Strain Fitness in Escherichia coli ECOR38 around HEPCGN_03030

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaC and wcaD overlap by 26 nucleotideswcaD and wcaE are separated by 10 nucleotideswcaE and wcaF are separated by 15 nucleotides HEPCGN_03025: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 513,766 to 514,983 wcaC HEPCGN_03030: wcaD - putative colanic acid polymerase WcaD, at 514,958 to 516,175 wcaD HEPCGN_03035: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 516,186 to 516,932 wcaE HEPCGN_03040: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 516,948 to 517,496 wcaF Position (kb) 514 515 516 517Strain fitness (log2 ratio) -3 -2 -1 0 1at 514.021 kb on + strand, within wcaCat 514.255 kb on - strand, within wcaCat 514.348 kb on - strand, within wcaCat 514.390 kb on + strand, within wcaCat 514.426 kb on - strand, within wcaCat 514.590 kb on + strand, within wcaCat 514.590 kb on + strand, within wcaCat 514.590 kb on + strand, within wcaCat 514.591 kb on - strand, within wcaCat 514.623 kb on + strand, within wcaCat 514.720 kb on + strand, within wcaCat 514.883 kb on + strandat 514.957 kb on + strandat 514.981 kb on + strandat 514.986 kb on - strandat 515.006 kb on + strandat 515.028 kb on + strandat 515.161 kb on + strand, within wcaDat 515.194 kb on + strand, within wcaDat 515.199 kb on - strand, within wcaDat 515.239 kb on + strand, within wcaDat 515.240 kb on - strand, within wcaDat 515.293 kb on + strand, within wcaDat 515.313 kb on - strand, within wcaDat 515.387 kb on - strand, within wcaDat 515.389 kb on + strand, within wcaDat 515.429 kb on + strand, within wcaDat 515.566 kb on + strand, within wcaDat 515.880 kb on - strand, within wcaDat 516.180 kb on - strandat 516.194 kb on + strandat 516.195 kb on - strandat 516.353 kb on + strand, within wcaEat 516.365 kb on - strand, within wcaEat 516.365 kb on - strand, within wcaEat 516.600 kb on - strand, within wcaEat 517.039 kb on + strand, within wcaF

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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514,021 + wcaC HEPCGN_03025 0.21 +0.3
514,255 - wcaC HEPCGN_03025 0.40 +0.4
514,348 - wcaC HEPCGN_03025 0.48 -1.7
514,390 + wcaC HEPCGN_03025 0.51 +1.1
514,426 - wcaC HEPCGN_03025 0.54 -1.4
514,590 + wcaC HEPCGN_03025 0.68 -0.7
514,590 + wcaC HEPCGN_03025 0.68 -0.2
514,590 + wcaC HEPCGN_03025 0.68 -0.3
514,591 - wcaC HEPCGN_03025 0.68 -0.5
514,623 + wcaC HEPCGN_03025 0.70 +1.3
514,720 + wcaC HEPCGN_03025 0.78 -0.1
514,883 + -1.6
514,957 + +0.7
514,981 + -0.8
514,986 - -1.2
515,006 + +1.0
515,028 + -0.8
515,161 + wcaD HEPCGN_03030 0.17 -0.8
515,194 + wcaD HEPCGN_03030 0.19 +0.1
515,199 - wcaD HEPCGN_03030 0.20 -3.2
515,239 + wcaD HEPCGN_03030 0.23 -2.2
515,240 - wcaD HEPCGN_03030 0.23 -3.0
515,293 + wcaD HEPCGN_03030 0.28 -2.3
515,313 - wcaD HEPCGN_03030 0.29 +0.3
515,387 - wcaD HEPCGN_03030 0.35 -1.7
515,389 + wcaD HEPCGN_03030 0.35 -0.2
515,429 + wcaD HEPCGN_03030 0.39 -0.6
515,566 + wcaD HEPCGN_03030 0.50 -1.1
515,880 - wcaD HEPCGN_03030 0.76 -1.1
516,180 - +0.5
516,194 + -1.0
516,195 - +0.2
516,353 + wcaE HEPCGN_03035 0.22 +0.2
516,365 - wcaE HEPCGN_03035 0.24 +1.5
516,365 - wcaE HEPCGN_03035 0.24 +0.1
516,600 - wcaE HEPCGN_03035 0.55 -0.2
517,039 + wcaF HEPCGN_03040 0.17 +0.1

Or see this region's nucleotide sequence