Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ile2_trna and Lys_trna are separated by 1 nucleotides Lys_trna and Thr_trna are separated by 2 nucleotides Thr_trna and Gly_trna are separated by 3 nucleotides Gly_trna and HEPCGN_03750 are separated by 292 nucleotides HEPCGN_03750 and HEPCGN_03755 are separated by 4 nucleotides HEPCGN_03755 and HEPCGN_03760 are separated by 63 nucleotides HEPCGN_03760 and HEPCGN_03765 overlap by 4 nucleotides
HEPCGN_03730: Ile2_trna - tRNA-Ile2(cat), at 661,305 to 661,380
_trna
HEPCGN_03735: Lys_trna - tRNA-Lys(ttt), at 661,382 to 661,458
_trna
HEPCGN_03740: Thr_trna - tRNA-Thr(tgt), at 661,461 to 661,536
_trna
HEPCGN_03745: Gly_trna - tRNA-Gly(tcc), at 661,540 to 661,614
_trna
HEPCGN_03750: HEPCGN_03750 - holin, at 661,907 to 662,122
_03750
HEPCGN_03755: HEPCGN_03755 - DUF1327 domain-containing protein, at 662,127 to 662,477
_03755
HEPCGN_03760: HEPCGN_03760 - lysozyme, at 662,541 to 663,074
_03760
HEPCGN_03765: HEPCGN_03765 - lysis protein, at 663,071 to 663,538
_03765
Position (kb) 662
663 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 661.193 kb on + strand at 661.203 kb on - strand at 661.232 kb on + strand at 661.537 kb on + strand at 661.563 kb on - strand, within Gly_trna at 661.563 kb on - strand, within Gly_trna at 661.671 kb on - strand at 661.671 kb on - strand at 661.676 kb on - strand at 661.744 kb on + strand at 661.809 kb on + strand at 661.809 kb on + strand at 661.810 kb on - strand at 661.810 kb on - strand at 661.811 kb on + strand at 661.811 kb on + strand at 661.812 kb on - strand at 661.813 kb on + strand at 662.060 kb on + strand, within HEPCGN_03750 at 662.060 kb on + strand, within HEPCGN_03750 at 662.412 kb on + strand, within HEPCGN_03755 at 662.476 kb on - strand at 662.476 kb on - strand at 662.496 kb on + strand at 662.888 kb on + strand, within HEPCGN_03760 at 662.910 kb on + strand, within HEPCGN_03760 at 662.995 kb on + strand, within HEPCGN_03760 at 662.996 kb on - strand, within HEPCGN_03760 at 663.091 kb on + strand at 663.096 kb on - strand at 663.168 kb on - strand, within HEPCGN_03765
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 661,193 + +0.1 661,203 - -1.4 661,232 + -0.9 661,537 + -0.5 661,563 - Gly_trna HEPCGN_03745 0.31 -0.6 661,563 - Gly_trna HEPCGN_03745 0.31 -1.5 661,671 - -0.8 661,671 - -0.7 661,676 - -2.2 661,744 + -0.4 661,809 + -0.2 661,809 + -1.1 661,810 - +0.4 661,810 - +0.5 661,811 + -1.2 661,811 + +3.1 661,812 - -1.1 661,813 + -0.9 662,060 + HEPCGN_03750 0.71 -0.6 662,060 + HEPCGN_03750 0.71 -0.6 662,412 + HEPCGN_03755 0.81 -1.3 662,476 - +0.2 662,476 - -1.4 662,496 + -0.2 662,888 + HEPCGN_03760 0.65 +0.8 662,910 + HEPCGN_03760 0.69 +0.4 662,995 + HEPCGN_03760 0.85 -0.8 662,996 - HEPCGN_03760 0.85 -1.9 663,091 + -0.1 663,096 - -1.2 663,168 - HEPCGN_03765 0.21 +0.1
Or see this region's nucleotide sequence