Strain Fitness in Escherichia coli ECOR38 around HEPCGN_00810

Experiment: CEV1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcitT and citG are separated by 49 nucleotidescitG and citX overlap by 26 nucleotidescitX and citF are separated by 3 nucleotides HEPCGN_00800: citT - citrate/succinate antiporter CitT, at 88,719 to 90,182 citT HEPCGN_00805: citG - triphosphoribosyl-dephospho-CoA synthase CitG, at 90,232 to 91,110 citG HEPCGN_00810: citX - Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase, at 91,085 to 91,636 citX HEPCGN_00815: citF - citrate lyase subunit alpha, at 91,640 to 93,172 citF Position (kb) 91 92Strain fitness (log2 ratio) -2 -1 0 1 2at 90.105 kb on + strandat 90.160 kb on - strandat 90.162 kb on - strandat 90.162 kb on - strandat 90.201 kb on - strandat 90.255 kb on + strandat 90.304 kb on - strandat 90.367 kb on + strand, within citGat 90.494 kb on - strand, within citGat 90.636 kb on + strand, within citGat 90.774 kb on + strand, within citGat 90.808 kb on - strand, within citGat 90.954 kb on + strand, within citGat 90.975 kb on + strand, within citGat 90.976 kb on - strand, within citGat 91.049 kb on + strandat 91.049 kb on + strandat 91.139 kb on + strandat 91.297 kb on + strand, within citXat 91.298 kb on - strand, within citXat 91.641 kb on + strandat 92.006 kb on + strand, within citFat 92.069 kb on + strand, within citFat 92.117 kb on - strand, within citFat 92.255 kb on - strand, within citFat 92.384 kb on - strand, within citFat 92.413 kb on + strand, within citF

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Per-strain Table

Position Strand Gene LocusTag Fraction CEV1
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90,105 + +0.1
90,160 - -1.2
90,162 - -0.3
90,162 - -0.0
90,201 - +0.6
90,255 + -0.3
90,304 - -0.4
90,367 + citG HEPCGN_00805 0.15 -0.1
90,494 - citG HEPCGN_00805 0.30 -0.2
90,636 + citG HEPCGN_00805 0.46 -0.3
90,774 + citG HEPCGN_00805 0.62 +1.1
90,808 - citG HEPCGN_00805 0.66 -0.5
90,954 + citG HEPCGN_00805 0.82 -0.2
90,975 + citG HEPCGN_00805 0.85 +0.3
90,976 - citG HEPCGN_00805 0.85 +2.7
91,049 + -0.4
91,049 + -2.5
91,139 + -0.0
91,297 + citX HEPCGN_00810 0.38 -1.0
91,298 - citX HEPCGN_00810 0.39 -0.6
91,641 + +0.0
92,006 + citF HEPCGN_00815 0.24 -0.5
92,069 + citF HEPCGN_00815 0.28 -1.0
92,117 - citF HEPCGN_00815 0.31 -0.1
92,255 - citF HEPCGN_00815 0.40 -2.3
92,384 - citF HEPCGN_00815 0.49 +0.0
92,413 + citF HEPCGN_00815 0.50 +1.8

Or see this region's nucleotide sequence