Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alaC and ypdK are separated by 64 nucleotides ypdK and lpxP are separated by 370 nucleotides
HEPCGN_21580: alaC - alanine transaminase, at 3,065,380 to 3,066,618
alaC
HEPCGN_21585: ypdK - membrane protein YpdK, at 3,066,683 to 3,066,739
ypdK
HEPCGN_21590: lpxP - kdo(2)-lipid IV(A) palmitoleoyltransferase, at 3,067,110 to 3,068,030
lpxP
Position (kb)
3066
3067 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3065.784 kb on + strand, within alaC at 3065.788 kb on + strand, within alaC at 3065.816 kb on + strand, within alaC at 3065.979 kb on + strand, within alaC at 3066.032 kb on - strand, within alaC at 3066.115 kb on + strand, within alaC at 3066.115 kb on + strand, within alaC at 3066.183 kb on + strand, within alaC at 3066.186 kb on + strand, within alaC at 3066.187 kb on - strand, within alaC at 3066.305 kb on + strand, within alaC at 3066.364 kb on + strand, within alaC at 3066.365 kb on - strand, within alaC at 3066.365 kb on - strand, within alaC at 3066.479 kb on - strand, within alaC at 3066.597 kb on + strand at 3066.597 kb on + strand at 3066.597 kb on + strand at 3066.598 kb on - strand at 3066.684 kb on + strand at 3066.703 kb on + strand, within ypdK at 3066.725 kb on - strand, within ypdK at 3066.725 kb on - strand, within ypdK at 3066.757 kb on + strand at 3066.758 kb on - strand at 3066.772 kb on - strand at 3066.775 kb on + strand at 3066.775 kb on + strand at 3066.775 kb on + strand at 3066.800 kb on + strand at 3066.800 kb on + strand at 3066.801 kb on - strand at 3066.801 kb on - strand at 3066.840 kb on + strand at 3066.851 kb on + strand at 3066.912 kb on - strand at 3067.010 kb on - strand at 3067.097 kb on + strand at 3067.111 kb on + strand at 3067.118 kb on - strand at 3067.148 kb on + strand at 3067.167 kb on + strand at 3067.167 kb on + strand at 3067.171 kb on + strand at 3067.217 kb on + strand, within lpxP at 3067.273 kb on + strand, within lpxP at 3067.291 kb on + strand, within lpxP at 3067.305 kb on + strand, within lpxP at 3067.354 kb on + strand, within lpxP at 3067.354 kb on + strand, within lpxP at 3067.354 kb on + strand, within lpxP at 3067.355 kb on - strand, within lpxP at 3067.355 kb on - strand, within lpxP at 3067.359 kb on - strand, within lpxP at 3067.359 kb on - strand, within lpxP at 3067.423 kb on + strand, within lpxP at 3067.491 kb on - strand, within lpxP at 3067.559 kb on + strand, within lpxP at 3067.563 kb on - strand, within lpxP at 3067.563 kb on - strand, within lpxP at 3067.563 kb on - strand, within lpxP at 3067.574 kb on - strand, within lpxP at 3067.631 kb on + strand, within lpxP
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 3,065,784 + alaC HEPCGN_21580 0.33 -3.1 3,065,788 + alaC HEPCGN_21580 0.33 -1.1 3,065,816 + alaC HEPCGN_21580 0.35 +0.1 3,065,979 + alaC HEPCGN_21580 0.48 -0.3 3,066,032 - alaC HEPCGN_21580 0.53 -0.9 3,066,115 + alaC HEPCGN_21580 0.59 +0.0 3,066,115 + alaC HEPCGN_21580 0.59 -0.4 3,066,183 + alaC HEPCGN_21580 0.65 -0.1 3,066,186 + alaC HEPCGN_21580 0.65 -0.2 3,066,187 - alaC HEPCGN_21580 0.65 +0.4 3,066,305 + alaC HEPCGN_21580 0.75 +0.7 3,066,364 + alaC HEPCGN_21580 0.79 +1.2 3,066,365 - alaC HEPCGN_21580 0.79 -1.3 3,066,365 - alaC HEPCGN_21580 0.79 +1.2 3,066,479 - alaC HEPCGN_21580 0.89 -0.4 3,066,597 + +0.5 3,066,597 + -0.3 3,066,597 + +0.2 3,066,598 - -1.1 3,066,684 + -0.9 3,066,703 + ypdK HEPCGN_21585 0.35 -1.5 3,066,725 - ypdK HEPCGN_21585 0.74 -0.7 3,066,725 - ypdK HEPCGN_21585 0.74 -0.3 3,066,757 + -3.1 3,066,758 - -0.0 3,066,772 - -0.5 3,066,775 + +2.2 3,066,775 + +0.5 3,066,775 + +1.3 3,066,800 + -1.2 3,066,800 + -1.0 3,066,801 - +2.0 3,066,801 - +0.2 3,066,840 + +0.9 3,066,851 + -3.5 3,066,912 - +1.1 3,067,010 - -1.3 3,067,097 + -0.7 3,067,111 + +1.0 3,067,118 - +0.1 3,067,148 + -0.0 3,067,167 + -0.1 3,067,167 + -1.5 3,067,171 + +0.2 3,067,217 + lpxP HEPCGN_21590 0.12 +0.2 3,067,273 + lpxP HEPCGN_21590 0.18 -0.3 3,067,291 + lpxP HEPCGN_21590 0.20 +1.0 3,067,305 + lpxP HEPCGN_21590 0.21 -0.5 3,067,354 + lpxP HEPCGN_21590 0.26 -0.8 3,067,354 + lpxP HEPCGN_21590 0.26 -0.2 3,067,354 + lpxP HEPCGN_21590 0.26 +1.3 3,067,355 - lpxP HEPCGN_21590 0.27 -0.3 3,067,355 - lpxP HEPCGN_21590 0.27 +0.8 3,067,359 - lpxP HEPCGN_21590 0.27 -0.8 3,067,359 - lpxP HEPCGN_21590 0.27 +0.5 3,067,423 + lpxP HEPCGN_21590 0.34 +0.4 3,067,491 - lpxP HEPCGN_21590 0.41 +2.2 3,067,559 + lpxP HEPCGN_21590 0.49 +1.0 3,067,563 - lpxP HEPCGN_21590 0.49 -0.1 3,067,563 - lpxP HEPCGN_21590 0.49 -0.4 3,067,563 - lpxP HEPCGN_21590 0.49 +1.3 3,067,574 - lpxP HEPCGN_21590 0.50 +0.1 3,067,631 + lpxP HEPCGN_21590 0.57 +1.5
Or see this region's nucleotide sequence