Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_02905 and HEPCGN_02915 overlap by 35 nucleotides HEPCGN_02915 and HEPCGN_02925 are separated by 271 nucleotides HEPCGN_02925 and ibsA are separated by 272 nucleotides ibsA and terY are separated by 221 nucleotides terY and pphC are separated by 77 nucleotides pphC and yegI overlap by 4 nucleotides
HEPCGN_02905: HEPCGN_02905 - hypothetical protein, at 491,986 to 492,132
_02905
HEPCGN_02915: HEPCGN_02915 - Type I toxin-antitoxin system Ibs family toxin, at 492,098 to 492,151
_02915
HEPCGN_02925: HEPCGN_02925 - Type I toxin-antitoxin system Ibs family toxin, at 492,423 to 492,479
_02925
HEPCGN_02935: ibsA - Small toxic protein IbsA, at 492,752 to 492,811
ibsA
HEPCGN_02940: terY - Uncharacterized protein YegL, at 493,033 to 493,692
terY
HEPCGN_02945: pphC - Serine/threonine-protein phosphatase 3, at 493,770 to 494,450
pphC
HEPCGN_02950: yegI - protein kinase YegI, at 494,447 to 496,387
yegI
Position (kb) 493
494 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 492.132 kb on + strand at 492.150 kb on - strand at 492.198 kb on + strand at 492.454 kb on + strand, within HEPCGN_02925 at 492.484 kb on - strand at 492.565 kb on - strand at 492.771 kb on + strand, within ibsA at 492.781 kb on - strand, within ibsA at 492.781 kb on - strand, within ibsA at 492.809 kb on + strand at 492.810 kb on - strand at 492.944 kb on - strand at 492.944 kb on - strand at 493.009 kb on + strand at 493.167 kb on + strand, within terY at 493.311 kb on + strand, within terY at 493.332 kb on + strand, within terY at 493.347 kb on + strand, within terY at 493.352 kb on + strand, within terY at 493.486 kb on - strand, within terY at 493.486 kb on - strand, within terY at 493.707 kb on + strand at 493.708 kb on - strand at 493.708 kb on - strand at 493.890 kb on + strand, within pphC at 493.890 kb on + strand, within pphC at 493.892 kb on + strand, within pphC at 493.892 kb on + strand, within pphC at 493.892 kb on + strand, within pphC at 493.893 kb on - strand, within pphC at 493.893 kb on - strand, within pphC at 493.893 kb on - strand, within pphC at 493.929 kb on - strand, within pphC at 494.146 kb on - strand, within pphC at 494.146 kb on - strand, within pphC at 494.146 kb on - strand, within pphC at 494.240 kb on + strand, within pphC at 494.353 kb on + strand, within pphC at 494.406 kb on - strand at 494.417 kb on + strand at 494.461 kb on - strand at 494.461 kb on - strand at 494.462 kb on + strand at 494.463 kb on - strand at 494.463 kb on - strand at 494.463 kb on - strand at 494.464 kb on + strand at 494.464 kb on + strand at 494.464 kb on + strand at 494.464 kb on + strand at 494.464 kb on + strand at 494.528 kb on + strand at 494.568 kb on - strand at 494.611 kb on + strand at 494.645 kb on + strand, within yegI
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 492,132 + -2.1 492,150 - +1.2 492,198 + -0.8 492,454 + HEPCGN_02925 0.54 -0.3 492,484 - -0.4 492,565 - +0.9 492,771 + ibsA HEPCGN_02935 0.32 +0.4 492,781 - ibsA HEPCGN_02935 0.48 -0.4 492,781 - ibsA HEPCGN_02935 0.48 -1.4 492,809 + +1.1 492,810 - +2.5 492,944 - -0.6 492,944 - +1.3 493,009 + +0.2 493,167 + terY HEPCGN_02940 0.20 +2.1 493,311 + terY HEPCGN_02940 0.42 -0.1 493,332 + terY HEPCGN_02940 0.45 +0.9 493,347 + terY HEPCGN_02940 0.48 +0.2 493,352 + terY HEPCGN_02940 0.48 +0.8 493,486 - terY HEPCGN_02940 0.69 -0.1 493,486 - terY HEPCGN_02940 0.69 +0.7 493,707 + +0.2 493,708 - +0.8 493,708 - +0.1 493,890 + pphC HEPCGN_02945 0.18 -0.9 493,890 + pphC HEPCGN_02945 0.18 +0.2 493,892 + pphC HEPCGN_02945 0.18 -0.5 493,892 + pphC HEPCGN_02945 0.18 +2.0 493,892 + pphC HEPCGN_02945 0.18 -0.7 493,893 - pphC HEPCGN_02945 0.18 -0.7 493,893 - pphC HEPCGN_02945 0.18 +0.3 493,893 - pphC HEPCGN_02945 0.18 +1.3 493,929 - pphC HEPCGN_02945 0.23 +0.1 494,146 - pphC HEPCGN_02945 0.55 -0.3 494,146 - pphC HEPCGN_02945 0.55 -1.2 494,146 - pphC HEPCGN_02945 0.55 +0.3 494,240 + pphC HEPCGN_02945 0.69 +0.4 494,353 + pphC HEPCGN_02945 0.86 +0.0 494,406 - -1.1 494,417 + -0.3 494,461 - +3.7 494,461 - -2.4 494,462 + +0.5 494,463 - +0.1 494,463 - -0.7 494,463 - +0.8 494,464 + -0.5 494,464 + -1.0 494,464 + +0.6 494,464 + -0.1 494,464 + -0.3 494,528 + +2.1 494,568 - -0.8 494,611 + -1.1 494,645 + yegI HEPCGN_02950 0.10 -1.5
Or see this region's nucleotide sequence