Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzc and wcaA are separated by 92 nucleotides wcaA and wcaB are separated by 2 nucleotides wcaB and wcaC overlap by 4 nucleotides
HEPCGN_03010: wzc - tyrosine-protein kinase Wzc, at 510,184 to 512,346
wzc
HEPCGN_03015: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 512,439 to 513,278
wcaA
HEPCGN_03020: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 513,281 to 513,769
wcaB
HEPCGN_03025: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 513,766 to 514,983
wcaC
Position (kb) 513
514 Strain fitness (log2 ratio) -2
-1
0
1 at 512.297 kb on - strand at 512.326 kb on + strand at 512.326 kb on + strand at 512.327 kb on - strand at 512.327 kb on - strand at 512.344 kb on + strand at 512.438 kb on + strand at 512.439 kb on - strand at 512.439 kb on - strand at 512.466 kb on + strand at 512.466 kb on + strand at 512.466 kb on + strand at 512.466 kb on + strand at 512.466 kb on + strand at 512.468 kb on + strand at 512.532 kb on + strand, within wcaA at 512.532 kb on + strand, within wcaA at 512.533 kb on - strand, within wcaA at 512.587 kb on + strand, within wcaA at 512.588 kb on - strand, within wcaA at 512.595 kb on + strand, within wcaA at 512.595 kb on + strand, within wcaA at 512.596 kb on - strand, within wcaA at 512.596 kb on - strand, within wcaA at 512.629 kb on - strand, within wcaA at 512.669 kb on + strand, within wcaA at 512.670 kb on - strand, within wcaA at 512.681 kb on + strand, within wcaA at 512.700 kb on + strand, within wcaA at 512.700 kb on + strand, within wcaA at 512.700 kb on + strand, within wcaA at 512.808 kb on + strand, within wcaA at 512.903 kb on + strand, within wcaA at 513.264 kb on - strand at 513.524 kb on - strand, within wcaB at 513.658 kb on + strand, within wcaB at 513.671 kb on - strand, within wcaB at 513.764 kb on - strand at 513.764 kb on - strand at 513.771 kb on + strand at 513.771 kb on + strand at 513.939 kb on + strand, within wcaC at 514.021 kb on + strand, within wcaC at 514.255 kb on - strand, within wcaC at 514.348 kb on - strand, within wcaC at 514.390 kb on + strand, within wcaC at 514.426 kb on - strand, within wcaC at 514.590 kb on + strand, within wcaC at 514.590 kb on + strand, within wcaC at 514.590 kb on + strand, within wcaC at 514.591 kb on - strand, within wcaC at 514.623 kb on + strand, within wcaC at 514.720 kb on + strand, within wcaC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 512,297 - -0.4 512,326 + -0.4 512,326 + -0.7 512,327 - -0.3 512,327 - -0.9 512,344 + -0.4 512,438 + +0.2 512,439 - +0.6 512,439 - -0.3 512,466 + +0.2 512,466 + -0.2 512,466 + +0.2 512,466 + +1.3 512,466 + +0.5 512,468 + -0.7 512,532 + wcaA HEPCGN_03015 0.11 -2.4 512,532 + wcaA HEPCGN_03015 0.11 +1.4 512,533 - wcaA HEPCGN_03015 0.11 -0.3 512,587 + wcaA HEPCGN_03015 0.18 +0.5 512,588 - wcaA HEPCGN_03015 0.18 +0.3 512,595 + wcaA HEPCGN_03015 0.19 -0.5 512,595 + wcaA HEPCGN_03015 0.19 -0.9 512,596 - wcaA HEPCGN_03015 0.19 +1.5 512,596 - wcaA HEPCGN_03015 0.19 +1.2 512,629 - wcaA HEPCGN_03015 0.23 -0.5 512,669 + wcaA HEPCGN_03015 0.27 +1.0 512,670 - wcaA HEPCGN_03015 0.28 +1.3 512,681 + wcaA HEPCGN_03015 0.29 -0.2 512,700 + wcaA HEPCGN_03015 0.31 +0.3 512,700 + wcaA HEPCGN_03015 0.31 -0.7 512,700 + wcaA HEPCGN_03015 0.31 +0.3 512,808 + wcaA HEPCGN_03015 0.44 +0.9 512,903 + wcaA HEPCGN_03015 0.55 -0.4 513,264 - +1.7 513,524 - wcaB HEPCGN_03020 0.50 +0.5 513,658 + wcaB HEPCGN_03020 0.77 +0.7 513,671 - wcaB HEPCGN_03020 0.80 +1.6 513,764 - +0.5 513,764 - -0.4 513,771 + +0.1 513,771 + -0.0 513,939 + wcaC HEPCGN_03025 0.14 -1.1 514,021 + wcaC HEPCGN_03025 0.21 +0.3 514,255 - wcaC HEPCGN_03025 0.40 +0.4 514,348 - wcaC HEPCGN_03025 0.48 -1.7 514,390 + wcaC HEPCGN_03025 0.51 +1.1 514,426 - wcaC HEPCGN_03025 0.54 -1.4 514,590 + wcaC HEPCGN_03025 0.68 -0.7 514,590 + wcaC HEPCGN_03025 0.68 -0.2 514,590 + wcaC HEPCGN_03025 0.68 -0.3 514,591 - wcaC HEPCGN_03025 0.68 -0.5 514,623 + wcaC HEPCGN_03025 0.70 +1.3 514,720 + wcaC HEPCGN_03025 0.78 -0.1
Or see this region's nucleotide sequence