Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ulaA and sgaB are separated by 22 nucleotides sgaB and HEPCGN_10940 are separated by 45 nucleotides HEPCGN_10940 and HEPCGN_10945 are separated by 257 nucleotides
HEPCGN_10930: ulaA - PTS ascorbate transporter subunit IIC, at 928,903 to 930,291
ulaA
HEPCGN_10935: sgaB - PTS mannitol transporter subunit IIB, at 930,314 to 930,619
sgaB
HEPCGN_10940: HEPCGN_10940 - PTS ascorbate transporter subunit IIA, at 930,665 to 931,108
_10940
HEPCGN_10945: HEPCGN_10945 - LacI family transcriptional regulator, at 931,366 to 932,385
_10945
Position (kb) 930
931 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 929.332 kb on + strand, within ulaA at 929.332 kb on + strand, within ulaA at 929.333 kb on - strand, within ulaA at 929.333 kb on - strand, within ulaA at 929.431 kb on + strand, within ulaA at 929.431 kb on + strand, within ulaA at 929.432 kb on - strand, within ulaA at 929.466 kb on + strand, within ulaA at 929.467 kb on - strand, within ulaA at 929.473 kb on + strand, within ulaA at 929.474 kb on - strand, within ulaA at 929.500 kb on + strand, within ulaA at 929.519 kb on + strand, within ulaA at 929.520 kb on - strand, within ulaA at 929.520 kb on - strand, within ulaA at 929.520 kb on - strand, within ulaA at 929.520 kb on - strand, within ulaA at 929.586 kb on - strand, within ulaA at 929.766 kb on + strand, within ulaA at 929.767 kb on - strand, within ulaA at 929.767 kb on - strand, within ulaA at 929.775 kb on + strand, within ulaA at 929.848 kb on + strand, within ulaA at 930.250 kb on - strand at 930.285 kb on + strand at 930.361 kb on + strand, within sgaB at 930.656 kb on - strand at 930.812 kb on - strand, within HEPCGN_10940 at 930.867 kb on - strand, within HEPCGN_10940 at 930.876 kb on - strand, within HEPCGN_10940 at 930.942 kb on + strand, within HEPCGN_10940 at 930.943 kb on - strand, within HEPCGN_10940 at 930.945 kb on + strand, within HEPCGN_10940 at 930.946 kb on - strand, within HEPCGN_10940 at 930.988 kb on - strand, within HEPCGN_10940 at 931.001 kb on + strand, within HEPCGN_10940 at 931.033 kb on + strand, within HEPCGN_10940 at 931.033 kb on + strand, within HEPCGN_10940 at 931.034 kb on - strand, within HEPCGN_10940 at 931.037 kb on + strand, within HEPCGN_10940 at 931.071 kb on - strand at 931.102 kb on + strand at 931.155 kb on - strand at 931.165 kb on + strand at 931.165 kb on + strand at 931.166 kb on - strand at 931.206 kb on - strand at 931.206 kb on - strand at 931.273 kb on - strand at 931.275 kb on + strand at 931.282 kb on + strand at 931.284 kb on + strand at 931.320 kb on + strand at 931.320 kb on + strand at 931.321 kb on - strand at 931.321 kb on - strand at 931.321 kb on - strand at 931.324 kb on + strand at 931.325 kb on - strand at 931.327 kb on + strand at 931.327 kb on + strand at 931.340 kb on + strand at 931.340 kb on + strand at 931.340 kb on + strand at 931.345 kb on - strand at 931.533 kb on + strand, within HEPCGN_10945
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 929,332 + ulaA HEPCGN_10930 0.31 -0.8 929,332 + ulaA HEPCGN_10930 0.31 +0.1 929,333 - ulaA HEPCGN_10930 0.31 +0.1 929,333 - ulaA HEPCGN_10930 0.31 +0.3 929,431 + ulaA HEPCGN_10930 0.38 -0.2 929,431 + ulaA HEPCGN_10930 0.38 -0.5 929,432 - ulaA HEPCGN_10930 0.38 +0.4 929,466 + ulaA HEPCGN_10930 0.41 +0.2 929,467 - ulaA HEPCGN_10930 0.41 +0.5 929,473 + ulaA HEPCGN_10930 0.41 -2.9 929,474 - ulaA HEPCGN_10930 0.41 -0.2 929,500 + ulaA HEPCGN_10930 0.43 +0.1 929,519 + ulaA HEPCGN_10930 0.44 +2.2 929,520 - ulaA HEPCGN_10930 0.44 -0.2 929,520 - ulaA HEPCGN_10930 0.44 -0.5 929,520 - ulaA HEPCGN_10930 0.44 -0.1 929,520 - ulaA HEPCGN_10930 0.44 +0.6 929,586 - ulaA HEPCGN_10930 0.49 +0.5 929,766 + ulaA HEPCGN_10930 0.62 +0.4 929,767 - ulaA HEPCGN_10930 0.62 +0.2 929,767 - ulaA HEPCGN_10930 0.62 -0.5 929,775 + ulaA HEPCGN_10930 0.63 +0.5 929,848 + ulaA HEPCGN_10930 0.68 +0.6 930,250 - -0.8 930,285 + -0.5 930,361 + sgaB HEPCGN_10935 0.15 -0.7 930,656 - -0.5 930,812 - HEPCGN_10940 0.33 -0.6 930,867 - HEPCGN_10940 0.45 +0.1 930,876 - HEPCGN_10940 0.48 +0.1 930,942 + HEPCGN_10940 0.62 +0.2 930,943 - HEPCGN_10940 0.63 +0.7 930,945 + HEPCGN_10940 0.63 -0.9 930,946 - HEPCGN_10940 0.63 -0.9 930,988 - HEPCGN_10940 0.73 -0.3 931,001 + HEPCGN_10940 0.76 +0.4 931,033 + HEPCGN_10940 0.83 -0.6 931,033 + HEPCGN_10940 0.83 -1.8 931,034 - HEPCGN_10940 0.83 +0.3 931,037 + HEPCGN_10940 0.84 -0.1 931,071 - -0.4 931,102 + +0.1 931,155 - +0.5 931,165 + -1.4 931,165 + -0.8 931,166 - +0.1 931,206 - +0.4 931,206 - -0.0 931,273 - -0.3 931,275 + +0.3 931,282 + +0.4 931,284 + +0.1 931,320 + +0.4 931,320 + -0.1 931,321 - -2.4 931,321 - -2.4 931,321 - -1.8 931,324 + +0.7 931,325 - +0.5 931,327 + +0.9 931,327 + -0.4 931,340 + +0.9 931,340 + -1.2 931,340 + -0.2 931,345 - +1.3 931,533 + HEPCGN_10945 0.16 -1.4
Or see this region's nucleotide sequence