Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrdA and rlmH are separated by 30 nucleotides rlmH and rsfS are separated by 3 nucleotides rsfS and cobC are separated by 259 nucleotides cobC and nadD are separated by 23 nucleotides
HEPCGN_00910: mrdA - peptidoglycan DD-transpeptidase MrdA, at 109,919 to 111,820
mrdA
HEPCGN_00915: rlmH - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 111,851 to 112,318
rlmH
HEPCGN_00920: rsfS - ribosome silencing factor, at 112,322 to 112,639
rsfS
HEPCGN_00925: cobC - adenosylcobalamin/alpha-ribazole phosphatase, at 112,899 to 113,510
cobC
HEPCGN_00930: nadD - nicotinate-nucleotide adenylyltransferase, at 113,534 to 114,175
nadD
Position (kb) 112
113 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 111.850 kb on - strand at 112.068 kb on - strand, within rlmH at 112.496 kb on - strand, within rsfS at 112.685 kb on - strand at 112.904 kb on + strand at 112.905 kb on - strand at 112.905 kb on - strand at 112.905 kb on - strand at 112.942 kb on - strand at 113.009 kb on + strand, within cobC at 113.010 kb on - strand, within cobC at 113.043 kb on + strand, within cobC at 113.056 kb on - strand, within cobC at 113.082 kb on - strand, within cobC at 113.090 kb on - strand, within cobC at 113.105 kb on + strand, within cobC at 113.118 kb on - strand, within cobC at 113.203 kb on + strand, within cobC at 113.203 kb on + strand, within cobC at 113.203 kb on + strand, within cobC at 113.204 kb on - strand, within cobC at 113.358 kb on + strand, within cobC at 113.386 kb on - strand, within cobC at 113.386 kb on - strand, within cobC at 113.386 kb on - strand, within cobC at 113.394 kb on - strand, within cobC at 113.456 kb on - strand at 113.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 111,850 - -1.4 112,068 - rlmH HEPCGN_00915 0.46 +0.9 112,496 - rsfS HEPCGN_00920 0.55 -1.6 112,685 - -0.6 112,904 + -3.0 112,905 - -0.5 112,905 - -0.2 112,905 - +0.1 112,942 - -0.6 113,009 + cobC HEPCGN_00925 0.18 +0.1 113,010 - cobC HEPCGN_00925 0.18 +0.5 113,043 + cobC HEPCGN_00925 0.24 +0.5 113,056 - cobC HEPCGN_00925 0.26 +0.0 113,082 - cobC HEPCGN_00925 0.30 -0.4 113,090 - cobC HEPCGN_00925 0.31 -0.8 113,105 + cobC HEPCGN_00925 0.34 +1.1 113,118 - cobC HEPCGN_00925 0.36 -1.3 113,203 + cobC HEPCGN_00925 0.50 +1.1 113,203 + cobC HEPCGN_00925 0.50 -1.8 113,203 + cobC HEPCGN_00925 0.50 +0.5 113,204 - cobC HEPCGN_00925 0.50 -0.2 113,358 + cobC HEPCGN_00925 0.75 -1.8 113,386 - cobC HEPCGN_00925 0.80 -1.8 113,386 - cobC HEPCGN_00925 0.80 +1.2 113,386 - cobC HEPCGN_00925 0.80 -0.6 113,394 - cobC HEPCGN_00925 0.81 -2.2 113,456 - +0.3 113,541 + -0.1
Or see this region's nucleotide sequence