Strain Fitness in Escherichia coli ECOR38 around HEPCGN_05985

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHEPCGN_05980 and hdhA are separated by 61 nucleotideshdhA and uidR are separated by 232 nucleotides HEPCGN_05980: HEPCGN_05980 - IS4-like element IS4 family transposase, at 1,067,301 to 1,068,629 _05980 HEPCGN_05985: hdhA - 7-alpha-hydroxysteroid dehydrogenase, at 1,068,691 to 1,069,458 hdhA HEPCGN_05990: uidR - DNA-binding transcriptional regulator UidR, at 1,069,691 to 1,070,269 uidR Position (kb) 1068 1069 1070Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1068.756 kb on + strandat 1068.819 kb on + strand, within hdhAat 1068.834 kb on + strand, within hdhAat 1068.835 kb on - strand, within hdhAat 1068.897 kb on + strand, within hdhAat 1069.266 kb on - strand, within hdhAat 1069.486 kb on - strandat 1069.486 kb on - strandat 1069.525 kb on + strandat 1069.580 kb on + strandat 1069.580 kb on + strandat 1069.623 kb on - strandat 1069.646 kb on - strandat 1069.663 kb on + strandat 1069.667 kb on + strandat 1069.667 kb on + strandat 1069.668 kb on - strandat 1069.668 kb on - strandat 1069.668 kb on - strandat 1069.668 kb on - strandat 1069.678 kb on + strandat 1069.679 kb on - strandat 1069.679 kb on - strandat 1069.823 kb on + strand, within uidRat 1069.865 kb on - strand, within uidRat 1069.934 kb on + strand, within uidRat 1069.934 kb on + strand, within uidRat 1069.934 kb on + strand, within uidRat 1069.935 kb on - strand, within uidRat 1069.935 kb on - strand, within uidRat 1069.936 kb on + strand, within uidRat 1069.937 kb on - strand, within uidRat 1070.050 kb on + strand, within uidRat 1070.051 kb on - strand, within uidRat 1070.115 kb on + strand, within uidRat 1070.115 kb on + strand, within uidRat 1070.169 kb on - strand, within uidRat 1070.233 kb on - strandat 1070.251 kb on + strandat 1070.253 kb on + strandat 1070.318 kb on - strandat 1070.322 kb on + strandat 1070.393 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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1,068,756 + -1.0
1,068,819 + hdhA HEPCGN_05985 0.17 +1.0
1,068,834 + hdhA HEPCGN_05985 0.19 +2.6
1,068,835 - hdhA HEPCGN_05985 0.19 +1.8
1,068,897 + hdhA HEPCGN_05985 0.27 +0.0
1,069,266 - hdhA HEPCGN_05985 0.75 -0.5
1,069,486 - +0.5
1,069,486 - -0.8
1,069,525 + +1.4
1,069,580 + -1.9
1,069,580 + +0.5
1,069,623 - -1.1
1,069,646 - -1.4
1,069,663 + +2.2
1,069,667 + +0.5
1,069,667 + +0.3
1,069,668 - +2.0
1,069,668 - -0.4
1,069,668 - +0.5
1,069,668 - -0.8
1,069,678 + +1.7
1,069,679 - -1.0
1,069,679 - -0.8
1,069,823 + uidR HEPCGN_05990 0.23 -0.2
1,069,865 - uidR HEPCGN_05990 0.30 +0.3
1,069,934 + uidR HEPCGN_05990 0.42 -0.5
1,069,934 + uidR HEPCGN_05990 0.42 -1.3
1,069,934 + uidR HEPCGN_05990 0.42 -0.1
1,069,935 - uidR HEPCGN_05990 0.42 +1.3
1,069,935 - uidR HEPCGN_05990 0.42 -2.9
1,069,936 + uidR HEPCGN_05990 0.42 -2.4
1,069,937 - uidR HEPCGN_05990 0.42 +1.2
1,070,050 + uidR HEPCGN_05990 0.62 -1.0
1,070,051 - uidR HEPCGN_05990 0.62 -0.4
1,070,115 + uidR HEPCGN_05990 0.73 +0.4
1,070,115 + uidR HEPCGN_05990 0.73 +0.2
1,070,169 - uidR HEPCGN_05990 0.83 +2.8
1,070,233 - +0.5
1,070,251 + -1.4
1,070,253 + -1.7
1,070,318 - +0.3
1,070,322 + -0.4
1,070,393 - +2.5

Or see this region's nucleotide sequence