Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeF and lipB are separated by 258 nucleotides lipB and ybeD are separated by 100 nucleotides ybeD and dacA are separated by 109 nucleotides
HEPCGN_00880: ybeF - YbeF family transcriptional regulator, at 104,027 to 104,980
ybeF
HEPCGN_00885: lipB - lipoyl(octanoyl) transferase LipB, at 105,239 to 105,880
lipB
HEPCGN_00890: ybeD - DUF493 domain-containing protein, at 105,981 to 106,244
ybeD
HEPCGN_00895: dacA - D-alanyl-D-alanine carboxypeptidase DacA, at 106,354 to 107,565
dacA
Position (kb) 105
106 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 104.248 kb on - strand, within ybeF at 104.336 kb on + strand, within ybeF at 104.337 kb on - strand, within ybeF at 104.337 kb on - strand, within ybeF at 104.338 kb on + strand, within ybeF at 104.356 kb on + strand, within ybeF at 104.375 kb on + strand, within ybeF at 104.416 kb on + strand, within ybeF at 104.417 kb on - strand, within ybeF at 104.620 kb on + strand, within ybeF at 104.643 kb on + strand, within ybeF at 104.644 kb on - strand, within ybeF at 104.696 kb on - strand, within ybeF at 104.697 kb on + strand, within ybeF at 104.697 kb on + strand, within ybeF at 104.705 kb on + strand, within ybeF at 104.726 kb on - strand, within ybeF at 104.774 kb on - strand, within ybeF at 104.860 kb on - strand, within ybeF at 104.971 kb on + strand at 105.148 kb on - strand at 105.530 kb on - strand, within lipB at 105.547 kb on + strand, within lipB at 105.624 kb on + strand, within lipB at 105.624 kb on + strand, within lipB at 105.624 kb on + strand, within lipB at 105.652 kb on - strand, within lipB at 105.744 kb on - strand, within lipB at 105.814 kb on + strand, within lipB at 105.835 kb on - strand at 105.923 kb on - strand at 105.924 kb on + strand at 106.082 kb on - strand, within ybeD at 106.118 kb on - strand, within ybeD at 106.268 kb on - strand at 106.272 kb on - strand at 106.313 kb on - strand at 106.313 kb on - strand at 106.313 kb on - strand at 106.316 kb on - strand at 106.316 kb on - strand at 106.316 kb on - strand at 106.317 kb on + strand at 106.333 kb on - strand at 106.384 kb on - strand at 106.544 kb on + strand, within dacA at 106.544 kb on + strand, within dacA at 106.544 kb on + strand, within dacA at 106.544 kb on + strand, within dacA at 106.591 kb on + strand, within dacA at 106.591 kb on + strand, within dacA at 106.591 kb on + strand, within dacA at 106.592 kb on - strand, within dacA at 106.592 kb on - strand, within dacA at 106.609 kb on + strand, within dacA at 106.662 kb on + strand, within dacA at 106.667 kb on - strand, within dacA at 106.814 kb on + strand, within dacA at 106.815 kb on - strand, within dacA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 104,248 - ybeF HEPCGN_00880 0.23 +0.4 104,336 + ybeF HEPCGN_00880 0.32 -1.2 104,337 - ybeF HEPCGN_00880 0.32 -0.7 104,337 - ybeF HEPCGN_00880 0.32 +0.1 104,338 + ybeF HEPCGN_00880 0.33 +0.1 104,356 + ybeF HEPCGN_00880 0.34 +1.1 104,375 + ybeF HEPCGN_00880 0.36 -1.4 104,416 + ybeF HEPCGN_00880 0.41 -1.6 104,417 - ybeF HEPCGN_00880 0.41 -1.3 104,620 + ybeF HEPCGN_00880 0.62 -0.0 104,643 + ybeF HEPCGN_00880 0.65 -1.7 104,644 - ybeF HEPCGN_00880 0.65 +0.3 104,696 - ybeF HEPCGN_00880 0.70 +0.6 104,697 + ybeF HEPCGN_00880 0.70 +0.2 104,697 + ybeF HEPCGN_00880 0.70 -0.1 104,705 + ybeF HEPCGN_00880 0.71 +0.1 104,726 - ybeF HEPCGN_00880 0.73 -2.5 104,774 - ybeF HEPCGN_00880 0.78 +0.7 104,860 - ybeF HEPCGN_00880 0.87 +0.2 104,971 + -0.8 105,148 - +0.2 105,530 - lipB HEPCGN_00885 0.45 +1.3 105,547 + lipB HEPCGN_00885 0.48 -0.5 105,624 + lipB HEPCGN_00885 0.60 +0.5 105,624 + lipB HEPCGN_00885 0.60 +0.1 105,624 + lipB HEPCGN_00885 0.60 -1.0 105,652 - lipB HEPCGN_00885 0.64 -0.4 105,744 - lipB HEPCGN_00885 0.79 -0.2 105,814 + lipB HEPCGN_00885 0.90 +0.5 105,835 - +1.3 105,923 - +0.7 105,924 + +0.2 106,082 - ybeD HEPCGN_00890 0.38 +0.5 106,118 - ybeD HEPCGN_00890 0.52 -0.1 106,268 - -1.6 106,272 - -2.8 106,313 - -0.1 106,313 - +1.8 106,313 - -0.7 106,316 - -0.4 106,316 - +0.3 106,316 - -1.6 106,317 + +4.0 106,333 - -1.6 106,384 - -0.2 106,544 + dacA HEPCGN_00895 0.16 -0.2 106,544 + dacA HEPCGN_00895 0.16 +1.8 106,544 + dacA HEPCGN_00895 0.16 -0.6 106,544 + dacA HEPCGN_00895 0.16 -0.5 106,591 + dacA HEPCGN_00895 0.20 +1.8 106,591 + dacA HEPCGN_00895 0.20 +1.0 106,591 + dacA HEPCGN_00895 0.20 -0.4 106,592 - dacA HEPCGN_00895 0.20 +0.8 106,592 - dacA HEPCGN_00895 0.20 -0.5 106,609 + dacA HEPCGN_00895 0.21 +0.7 106,662 + dacA HEPCGN_00895 0.25 -0.3 106,667 - dacA HEPCGN_00895 0.26 -2.2 106,814 + dacA HEPCGN_00895 0.38 -0.4 106,815 - dacA HEPCGN_00895 0.38 +0.5
Or see this region's nucleotide sequence