Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purT and yebG are separated by 133 nucleotides yebG and yebF are separated by 66 nucleotides yebF and HEPCGN_04770 are separated by 155 nucleotides HEPCGN_04770 and yebE are separated by 59 nucleotides
HEPCGN_04755: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 830,749 to 831,927
purT
HEPCGN_04760: yebG - DNA damage-inducible protein YebG, at 832,061 to 832,351
yebG
HEPCGN_04765: yebF - Protein YebF, at 832,418 to 832,774
yebF
HEPCGN_04770: HEPCGN_04770 - hypothetical protein, at 832,930 to 833,040
_04770
HEPCGN_04775: yebE - Inner membrane protein YebE, at 833,100 to 833,759
yebE
Position (kb) 832
833 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 831.352 kb on + strand, within purT at 831.352 kb on + strand, within purT at 831.426 kb on + strand, within purT at 831.672 kb on + strand, within purT at 831.673 kb on - strand, within purT at 831.693 kb on + strand, within purT at 831.693 kb on + strand, within purT at 831.696 kb on - strand, within purT at 831.696 kb on - strand, within purT at 831.727 kb on + strand, within purT at 831.750 kb on + strand, within purT at 831.750 kb on + strand, within purT at 831.751 kb on - strand, within purT at 831.780 kb on + strand, within purT at 831.780 kb on + strand, within purT at 831.780 kb on + strand, within purT at 831.875 kb on - strand at 831.878 kb on - strand at 831.878 kb on - strand at 831.919 kb on + strand at 832.079 kb on + strand at 832.079 kb on + strand at 832.142 kb on + strand, within yebG at 832.405 kb on + strand at 832.406 kb on - strand at 832.558 kb on - strand, within yebF at 832.568 kb on + strand, within yebF at 832.672 kb on + strand, within yebF at 832.703 kb on - strand, within yebF at 832.786 kb on - strand at 832.921 kb on + strand at 832.937 kb on - strand at 832.954 kb on + strand, within HEPCGN_04770 at 832.955 kb on - strand, within HEPCGN_04770 at 832.955 kb on - strand, within HEPCGN_04770 at 832.955 kb on - strand, within HEPCGN_04770 at 832.959 kb on + strand, within HEPCGN_04770 at 833.067 kb on - strand at 833.337 kb on - strand, within yebE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 831,352 + purT HEPCGN_04755 0.51 +2.6 831,352 + purT HEPCGN_04755 0.51 -0.7 831,426 + purT HEPCGN_04755 0.57 -2.5 831,672 + purT HEPCGN_04755 0.78 -1.0 831,673 - purT HEPCGN_04755 0.78 -0.1 831,693 + purT HEPCGN_04755 0.80 +0.5 831,693 + purT HEPCGN_04755 0.80 -0.1 831,696 - purT HEPCGN_04755 0.80 -1.0 831,696 - purT HEPCGN_04755 0.80 +0.9 831,727 + purT HEPCGN_04755 0.83 -0.3 831,750 + purT HEPCGN_04755 0.85 -0.9 831,750 + purT HEPCGN_04755 0.85 +1.5 831,751 - purT HEPCGN_04755 0.85 -0.5 831,780 + purT HEPCGN_04755 0.87 +1.4 831,780 + purT HEPCGN_04755 0.87 -0.1 831,780 + purT HEPCGN_04755 0.87 -1.1 831,875 - -0.2 831,878 - -1.2 831,878 - -0.7 831,919 + +0.1 832,079 + -1.0 832,079 + +0.0 832,142 + yebG HEPCGN_04760 0.28 +1.3 832,405 + +0.2 832,406 - -0.4 832,558 - yebF HEPCGN_04765 0.39 -1.6 832,568 + yebF HEPCGN_04765 0.42 +2.5 832,672 + yebF HEPCGN_04765 0.71 -0.3 832,703 - yebF HEPCGN_04765 0.80 +0.4 832,786 - +0.3 832,921 + -0.5 832,937 - -3.4 832,954 + HEPCGN_04770 0.22 +0.3 832,955 - HEPCGN_04770 0.23 +0.2 832,955 - HEPCGN_04770 0.23 -3.1 832,955 - HEPCGN_04770 0.23 +0.6 832,959 + HEPCGN_04770 0.26 +2.3 833,067 - -0.1 833,337 - yebE HEPCGN_04775 0.36 -2.3
Or see this region's nucleotide sequence