Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hupA and yjaG are separated by 186 nucleotides yjaG and nfi are separated by 42 nucleotides nfi and hemE are separated by 9 nucleotides
HEPCGN_11980: hupA - DNA-binding protein HU-alpha, at 1,137,081 to 1,137,353
hupA
HEPCGN_11985: yjaG - Uncharacterized protein YjaG, at 1,137,540 to 1,138,130
yjaG
HEPCGN_11990: nfi - deoxyribonuclease V, at 1,138,173 to 1,138,844
nfi
HEPCGN_11995: hemE - uroporphyrinogen decarboxylase, at 1,138,854 to 1,139,918
hemE
Position (kb) 1138
1139 Strain fitness (log2 ratio) -1
0
1
2
3 at 1137.229 kb on - strand, within hupA at 1137.257 kb on + strand, within hupA at 1137.327 kb on + strand at 1137.328 kb on - strand at 1137.328 kb on - strand at 1137.353 kb on + strand at 1137.364 kb on + strand at 1137.365 kb on - strand at 1137.385 kb on + strand at 1137.386 kb on - strand at 1137.386 kb on - strand at 1137.415 kb on + strand at 1137.430 kb on + strand at 1137.430 kb on + strand at 1137.431 kb on - strand at 1137.504 kb on + strand at 1137.504 kb on + strand at 1137.522 kb on - strand at 1137.542 kb on - strand at 1137.640 kb on - strand, within yjaG at 1137.651 kb on + strand, within yjaG at 1137.681 kb on + strand, within yjaG at 1137.832 kb on - strand, within yjaG at 1137.933 kb on + strand, within yjaG at 1138.033 kb on + strand, within yjaG at 1138.034 kb on - strand, within yjaG at 1138.037 kb on + strand, within yjaG at 1138.037 kb on + strand, within yjaG at 1138.043 kb on - strand, within yjaG at 1138.043 kb on - strand, within yjaG at 1138.174 kb on + strand at 1138.190 kb on + strand at 1138.191 kb on - strand at 1138.338 kb on - strand, within nfi at 1138.462 kb on - strand, within nfi at 1138.483 kb on - strand, within nfi at 1138.549 kb on - strand, within nfi at 1138.549 kb on - strand, within nfi at 1138.549 kb on - strand, within nfi at 1138.627 kb on + strand, within nfi at 1138.627 kb on + strand, within nfi at 1138.628 kb on - strand, within nfi at 1138.628 kb on - strand, within nfi at 1138.628 kb on - strand, within nfi at 1138.659 kb on - strand, within nfi at 1138.660 kb on + strand, within nfi at 1138.828 kb on - strand at 1138.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 1,137,229 - hupA HEPCGN_11980 0.54 +2.0 1,137,257 + hupA HEPCGN_11980 0.64 +1.6 1,137,327 + +1.9 1,137,328 - +2.1 1,137,328 - +1.8 1,137,353 + +2.4 1,137,364 + +3.1 1,137,365 - +1.7 1,137,385 + +0.9 1,137,386 - +0.3 1,137,386 - +0.9 1,137,415 + +3.2 1,137,430 + +2.6 1,137,430 + +0.7 1,137,431 - -0.3 1,137,504 + -0.7 1,137,504 + +0.6 1,137,522 - +0.9 1,137,542 - +0.1 1,137,640 - yjaG HEPCGN_11985 0.17 +0.4 1,137,651 + yjaG HEPCGN_11985 0.19 -0.6 1,137,681 + yjaG HEPCGN_11985 0.24 +0.6 1,137,832 - yjaG HEPCGN_11985 0.49 +0.1 1,137,933 + yjaG HEPCGN_11985 0.66 -0.2 1,138,033 + yjaG HEPCGN_11985 0.83 +1.5 1,138,034 - yjaG HEPCGN_11985 0.84 +0.5 1,138,037 + yjaG HEPCGN_11985 0.84 +3.0 1,138,037 + yjaG HEPCGN_11985 0.84 -1.3 1,138,043 - yjaG HEPCGN_11985 0.85 +0.0 1,138,043 - yjaG HEPCGN_11985 0.85 +0.8 1,138,174 + +0.0 1,138,190 + +0.0 1,138,191 - -0.1 1,138,338 - nfi HEPCGN_11990 0.25 +0.4 1,138,462 - nfi HEPCGN_11990 0.43 -0.1 1,138,483 - nfi HEPCGN_11990 0.46 -1.1 1,138,549 - nfi HEPCGN_11990 0.56 +0.2 1,138,549 - nfi HEPCGN_11990 0.56 +1.2 1,138,549 - nfi HEPCGN_11990 0.56 -0.8 1,138,627 + nfi HEPCGN_11990 0.68 -0.5 1,138,627 + nfi HEPCGN_11990 0.68 +1.0 1,138,628 - nfi HEPCGN_11990 0.68 -0.7 1,138,628 - nfi HEPCGN_11990 0.68 -0.0 1,138,628 - nfi HEPCGN_11990 0.68 -0.3 1,138,659 - nfi HEPCGN_11990 0.72 -0.5 1,138,660 + nfi HEPCGN_11990 0.72 -0.3 1,138,828 - +0.2 1,138,856 - +0.4
Or see this region's nucleotide sequence