Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fumC and tus overlap by 4 nucleotides tus and rstB are separated by 75 nucleotides
HEPCGN_06025: fumC - class II fumarate hydratase, at 1,079,963 to 1,081,366
fumC
HEPCGN_06030: tus - DNA replication terminus site-binding protein, at 1,081,363 to 1,082,292
tus
HEPCGN_06035: rstB - two-component system sensor histidine kinase RstB, at 1,082,368 to 1,083,669
rstB
Position (kb)
1081
1082
1083 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1080.604 kb on + strand, within fumC at 1080.604 kb on + strand, within fumC at 1080.612 kb on - strand, within fumC at 1080.627 kb on + strand, within fumC at 1080.669 kb on + strand, within fumC at 1080.673 kb on + strand, within fumC at 1080.673 kb on + strand, within fumC at 1080.674 kb on - strand, within fumC at 1080.686 kb on + strand, within fumC at 1080.687 kb on - strand, within fumC at 1081.266 kb on + strand at 1081.267 kb on - strand at 1081.348 kb on + strand at 1081.376 kb on + strand at 1081.376 kb on + strand at 1081.426 kb on - strand at 1081.426 kb on - strand at 1081.428 kb on - strand at 1081.428 kb on - strand at 1081.764 kb on + strand, within tus at 1081.765 kb on - strand, within tus at 1081.765 kb on - strand, within tus at 1082.063 kb on - strand, within tus at 1082.128 kb on - strand, within tus at 1082.142 kb on + strand, within tus at 1082.295 kb on + strand at 1082.296 kb on - strand at 1082.298 kb on + strand at 1082.363 kb on + strand at 1082.364 kb on - strand at 1082.395 kb on - strand at 1082.413 kb on - strand at 1082.413 kb on - strand at 1082.480 kb on - strand at 1082.482 kb on - strand at 1082.565 kb on + strand, within rstB at 1082.565 kb on + strand, within rstB at 1082.566 kb on - strand, within rstB at 1082.614 kb on + strand, within rstB at 1082.614 kb on + strand, within rstB at 1082.615 kb on - strand, within rstB at 1082.638 kb on + strand, within rstB at 1082.877 kb on - strand, within rstB at 1082.897 kb on - strand, within rstB at 1083.024 kb on + strand, within rstB at 1083.025 kb on - strand, within rstB at 1083.076 kb on - strand, within rstB at 1083.124 kb on - strand, within rstB at 1083.176 kb on - strand, within rstB at 1083.180 kb on + strand, within rstB at 1083.180 kb on + strand, within rstB at 1083.181 kb on - strand, within rstB at 1083.232 kb on - strand, within rstB at 1083.232 kb on - strand, within rstB at 1083.232 kb on - strand, within rstB at 1083.285 kb on - strand, within rstB at 1083.285 kb on - strand, within rstB at 1083.291 kb on - strand, within rstB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,080,604 + fumC HEPCGN_06025 0.46 -0.5 1,080,604 + fumC HEPCGN_06025 0.46 -1.2 1,080,612 - fumC HEPCGN_06025 0.46 +1.0 1,080,627 + fumC HEPCGN_06025 0.47 +0.6 1,080,669 + fumC HEPCGN_06025 0.50 -0.6 1,080,673 + fumC HEPCGN_06025 0.51 -1.8 1,080,673 + fumC HEPCGN_06025 0.51 +0.5 1,080,674 - fumC HEPCGN_06025 0.51 -0.6 1,080,686 + fumC HEPCGN_06025 0.51 -0.5 1,080,687 - fumC HEPCGN_06025 0.52 -2.6 1,081,266 + +0.7 1,081,267 - +0.7 1,081,348 + -0.5 1,081,376 + -2.0 1,081,376 + -0.5 1,081,426 - +1.5 1,081,426 - -0.6 1,081,428 - -1.0 1,081,428 - +0.2 1,081,764 + tus HEPCGN_06030 0.43 -1.6 1,081,765 - tus HEPCGN_06030 0.43 -1.6 1,081,765 - tus HEPCGN_06030 0.43 -1.9 1,082,063 - tus HEPCGN_06030 0.75 -0.8 1,082,128 - tus HEPCGN_06030 0.82 -0.5 1,082,142 + tus HEPCGN_06030 0.84 -0.5 1,082,295 + +0.0 1,082,296 - -0.1 1,082,298 + +0.7 1,082,363 + -0.9 1,082,364 - +0.8 1,082,395 - -1.3 1,082,413 - +0.9 1,082,413 - +1.4 1,082,480 - +3.0 1,082,482 - +0.5 1,082,565 + rstB HEPCGN_06035 0.15 -1.6 1,082,565 + rstB HEPCGN_06035 0.15 +1.7 1,082,566 - rstB HEPCGN_06035 0.15 +0.4 1,082,614 + rstB HEPCGN_06035 0.19 -0.5 1,082,614 + rstB HEPCGN_06035 0.19 -2.0 1,082,615 - rstB HEPCGN_06035 0.19 -0.8 1,082,638 + rstB HEPCGN_06035 0.21 -1.5 1,082,877 - rstB HEPCGN_06035 0.39 -1.7 1,082,897 - rstB HEPCGN_06035 0.41 +0.4 1,083,024 + rstB HEPCGN_06035 0.50 +0.1 1,083,025 - rstB HEPCGN_06035 0.50 -0.1 1,083,076 - rstB HEPCGN_06035 0.54 -0.3 1,083,124 - rstB HEPCGN_06035 0.58 +0.5 1,083,176 - rstB HEPCGN_06035 0.62 +0.4 1,083,180 + rstB HEPCGN_06035 0.62 +0.2 1,083,180 + rstB HEPCGN_06035 0.62 +2.0 1,083,181 - rstB HEPCGN_06035 0.62 +0.4 1,083,232 - rstB HEPCGN_06035 0.66 -0.5 1,083,232 - rstB HEPCGN_06035 0.66 -1.0 1,083,232 - rstB HEPCGN_06035 0.66 -0.0 1,083,285 - rstB HEPCGN_06035 0.70 -0.5 1,083,285 - rstB HEPCGN_06035 0.70 +2.0 1,083,291 - rstB HEPCGN_06035 0.71 +0.2
Or see this region's nucleotide sequence