Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhS and ydhR are separated by 125 nucleotides ydhR and Val_trna are separated by 108 nucleotides Val_trna and Val_trna are separated by 4 nucleotides Val_trna and ydhQ are separated by 307 nucleotides
HEPCGN_05715: ydhS - FAD-NAD(P)-binding protein, at 1,016,559 to 1,018,163
ydhS
HEPCGN_05720: ydhR - monooxygenase, at 1,018,289 to 1,018,594
ydhR
HEPCGN_05730: Val_trna - tRNA-Val(gac), at 1,018,703 to 1,018,779
_trna
HEPCGN_05735: Val_trna - tRNA-Val(gac), at 1,018,784 to 1,018,860
_trna
HEPCGN_05740: ydhQ - Uncharacterized protein YdhQ, at 1,019,168 to 1,020,424
ydhQ
Position (kb)
1018
1019 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1017.300 kb on + strand, within ydhS at 1017.300 kb on + strand, within ydhS at 1017.300 kb on + strand, within ydhS at 1017.308 kb on + strand, within ydhS at 1017.332 kb on - strand, within ydhS at 1017.357 kb on + strand, within ydhS at 1017.452 kb on + strand, within ydhS at 1017.579 kb on - strand, within ydhS at 1017.637 kb on + strand, within ydhS at 1017.638 kb on - strand, within ydhS at 1017.822 kb on + strand, within ydhS at 1017.913 kb on + strand, within ydhS at 1017.946 kb on - strand, within ydhS at 1017.963 kb on - strand, within ydhS at 1017.987 kb on - strand, within ydhS at 1017.995 kb on + strand, within ydhS at 1018.079 kb on + strand at 1018.101 kb on + strand at 1018.113 kb on + strand at 1018.120 kb on + strand at 1018.167 kb on + strand at 1018.171 kb on + strand at 1018.195 kb on + strand at 1018.208 kb on + strand at 1018.208 kb on + strand at 1018.387 kb on + strand, within ydhR at 1018.426 kb on + strand, within ydhR at 1018.434 kb on + strand, within ydhR at 1018.526 kb on - strand, within ydhR at 1018.581 kb on - strand at 1018.581 kb on - strand at 1018.633 kb on - strand at 1018.693 kb on + strand at 1018.772 kb on - strand at 1019.102 kb on - strand at 1019.125 kb on + strand at 1019.125 kb on + strand at 1019.125 kb on + strand at 1019.126 kb on - strand at 1019.129 kb on + strand at 1019.135 kb on + strand at 1019.163 kb on - strand at 1019.438 kb on - strand, within ydhQ at 1019.446 kb on + strand, within ydhQ at 1019.497 kb on + strand, within ydhQ at 1019.504 kb on + strand, within ydhQ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,017,300 + ydhS HEPCGN_05715 0.46 +0.6 1,017,300 + ydhS HEPCGN_05715 0.46 -1.7 1,017,300 + ydhS HEPCGN_05715 0.46 -1.8 1,017,308 + ydhS HEPCGN_05715 0.47 -0.9 1,017,332 - ydhS HEPCGN_05715 0.48 +0.1 1,017,357 + ydhS HEPCGN_05715 0.50 +0.3 1,017,452 + ydhS HEPCGN_05715 0.56 -0.6 1,017,579 - ydhS HEPCGN_05715 0.64 -1.8 1,017,637 + ydhS HEPCGN_05715 0.67 +0.0 1,017,638 - ydhS HEPCGN_05715 0.67 +1.0 1,017,822 + ydhS HEPCGN_05715 0.79 +2.0 1,017,913 + ydhS HEPCGN_05715 0.84 +0.8 1,017,946 - ydhS HEPCGN_05715 0.86 +0.5 1,017,963 - ydhS HEPCGN_05715 0.87 -1.0 1,017,987 - ydhS HEPCGN_05715 0.89 -0.9 1,017,995 + ydhS HEPCGN_05715 0.89 -2.1 1,018,079 + -0.9 1,018,101 + -1.3 1,018,113 + +2.3 1,018,120 + +0.3 1,018,167 + -1.0 1,018,171 + -0.8 1,018,195 + -1.0 1,018,208 + +0.7 1,018,208 + +2.3 1,018,387 + ydhR HEPCGN_05720 0.32 -0.5 1,018,426 + ydhR HEPCGN_05720 0.45 +1.7 1,018,434 + ydhR HEPCGN_05720 0.47 +1.9 1,018,526 - ydhR HEPCGN_05720 0.77 +0.1 1,018,581 - +0.0 1,018,581 - +0.1 1,018,633 - +0.9 1,018,693 + -1.1 1,018,772 - -1.5 1,019,102 - -0.4 1,019,125 + -0.9 1,019,125 + -0.1 1,019,125 + -0.2 1,019,126 - +2.0 1,019,129 + -0.2 1,019,135 + +0.2 1,019,163 - -1.0 1,019,438 - ydhQ HEPCGN_05740 0.21 +0.2 1,019,446 + ydhQ HEPCGN_05740 0.22 -1.4 1,019,497 + ydhQ HEPCGN_05740 0.26 +1.7 1,019,504 + ydhQ HEPCGN_05740 0.27 -0.4
Or see this region's nucleotide sequence