Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt grcA and eamB are separated by 54 nucleotides eamB and HEPCGN_20480 are separated by 101 nucleotides HEPCGN_20480 and HEPCGN_20485 are separated by 3 nucleotides HEPCGN_20485 and tnp-IS1397 are separated by 106 nucleotides
HEPCGN_20470: grcA - autonomous glycyl radical cofactor GrcA, at 2,836,460 to 2,836,843
grcA
HEPCGN_20475: eamB - cysteine/O-acetylserine transporter, at 2,836,898 to 2,837,485
eamB
HEPCGN_20480: HEPCGN_20480 - LysR substrate-binding domain-containing protein, at 2,837,587 to 2,838,075
_20480
HEPCGN_20485: HEPCGN_20485 - LysR family transcriptional regulator, at 2,838,079 to 2,838,468
_20485
HEPCGN_20490: tnp-IS1397 - IS3 family IS1397 transposase, at 2,838,575 to 2,839,177
tnp-IS1397
Position (kb)
2837
2838
2839 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2836.683 kb on + strand, within grcA at 2836.720 kb on + strand, within grcA at 2836.720 kb on + strand, within grcA at 2836.721 kb on - strand, within grcA at 2836.759 kb on - strand, within grcA at 2836.780 kb on + strand, within grcA at 2836.781 kb on - strand, within grcA at 2836.822 kb on + strand at 2836.823 kb on - strand at 2836.849 kb on - strand at 2836.852 kb on + strand at 2836.853 kb on - strand at 2836.899 kb on + strand at 2836.902 kb on + strand at 2836.903 kb on - strand at 2836.965 kb on + strand, within eamB at 2837.111 kb on - strand, within eamB at 2837.111 kb on - strand, within eamB at 2837.213 kb on - strand, within eamB at 2837.239 kb on + strand, within eamB at 2837.239 kb on + strand, within eamB at 2837.239 kb on + strand, within eamB at 2837.239 kb on + strand, within eamB at 2837.239 kb on + strand, within eamB at 2837.240 kb on - strand, within eamB at 2837.241 kb on + strand, within eamB at 2837.241 kb on + strand, within eamB at 2837.241 kb on + strand, within eamB at 2837.242 kb on - strand, within eamB at 2837.242 kb on - strand, within eamB at 2837.318 kb on + strand, within eamB at 2837.334 kb on - strand, within eamB at 2837.401 kb on + strand, within eamB at 2837.413 kb on + strand, within eamB at 2837.413 kb on + strand, within eamB at 2837.414 kb on - strand, within eamB at 2837.414 kb on - strand, within eamB at 2837.414 kb on - strand, within eamB at 2837.452 kb on - strand at 2837.549 kb on + strand at 2837.605 kb on - strand at 2837.605 kb on - strand at 2837.727 kb on - strand, within HEPCGN_20480 at 2837.791 kb on + strand, within HEPCGN_20480 at 2837.792 kb on - strand, within HEPCGN_20480 at 2837.792 kb on - strand, within HEPCGN_20480 at 2837.972 kb on - strand, within HEPCGN_20480 at 2838.073 kb on + strand at 2838.074 kb on - strand at 2838.117 kb on + strand at 2838.136 kb on - strand, within HEPCGN_20485 at 2838.145 kb on - strand, within HEPCGN_20485 at 2838.154 kb on - strand, within HEPCGN_20485 at 2838.241 kb on - strand, within HEPCGN_20485 at 2838.241 kb on - strand, within HEPCGN_20485 at 2838.242 kb on + strand, within HEPCGN_20485 at 2838.242 kb on + strand, within HEPCGN_20485 at 2838.242 kb on + strand, within HEPCGN_20485 at 2838.243 kb on - strand, within HEPCGN_20485 at 2838.246 kb on + strand, within HEPCGN_20485 at 2838.280 kb on - strand, within HEPCGN_20485 at 2838.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 2,836,683 + grcA HEPCGN_20470 0.58 +0.6 2,836,720 + grcA HEPCGN_20470 0.68 -0.1 2,836,720 + grcA HEPCGN_20470 0.68 +0.3 2,836,721 - grcA HEPCGN_20470 0.68 -2.1 2,836,759 - grcA HEPCGN_20470 0.78 -0.3 2,836,780 + grcA HEPCGN_20470 0.83 +4.5 2,836,781 - grcA HEPCGN_20470 0.84 +0.0 2,836,822 + +1.4 2,836,823 - -0.6 2,836,849 - +0.6 2,836,852 + +0.3 2,836,853 - -0.0 2,836,899 + +0.4 2,836,902 + -0.9 2,836,903 - -3.1 2,836,965 + eamB HEPCGN_20475 0.11 -0.3 2,837,111 - eamB HEPCGN_20475 0.36 -0.8 2,837,111 - eamB HEPCGN_20475 0.36 +0.4 2,837,213 - eamB HEPCGN_20475 0.54 -0.5 2,837,239 + eamB HEPCGN_20475 0.58 +0.4 2,837,239 + eamB HEPCGN_20475 0.58 +0.4 2,837,239 + eamB HEPCGN_20475 0.58 +2.0 2,837,239 + eamB HEPCGN_20475 0.58 +1.0 2,837,239 + eamB HEPCGN_20475 0.58 -0.2 2,837,240 - eamB HEPCGN_20475 0.58 -0.4 2,837,241 + eamB HEPCGN_20475 0.58 +0.8 2,837,241 + eamB HEPCGN_20475 0.58 +0.7 2,837,241 + eamB HEPCGN_20475 0.58 -1.8 2,837,242 - eamB HEPCGN_20475 0.59 +0.9 2,837,242 - eamB HEPCGN_20475 0.59 -0.1 2,837,318 + eamB HEPCGN_20475 0.71 -0.4 2,837,334 - eamB HEPCGN_20475 0.74 -0.8 2,837,401 + eamB HEPCGN_20475 0.86 -0.3 2,837,413 + eamB HEPCGN_20475 0.88 -0.1 2,837,413 + eamB HEPCGN_20475 0.88 -1.0 2,837,414 - eamB HEPCGN_20475 0.88 -0.1 2,837,414 - eamB HEPCGN_20475 0.88 -0.7 2,837,414 - eamB HEPCGN_20475 0.88 -0.1 2,837,452 - -1.7 2,837,549 + +0.3 2,837,605 - +0.4 2,837,605 - -0.4 2,837,727 - HEPCGN_20480 0.29 -1.0 2,837,791 + HEPCGN_20480 0.42 -3.1 2,837,792 - HEPCGN_20480 0.42 -0.6 2,837,792 - HEPCGN_20480 0.42 -1.8 2,837,972 - HEPCGN_20480 0.79 -0.3 2,838,073 + +0.2 2,838,074 - +0.7 2,838,117 + -0.3 2,838,136 - HEPCGN_20485 0.15 -1.8 2,838,145 - HEPCGN_20485 0.17 +0.0 2,838,154 - HEPCGN_20485 0.19 +0.0 2,838,241 - HEPCGN_20485 0.42 -0.6 2,838,241 - HEPCGN_20485 0.42 -2.2 2,838,242 + HEPCGN_20485 0.42 +0.4 2,838,242 + HEPCGN_20485 0.42 -1.6 2,838,242 + HEPCGN_20485 0.42 +0.3 2,838,243 - HEPCGN_20485 0.42 +0.1 2,838,246 + HEPCGN_20485 0.43 +0.5 2,838,280 - HEPCGN_20485 0.52 +0.2 2,838,473 - +1.4
Or see this region's nucleotide sequence