Experiment: RB49
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvC and HEPCGN_04680 are separated by 1 nucleotides HEPCGN_04680 and yobI are separated by 70 nucleotides yobI and ruvA are separated by 138 nucleotides ruvA and ruvB are separated by 8 nucleotides
HEPCGN_04675: ruvC - crossover junction endodeoxyribonuclease RuvC, at 815,432 to 815,953
ruvC
HEPCGN_04680: HEPCGN_04680 - Protein, at 815,955 to 816,557
_04680
HEPCGN_04685: yobI - Uncharacterized protein YobI, at 816,628 to 816,693
yobI
HEPCGN_04690: ruvA - Holliday junction branch migration protein RuvA, at 816,832 to 817,443
ruvA
HEPCGN_04695: ruvB - Holliday junction branch migration DNA helicase RuvB, at 817,452 to 818,462
ruvB
Position (kb) 816
817 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 815.766 kb on + strand, within ruvC at 815.862 kb on + strand, within ruvC at 815.862 kb on + strand, within ruvC at 815.867 kb on - strand, within ruvC at 815.952 kb on - strand at 815.958 kb on + strand at 816.302 kb on + strand, within HEPCGN_04680 at 816.324 kb on + strand, within HEPCGN_04680 at 816.332 kb on - strand, within HEPCGN_04680 at 816.425 kb on + strand, within HEPCGN_04680 at 816.503 kb on + strand at 816.503 kb on + strand at 816.504 kb on - strand at 816.504 kb on - strand at 816.639 kb on + strand, within yobI at 817.542 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB49 remove 815,766 + ruvC HEPCGN_04675 0.64 -0.8 815,862 + ruvC HEPCGN_04675 0.82 +0.3 815,862 + ruvC HEPCGN_04675 0.82 -0.2 815,867 - ruvC HEPCGN_04675 0.83 -0.7 815,952 - -0.6 815,958 + -0.2 816,302 + HEPCGN_04680 0.58 -1.7 816,324 + HEPCGN_04680 0.61 -0.0 816,332 - HEPCGN_04680 0.63 +0.9 816,425 + HEPCGN_04680 0.78 +0.1 816,503 + +2.2 816,503 + -0.6 816,504 - -0.2 816,504 - -0.5 816,639 + yobI HEPCGN_04685 0.17 -2.8 817,542 + +1.5
Or see this region's nucleotide sequence