Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ugd and wzzB are separated by 145 nucleotides wzzB and hisIE are separated by 95 nucleotides hisIE and hisF overlap by 7 nucleotides
HEPCGN_03180: ugd - UDP-glucose 6-dehydrogenase, at 548,469 to 549,635
ugd
HEPCGN_03185: wzzB - LPS O-antigen chain length determinant protein WzzB, at 549,781 to 550,761
wzzB
HEPCGN_03190: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 550,857 to 551,468
hisIE
HEPCGN_03195: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 551,462 to 552,238
hisF
Position (kb) 549
550
551 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 548.990 kb on - strand, within ugd at 549.014 kb on + strand, within ugd at 549.025 kb on + strand, within ugd at 549.076 kb on - strand, within ugd at 549.092 kb on - strand, within ugd at 549.101 kb on + strand, within ugd at 549.141 kb on - strand, within ugd at 549.192 kb on + strand, within ugd at 549.240 kb on - strand, within ugd at 549.317 kb on + strand, within ugd at 549.401 kb on + strand, within ugd at 549.471 kb on + strand, within ugd at 549.472 kb on - strand, within ugd at 549.557 kb on + strand at 549.600 kb on - strand at 549.600 kb on - strand at 549.697 kb on + strand at 549.715 kb on + strand at 549.717 kb on + strand at 549.848 kb on + strand at 549.915 kb on + strand, within wzzB at 550.017 kb on + strand, within wzzB at 550.017 kb on + strand, within wzzB at 550.021 kb on + strand, within wzzB at 550.155 kb on + strand, within wzzB at 550.268 kb on + strand, within wzzB at 550.268 kb on + strand, within wzzB at 550.461 kb on + strand, within wzzB at 550.543 kb on + strand, within wzzB at 550.546 kb on + strand, within wzzB at 550.546 kb on + strand, within wzzB at 550.583 kb on + strand, within wzzB at 550.622 kb on + strand, within wzzB at 550.748 kb on - strand at 550.759 kb on + strand at 550.936 kb on + strand, within hisIE at 551.068 kb on + strand, within hisIE at 551.068 kb on + strand, within hisIE at 551.248 kb on - strand, within hisIE at 551.298 kb on - strand, within hisIE at 551.360 kb on + strand, within hisIE at 551.361 kb on - strand, within hisIE at 551.361 kb on - strand, within hisIE at 551.362 kb on + strand, within hisIE at 551.388 kb on + strand, within hisIE at 551.414 kb on + strand at 551.468 kb on + strand at 551.468 kb on + strand at 551.469 kb on - strand at 551.500 kb on - strand at 551.541 kb on - strand, within hisF at 551.707 kb on + strand, within hisF
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 548,990 - ugd HEPCGN_03180 0.45 +4.9 549,014 + ugd HEPCGN_03180 0.47 -0.4 549,025 + ugd HEPCGN_03180 0.48 -0.3 549,076 - ugd HEPCGN_03180 0.52 -0.5 549,092 - ugd HEPCGN_03180 0.53 +0.0 549,101 + ugd HEPCGN_03180 0.54 -0.1 549,141 - ugd HEPCGN_03180 0.58 +0.3 549,192 + ugd HEPCGN_03180 0.62 -1.8 549,240 - ugd HEPCGN_03180 0.66 +0.0 549,317 + ugd HEPCGN_03180 0.73 -0.9 549,401 + ugd HEPCGN_03180 0.80 -0.5 549,471 + ugd HEPCGN_03180 0.86 +0.4 549,472 - ugd HEPCGN_03180 0.86 +0.0 549,557 + -0.3 549,600 - -0.9 549,600 - -0.2 549,697 + -0.5 549,715 + +0.6 549,717 + +2.1 549,848 + -1.0 549,915 + wzzB HEPCGN_03185 0.14 -1.9 550,017 + wzzB HEPCGN_03185 0.24 -2.0 550,017 + wzzB HEPCGN_03185 0.24 -0.7 550,021 + wzzB HEPCGN_03185 0.24 -1.7 550,155 + wzzB HEPCGN_03185 0.38 -0.4 550,268 + wzzB HEPCGN_03185 0.50 -0.5 550,268 + wzzB HEPCGN_03185 0.50 -2.6 550,461 + wzzB HEPCGN_03185 0.69 -0.3 550,543 + wzzB HEPCGN_03185 0.78 -0.2 550,546 + wzzB HEPCGN_03185 0.78 -0.5 550,546 + wzzB HEPCGN_03185 0.78 -0.6 550,583 + wzzB HEPCGN_03185 0.82 +2.1 550,622 + wzzB HEPCGN_03185 0.86 -2.1 550,748 - +1.2 550,759 + -0.4 550,936 + hisIE HEPCGN_03190 0.13 +0.2 551,068 + hisIE HEPCGN_03190 0.34 +0.6 551,068 + hisIE HEPCGN_03190 0.34 +1.0 551,248 - hisIE HEPCGN_03190 0.64 -0.4 551,298 - hisIE HEPCGN_03190 0.72 +2.1 551,360 + hisIE HEPCGN_03190 0.82 +0.5 551,361 - hisIE HEPCGN_03190 0.82 +1.0 551,361 - hisIE HEPCGN_03190 0.82 -0.6 551,362 + hisIE HEPCGN_03190 0.83 +1.6 551,388 + hisIE HEPCGN_03190 0.87 +3.1 551,414 + +1.3 551,468 + -0.8 551,468 + -1.3 551,469 - +0.5 551,500 - -0.7 551,541 - hisF HEPCGN_03195 0.10 -2.0 551,707 + hisF HEPCGN_03195 0.32 -0.7
Or see this region's nucleotide sequence