Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiA and HEPCGN_16040 are separated by 104 nucleotides HEPCGN_16040 and fic overlap by 11 nucleotides fic and pabA are separated by 31 nucleotides pabA and argD,dapC are separated by 84 nucleotides
HEPCGN_16035: ppiA - peptidylprolyl isomerase A, at 1,981,485 to 1,982,057
ppiA
HEPCGN_16040: HEPCGN_16040 - DUF2559 domain-containing protein, at 1,982,162 to 1,982,329
_16040
HEPCGN_16045: fic - putative adenosine monophosphate-protein transferase Fic, at 1,982,319 to 1,982,921
fic
HEPCGN_16050: pabA - aminodeoxychorismate synthase component 2, at 1,982,953 to 1,983,516
pabA
HEPCGN_16055: argD,dapC - bifunctional acetylornithine/succinyldiaminopimelate transaminase, at 1,983,601 to 1,984,824
argD,dapC
Position (kb)
1982
1983 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1981.375 kb on + strand at 1981.376 kb on - strand at 1981.376 kb on - strand at 1981.376 kb on - strand at 1981.381 kb on + strand at 1981.381 kb on + strand at 1981.391 kb on - strand at 1981.395 kb on - strand at 1981.400 kb on + strand at 1981.405 kb on - strand at 1981.431 kb on + strand at 1981.432 kb on - strand at 1981.436 kb on - strand at 1981.436 kb on - strand at 1981.576 kb on + strand, within ppiA at 1981.577 kb on - strand, within ppiA at 1981.577 kb on - strand, within ppiA at 1981.677 kb on + strand, within ppiA at 1981.678 kb on - strand, within ppiA at 1981.679 kb on + strand, within ppiA at 1981.679 kb on + strand, within ppiA at 1981.791 kb on - strand, within ppiA at 1981.927 kb on + strand, within ppiA at 1982.030 kb on + strand at 1982.030 kb on + strand at 1982.030 kb on + strand at 1982.031 kb on - strand at 1982.055 kb on + strand at 1982.125 kb on + strand at 1982.125 kb on + strand at 1982.126 kb on - strand at 1982.212 kb on + strand, within HEPCGN_16040 at 1982.219 kb on - strand, within HEPCGN_16040 at 1982.262 kb on + strand, within HEPCGN_16040 at 1982.318 kb on + strand at 1982.319 kb on - strand at 1982.358 kb on + strand at 1982.373 kb on - strand at 1982.518 kb on - strand, within fic at 1982.561 kb on + strand, within fic at 1982.561 kb on + strand, within fic at 1982.598 kb on + strand, within fic at 1982.599 kb on - strand, within fic at 1982.650 kb on - strand, within fic at 1982.697 kb on + strand, within fic at 1982.700 kb on + strand, within fic at 1982.881 kb on + strand at 1982.881 kb on + strand at 1982.881 kb on + strand at 1982.886 kb on - strand at 1982.886 kb on - strand at 1982.896 kb on + strand at 1982.974 kb on + strand at 1982.975 kb on - strand at 1982.975 kb on - strand at 1983.158 kb on - strand, within pabA at 1983.279 kb on + strand, within pabA at 1983.293 kb on + strand, within pabA at 1983.294 kb on - strand, within pabA at 1983.465 kb on + strand at 1983.531 kb on + strand at 1983.584 kb on - strand at 1983.584 kb on - strand at 1983.584 kb on - strand at 1983.662 kb on - strand at 1983.687 kb on - strand at 1983.730 kb on + strand, within argD,dapC at 1983.730 kb on + strand, within argD,dapC at 1983.731 kb on - strand, within argD,dapC at 1983.828 kb on + strand, within argD,dapC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 1,981,375 + -1.0 1,981,376 - +1.4 1,981,376 - -0.5 1,981,376 - +0.6 1,981,381 + -0.4 1,981,381 + -1.0 1,981,391 - +2.9 1,981,395 - +0.2 1,981,400 + -0.0 1,981,405 - -0.1 1,981,431 + +0.3 1,981,432 - +0.1 1,981,436 - -0.7 1,981,436 - +1.1 1,981,576 + ppiA HEPCGN_16035 0.16 +1.2 1,981,577 - ppiA HEPCGN_16035 0.16 +0.5 1,981,577 - ppiA HEPCGN_16035 0.16 -1.0 1,981,677 + ppiA HEPCGN_16035 0.34 +0.4 1,981,678 - ppiA HEPCGN_16035 0.34 -0.1 1,981,679 + ppiA HEPCGN_16035 0.34 +0.4 1,981,679 + ppiA HEPCGN_16035 0.34 -0.1 1,981,791 - ppiA HEPCGN_16035 0.53 -0.4 1,981,927 + ppiA HEPCGN_16035 0.77 +0.3 1,982,030 + +0.6 1,982,030 + -0.5 1,982,030 + -0.3 1,982,031 - -1.1 1,982,055 + -0.2 1,982,125 + +0.1 1,982,125 + +0.5 1,982,126 - -0.5 1,982,212 + HEPCGN_16040 0.30 +1.9 1,982,219 - HEPCGN_16040 0.34 -0.0 1,982,262 + HEPCGN_16040 0.60 -0.0 1,982,318 + +0.0 1,982,319 - -0.1 1,982,358 + +0.6 1,982,373 - +2.8 1,982,518 - fic HEPCGN_16045 0.33 -0.7 1,982,561 + fic HEPCGN_16045 0.40 -1.4 1,982,561 + fic HEPCGN_16045 0.40 -1.8 1,982,598 + fic HEPCGN_16045 0.46 -1.6 1,982,599 - fic HEPCGN_16045 0.46 +0.4 1,982,650 - fic HEPCGN_16045 0.55 -1.8 1,982,697 + fic HEPCGN_16045 0.63 -0.2 1,982,700 + fic HEPCGN_16045 0.63 -0.7 1,982,881 + -0.6 1,982,881 + +0.2 1,982,881 + +0.4 1,982,886 - -1.5 1,982,886 - +0.8 1,982,896 + +0.5 1,982,974 + +1.6 1,982,975 - +0.3 1,982,975 - +0.3 1,983,158 - pabA HEPCGN_16050 0.36 +0.3 1,983,279 + pabA HEPCGN_16050 0.58 -0.1 1,983,293 + pabA HEPCGN_16050 0.60 +1.0 1,983,294 - pabA HEPCGN_16050 0.60 +1.6 1,983,465 + -0.1 1,983,531 + -0.1 1,983,584 - +0.5 1,983,584 - +1.5 1,983,584 - -0.3 1,983,662 - -0.6 1,983,687 - -0.2 1,983,730 + argD,dapC HEPCGN_16055 0.11 -1.0 1,983,730 + argD,dapC HEPCGN_16055 0.11 -1.4 1,983,731 - argD,dapC HEPCGN_16055 0.11 -0.4 1,983,828 + argD,dapC HEPCGN_16055 0.19 -0.4
Or see this region's nucleotide sequence