1,082,565 + rstB HEPCGN_06035 0.15 -1.6
1,082,565 + rstB HEPCGN_06035 0.15 +1.7
1,082,566 - rstB HEPCGN_06035 0.15 +0.4
1,082,614 + rstB HEPCGN_06035 0.19 -0.5
1,082,614 + rstB HEPCGN_06035 0.19 -2.0
1,082,615 - rstB HEPCGN_06035 0.19 -0.8
1,082,638 + rstB HEPCGN_06035 0.21 -1.5
1,082,877 - rstB HEPCGN_06035 0.39 -1.7
1,082,897 - rstB HEPCGN_06035 0.41 +0.4
1,083,024 + rstB HEPCGN_06035 0.50 +0.1
1,083,025 - rstB HEPCGN_06035 0.50 -0.1
1,083,076 - rstB HEPCGN_06035 0.54 -0.3
1,083,124 - rstB HEPCGN_06035 0.58 +0.5
1,083,176 - rstB HEPCGN_06035 0.62 +0.4
1,083,180 + rstB HEPCGN_06035 0.62 +0.2
1,083,180 + rstB HEPCGN_06035 0.62 +2.0
1,083,181 - rstB HEPCGN_06035 0.62 +0.4
1,083,232 - rstB HEPCGN_06035 0.66 -0.5
1,083,232 - rstB HEPCGN_06035 0.66 -1.0
1,083,232 - rstB HEPCGN_06035 0.66 -0.0
1,083,285 - rstB HEPCGN_06035 0.70 -0.5
1,083,285 - rstB HEPCGN_06035 0.70 +2.0
1,083,291 - rstB HEPCGN_06035 0.71 +0.2

Or see this region's nucleotide sequence