Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cspE and rlmA are separated by 165 nucleotides rlmA and mntP overlap by 4 nucleotides mntP and HEPCGN_04910 are separated by 428 nucleotides HEPCGN_04910 and manZ are separated by 54 nucleotides
HEPCGN_04895: cspE - transcription antiterminator/RNA stability regulator CspE, at 855,368 to 855,577
cspE
HEPCGN_04900: rlmA - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 855,743 to 856,552
rlmA
HEPCGN_04905: mntP - manganese efflux pump MntP, at 856,549 to 857,115
mntP
HEPCGN_04910: HEPCGN_04910 - UPF0266 membrane protein YobD, at 857,544 to 858,002
_04910
HEPCGN_04915: manZ - PTS mannose transporter subunit IID, at 858,057 to 858,908
manZ
Position (kb)
856
857
858 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 855.571 kb on + strand at 855.604 kb on + strand at 855.623 kb on - strand at 855.722 kb on + strand at 855.797 kb on + strand at 855.798 kb on - strand at 855.798 kb on - strand at 855.799 kb on + strand at 855.799 kb on + strand at 855.827 kb on - strand, within rlmA at 855.842 kb on + strand, within rlmA at 855.843 kb on - strand, within rlmA at 855.843 kb on - strand, within rlmA at 855.927 kb on + strand, within rlmA at 855.927 kb on + strand, within rlmA at 855.928 kb on - strand, within rlmA at 855.942 kb on - strand, within rlmA at 856.038 kb on - strand, within rlmA at 856.102 kb on - strand, within rlmA at 856.201 kb on - strand, within rlmA at 856.201 kb on - strand, within rlmA at 856.204 kb on + strand, within rlmA at 856.205 kb on - strand, within rlmA at 856.220 kb on - strand, within rlmA at 856.220 kb on - strand, within rlmA at 856.326 kb on + strand, within rlmA at 856.326 kb on + strand, within rlmA at 856.371 kb on + strand, within rlmA at 856.547 kb on + strand at 856.548 kb on - strand at 856.678 kb on - strand, within mntP at 856.678 kb on - strand, within mntP at 857.080 kb on - strand at 857.101 kb on - strand at 857.141 kb on + strand at 857.174 kb on - strand at 857.174 kb on - strand at 857.174 kb on - strand at 857.174 kb on - strand at 857.174 kb on - strand at 857.218 kb on - strand at 857.290 kb on - strand at 857.388 kb on - strand at 857.477 kb on + strand at 857.625 kb on + strand, within HEPCGN_04910 at 857.625 kb on + strand, within HEPCGN_04910 at 857.756 kb on - strand, within HEPCGN_04910 at 857.796 kb on + strand, within HEPCGN_04910 at 857.797 kb on - strand, within HEPCGN_04910 at 857.941 kb on + strand, within HEPCGN_04910 at 857.941 kb on + strand, within HEPCGN_04910 at 858.003 kb on + strand at 858.058 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 855,571 + -0.2 855,604 + +0.6 855,623 - +0.4 855,722 + +0.4 855,797 + -0.0 855,798 - -0.5 855,798 - -0.4 855,799 + -0.1 855,799 + -2.6 855,827 - rlmA HEPCGN_04900 0.10 +0.9 855,842 + rlmA HEPCGN_04900 0.12 -0.3 855,843 - rlmA HEPCGN_04900 0.12 +0.2 855,843 - rlmA HEPCGN_04900 0.12 -1.0 855,927 + rlmA HEPCGN_04900 0.23 -0.5 855,927 + rlmA HEPCGN_04900 0.23 -0.7 855,928 - rlmA HEPCGN_04900 0.23 -0.5 855,942 - rlmA HEPCGN_04900 0.25 -0.0 856,038 - rlmA HEPCGN_04900 0.36 +2.1 856,102 - rlmA HEPCGN_04900 0.44 -0.3 856,201 - rlmA HEPCGN_04900 0.57 +0.2 856,201 - rlmA HEPCGN_04900 0.57 +0.7 856,204 + rlmA HEPCGN_04900 0.57 -0.2 856,205 - rlmA HEPCGN_04900 0.57 -2.1 856,220 - rlmA HEPCGN_04900 0.59 -1.7 856,220 - rlmA HEPCGN_04900 0.59 +0.7 856,326 + rlmA HEPCGN_04900 0.72 -1.6 856,326 + rlmA HEPCGN_04900 0.72 +0.7 856,371 + rlmA HEPCGN_04900 0.78 -1.0 856,547 + -0.3 856,548 - +0.8 856,678 - mntP HEPCGN_04905 0.23 -1.6 856,678 - mntP HEPCGN_04905 0.23 +1.6 857,080 - -0.1 857,101 - +0.9 857,141 + -1.6 857,174 - +0.3 857,174 - -0.7 857,174 - +2.1 857,174 - -1.2 857,174 - +0.5 857,218 - -0.2 857,290 - -0.1 857,388 - -0.1 857,477 + +0.3 857,625 + HEPCGN_04910 0.18 +0.2 857,625 + HEPCGN_04910 0.18 -0.0 857,756 - HEPCGN_04910 0.46 -0.9 857,796 + HEPCGN_04910 0.55 -0.4 857,797 - HEPCGN_04910 0.55 +0.6 857,941 + HEPCGN_04910 0.86 +0.3 857,941 + HEPCGN_04910 0.86 +0.1 858,003 + -0.7 858,058 + -0.1
Or see this region's nucleotide sequence