Experiment: CEV1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnaA and tnaC are separated by 219 nucleotides tnaC and HEPCGN_13680 are separated by 241 nucleotides HEPCGN_13680 and yidC are separated by 105 nucleotides
HEPCGN_13670: tnaA - tryptophanase, at 1,485,075 to 1,486,490
tnaA
HEPCGN_13675: tnaC - tryptophanase leader peptide, at 1,486,710 to 1,486,784
tnaC
HEPCGN_13680: HEPCGN_13680 - tRNA modification GTPase MnmE, at 1,487,026 to 1,488,390
_13680
HEPCGN_13685: yidC - membrane protein insertase YidC, at 1,488,496 to 1,490,142
yidC
Position (kb)
1487
1488
1489 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1486.028 kb on + strand, within tnaA at 1486.028 kb on + strand, within tnaA at 1486.031 kb on - strand, within tnaA at 1486.031 kb on - strand, within tnaA at 1486.065 kb on - strand, within tnaA at 1486.091 kb on - strand, within tnaA at 1486.165 kb on + strand, within tnaA at 1486.165 kb on + strand, within tnaA at 1486.165 kb on + strand, within tnaA at 1486.166 kb on - strand, within tnaA at 1486.166 kb on - strand, within tnaA at 1486.166 kb on - strand, within tnaA at 1486.207 kb on + strand, within tnaA at 1486.208 kb on - strand, within tnaA at 1486.224 kb on - strand, within tnaA at 1486.397 kb on + strand at 1486.491 kb on + strand at 1486.512 kb on + strand at 1486.768 kb on - strand, within tnaC at 1486.769 kb on + strand, within tnaC at 1486.778 kb on + strand at 1486.779 kb on - strand at 1486.923 kb on - strand at 1487.038 kb on - strand at 1487.079 kb on + strand at 1487.102 kb on - strand at 1487.158 kb on + strand at 1487.213 kb on - strand, within HEPCGN_13680 at 1487.589 kb on + strand, within HEPCGN_13680 at 1487.589 kb on + strand, within HEPCGN_13680 at 1487.590 kb on - strand, within HEPCGN_13680 at 1487.693 kb on - strand, within HEPCGN_13680 at 1487.734 kb on + strand, within HEPCGN_13680 at 1487.734 kb on + strand, within HEPCGN_13680 at 1487.760 kb on + strand, within HEPCGN_13680 at 1488.197 kb on - strand, within HEPCGN_13680 at 1488.372 kb on + strand at 1488.372 kb on + strand at 1488.373 kb on - strand at 1488.373 kb on - strand at 1488.474 kb on - strand at 1488.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CEV1 remove 1,486,028 + tnaA HEPCGN_13670 0.67 +0.1 1,486,028 + tnaA HEPCGN_13670 0.67 +1.0 1,486,031 - tnaA HEPCGN_13670 0.68 +0.5 1,486,031 - tnaA HEPCGN_13670 0.68 +0.4 1,486,065 - tnaA HEPCGN_13670 0.70 +0.2 1,486,091 - tnaA HEPCGN_13670 0.72 +0.4 1,486,165 + tnaA HEPCGN_13670 0.77 +0.3 1,486,165 + tnaA HEPCGN_13670 0.77 -0.8 1,486,165 + tnaA HEPCGN_13670 0.77 -1.2 1,486,166 - tnaA HEPCGN_13670 0.77 -0.3 1,486,166 - tnaA HEPCGN_13670 0.77 +0.3 1,486,166 - tnaA HEPCGN_13670 0.77 -0.7 1,486,207 + tnaA HEPCGN_13670 0.80 -1.7 1,486,208 - tnaA HEPCGN_13670 0.80 +1.6 1,486,224 - tnaA HEPCGN_13670 0.81 -0.9 1,486,397 + -0.9 1,486,491 + -0.0 1,486,512 + +0.7 1,486,768 - tnaC HEPCGN_13675 0.77 +1.0 1,486,769 + tnaC HEPCGN_13675 0.79 +1.7 1,486,778 + -1.5 1,486,779 - -1.1 1,486,923 - -0.8 1,487,038 - -0.5 1,487,079 + -2.0 1,487,102 - -1.8 1,487,158 + -1.4 1,487,213 - HEPCGN_13680 0.14 +0.5 1,487,589 + HEPCGN_13680 0.41 -1.2 1,487,589 + HEPCGN_13680 0.41 +0.3 1,487,590 - HEPCGN_13680 0.41 +2.1 1,487,693 - HEPCGN_13680 0.49 +1.1 1,487,734 + HEPCGN_13680 0.52 +0.7 1,487,734 + HEPCGN_13680 0.52 -0.3 1,487,760 + HEPCGN_13680 0.54 -1.8 1,488,197 - HEPCGN_13680 0.86 +0.5 1,488,372 + +0.7 1,488,372 + +0.6 1,488,373 - -1.4 1,488,373 - -2.8 1,488,474 - +0.6 1,488,474 - +0.3
Or see this region's nucleotide sequence