Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzzB and hisIE are separated by 95 nucleotides hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides
HEPCGN_03185: wzzB - LPS O-antigen chain length determinant protein WzzB, at 549,781 to 550,761
wzzB
HEPCGN_03190: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 550,857 to 551,468
hisIE
HEPCGN_03195: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 551,462 to 552,238
hisF
HEPCGN_03200: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 552,220 to 552,957
hisA
Position (kb) 550
551
552 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 549.915 kb on + strand, within wzzB at 550.017 kb on + strand, within wzzB at 550.017 kb on + strand, within wzzB at 550.021 kb on + strand, within wzzB at 550.155 kb on + strand, within wzzB at 550.268 kb on + strand, within wzzB at 550.268 kb on + strand, within wzzB at 550.461 kb on + strand, within wzzB at 550.543 kb on + strand, within wzzB at 550.546 kb on + strand, within wzzB at 550.546 kb on + strand, within wzzB at 550.583 kb on + strand, within wzzB at 550.622 kb on + strand, within wzzB at 550.748 kb on - strand at 550.759 kb on + strand at 550.936 kb on + strand, within hisIE at 551.068 kb on + strand, within hisIE at 551.068 kb on + strand, within hisIE at 551.248 kb on - strand, within hisIE at 551.298 kb on - strand, within hisIE at 551.360 kb on + strand, within hisIE at 551.361 kb on - strand, within hisIE at 551.361 kb on - strand, within hisIE at 551.362 kb on + strand, within hisIE at 551.388 kb on + strand, within hisIE at 551.414 kb on + strand at 551.468 kb on + strand at 551.468 kb on + strand at 551.469 kb on - strand at 551.500 kb on - strand at 551.541 kb on - strand, within hisF at 551.707 kb on + strand, within hisF at 551.803 kb on + strand, within hisF at 551.821 kb on - strand, within hisF at 551.841 kb on + strand, within hisF at 551.866 kb on + strand, within hisF at 551.866 kb on + strand, within hisF at 551.867 kb on - strand, within hisF at 551.867 kb on - strand, within hisF at 551.990 kb on - strand, within hisF at 552.053 kb on - strand, within hisF at 552.056 kb on + strand, within hisF at 552.057 kb on - strand, within hisF at 552.090 kb on + strand, within hisF at 552.140 kb on + strand, within hisF at 552.176 kb on + strand at 552.289 kb on + strand at 552.398 kb on + strand, within hisA at 552.400 kb on + strand, within hisA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 549,915 + wzzB HEPCGN_03185 0.14 -1.9 550,017 + wzzB HEPCGN_03185 0.24 -2.0 550,017 + wzzB HEPCGN_03185 0.24 -0.7 550,021 + wzzB HEPCGN_03185 0.24 -1.7 550,155 + wzzB HEPCGN_03185 0.38 -0.4 550,268 + wzzB HEPCGN_03185 0.50 -0.5 550,268 + wzzB HEPCGN_03185 0.50 -2.6 550,461 + wzzB HEPCGN_03185 0.69 -0.3 550,543 + wzzB HEPCGN_03185 0.78 -0.2 550,546 + wzzB HEPCGN_03185 0.78 -0.5 550,546 + wzzB HEPCGN_03185 0.78 -0.6 550,583 + wzzB HEPCGN_03185 0.82 +2.1 550,622 + wzzB HEPCGN_03185 0.86 -2.1 550,748 - +1.2 550,759 + -0.4 550,936 + hisIE HEPCGN_03190 0.13 +0.2 551,068 + hisIE HEPCGN_03190 0.34 +0.6 551,068 + hisIE HEPCGN_03190 0.34 +1.0 551,248 - hisIE HEPCGN_03190 0.64 -0.4 551,298 - hisIE HEPCGN_03190 0.72 +2.1 551,360 + hisIE HEPCGN_03190 0.82 +0.5 551,361 - hisIE HEPCGN_03190 0.82 +1.0 551,361 - hisIE HEPCGN_03190 0.82 -0.6 551,362 + hisIE HEPCGN_03190 0.83 +1.6 551,388 + hisIE HEPCGN_03190 0.87 +3.1 551,414 + +1.3 551,468 + -0.8 551,468 + -1.3 551,469 - +0.5 551,500 - -0.7 551,541 - hisF HEPCGN_03195 0.10 -2.0 551,707 + hisF HEPCGN_03195 0.32 -0.7 551,803 + hisF HEPCGN_03195 0.44 +0.6 551,821 - hisF HEPCGN_03195 0.46 -0.9 551,841 + hisF HEPCGN_03195 0.49 -0.3 551,866 + hisF HEPCGN_03195 0.52 +0.6 551,866 + hisF HEPCGN_03195 0.52 -0.5 551,867 - hisF HEPCGN_03195 0.52 +1.1 551,867 - hisF HEPCGN_03195 0.52 -1.0 551,990 - hisF HEPCGN_03195 0.68 -0.0 552,053 - hisF HEPCGN_03195 0.76 -0.6 552,056 + hisF HEPCGN_03195 0.76 -0.5 552,057 - hisF HEPCGN_03195 0.77 +0.5 552,090 + hisF HEPCGN_03195 0.81 +2.5 552,140 + hisF HEPCGN_03195 0.87 +2.2 552,176 + +0.8 552,289 + +0.2 552,398 + hisA HEPCGN_03200 0.24 +1.9 552,400 + hisA HEPCGN_03200 0.24 -0.2
Or see this region's nucleotide sequence