Strain Fitness in Escherichia coli ECOR38 around HEPCGN_12930

Experiment: Bas18

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiB and ubiJ overlap by 4 nucleotidesubiJ and ubiE are separated by 13 nucleotidesubiE and rmuC are separated by 94 nucleotides HEPCGN_12920: ubiB - ubiquinone biosynthesis regulatory protein kinase UbiB, at 1,333,382 to 1,335,022 ubiB HEPCGN_12930: ubiJ - ubiquinone biosynthesis protein UbiJ, at 1,335,019 to 1,335,624 ubiJ HEPCGN_12935: ubiE - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 1,335,638 to 1,336,393 ubiE HEPCGN_12940: rmuC - DNA recombination protein RmuC, at 1,336,488 to 1,337,915 rmuC Position (kb) 1335 1336Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1335.449 kb on - strand, within ubiJat 1335.449 kb on - strand, within ubiJat 1335.503 kb on - strand, within ubiJat 1335.616 kb on - strandat 1335.647 kb on - strandat 1335.647 kb on - strandat 1335.647 kb on - strandat 1335.647 kb on - strandat 1335.774 kb on + strand, within ubiEat 1335.781 kb on - strand, within ubiEat 1335.838 kb on - strand, within ubiEat 1336.109 kb on - strand, within ubiEat 1336.471 kb on + strandat 1336.472 kb on - strandat 1336.505 kb on + strandat 1336.505 kb on + strandat 1336.506 kb on - strandat 1336.525 kb on + strandat 1336.567 kb on + strandat 1336.610 kb on + strandat 1336.623 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas18
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1,335,449 - ubiJ HEPCGN_12930 0.71 -0.5
1,335,449 - ubiJ HEPCGN_12930 0.71 -3.3
1,335,503 - ubiJ HEPCGN_12930 0.80 -0.9
1,335,616 - -1.0
1,335,647 - -0.6
1,335,647 - +3.0
1,335,647 - -0.3
1,335,647 - -0.9
1,335,774 + ubiE HEPCGN_12935 0.18 -0.8
1,335,781 - ubiE HEPCGN_12935 0.19 -0.8
1,335,838 - ubiE HEPCGN_12935 0.26 -1.1
1,336,109 - ubiE HEPCGN_12935 0.62 -0.3
1,336,471 + -0.1
1,336,472 - -0.5
1,336,505 + +0.3
1,336,505 + -0.2
1,336,506 - -0.8
1,336,525 + +1.0
1,336,567 + +0.7
1,336,610 + -0.4
1,336,623 - -2.3

Or see this region's nucleotide sequence