Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ulaF and ulaE overlap by 1 nucleotides ulaE and ulaD are separated by 9 nucleotides ulaD and ulaC are separated by 13 nucleotides ulaC and ulaB are separated by 9 nucleotides ulaB and ulaA are separated by 15 nucleotides
HEPCGN_10355: ulaF - L-ribulose-5-phosphate 4-epimerase UlaF, at 815,674 to 816,360
ulaF
HEPCGN_10360: ulaE - L-ribulose-5-phosphate 3-epimerase UlaE, at 816,360 to 817,214
ulaE
HEPCGN_10365: ulaD - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 817,224 to 817,874
ulaD
HEPCGN_10370: ulaC - PTS ascorbate transporter subunit IIA, at 817,888 to 818,352
ulaC
HEPCGN_10375: ulaB - PTS ascorbate transporter subunit IIB, at 818,362 to 818,667
ulaB
HEPCGN_10380: ulaA - PTS ascorbate transporter subunit IIC, at 818,683 to 820,080
ulaA
Position (kb) 817
818 Strain fitness (log2 ratio) -2
-1
0
1 at 816.292 kb on - strand at 816.338 kb on - strand at 816.361 kb on + strand at 816.528 kb on + strand, within ulaE at 816.597 kb on - strand, within ulaE at 816.694 kb on + strand, within ulaE at 816.876 kb on + strand, within ulaE at 816.914 kb on + strand, within ulaE at 817.096 kb on - strand, within ulaE at 817.096 kb on - strand, within ulaE at 817.183 kb on + strand at 817.183 kb on + strand at 817.184 kb on - strand at 817.214 kb on + strand at 817.214 kb on + strand at 817.299 kb on - strand, within ulaD at 817.781 kb on + strand, within ulaD at 817.827 kb on - strand at 817.889 kb on + strand at 817.890 kb on - strand at 817.952 kb on - strand, within ulaC at 817.952 kb on - strand, within ulaC at 818.041 kb on - strand, within ulaC at 818.183 kb on + strand, within ulaC at 818.183 kb on + strand, within ulaC at 818.184 kb on - strand, within ulaC at 818.225 kb on + strand, within ulaC at 818.225 kb on + strand, within ulaC at 818.226 kb on - strand, within ulaC at 818.363 kb on + strand at 818.363 kb on + strand at 818.364 kb on - strand at 818.456 kb on + strand, within ulaB at 818.456 kb on + strand, within ulaB at 818.456 kb on + strand, within ulaB at 818.456 kb on + strand, within ulaB at 818.457 kb on - strand, within ulaB at 818.496 kb on + strand, within ulaB at 818.497 kb on - strand, within ulaB at 818.656 kb on - strand at 818.660 kb on - strand at 818.682 kb on - strand at 818.754 kb on + strand at 818.754 kb on + strand at 818.755 kb on - strand at 818.756 kb on + strand at 818.757 kb on - strand at 818.757 kb on - strand at 818.770 kb on + strand at 818.770 kb on + strand at 818.788 kb on - strand at 818.788 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 816,292 - -0.6 816,338 - -0.2 816,361 + +0.7 816,528 + ulaE HEPCGN_10360 0.20 -1.6 816,597 - ulaE HEPCGN_10360 0.28 +0.3 816,694 + ulaE HEPCGN_10360 0.39 +0.7 816,876 + ulaE HEPCGN_10360 0.60 +1.0 816,914 + ulaE HEPCGN_10360 0.65 -1.2 817,096 - ulaE HEPCGN_10360 0.86 +1.1 817,096 - ulaE HEPCGN_10360 0.86 +0.5 817,183 + -2.0 817,183 + +0.2 817,184 - +0.6 817,214 + +1.2 817,214 + -0.6 817,299 - ulaD HEPCGN_10365 0.12 -0.4 817,781 + ulaD HEPCGN_10365 0.86 -1.8 817,827 - -0.1 817,889 + -0.5 817,890 - -0.8 817,952 - ulaC HEPCGN_10370 0.14 -0.2 817,952 - ulaC HEPCGN_10370 0.14 +0.8 818,041 - ulaC HEPCGN_10370 0.33 +0.9 818,183 + ulaC HEPCGN_10370 0.63 +0.2 818,183 + ulaC HEPCGN_10370 0.63 -0.4 818,184 - ulaC HEPCGN_10370 0.64 +0.2 818,225 + ulaC HEPCGN_10370 0.72 +0.4 818,225 + ulaC HEPCGN_10370 0.72 +0.3 818,226 - ulaC HEPCGN_10370 0.73 -0.0 818,363 + -0.2 818,363 + +0.4 818,364 - -0.1 818,456 + ulaB HEPCGN_10375 0.31 +0.3 818,456 + ulaB HEPCGN_10375 0.31 -2.0 818,456 + ulaB HEPCGN_10375 0.31 -0.4 818,456 + ulaB HEPCGN_10375 0.31 +1.2 818,457 - ulaB HEPCGN_10375 0.31 -0.6 818,496 + ulaB HEPCGN_10375 0.44 +0.1 818,497 - ulaB HEPCGN_10375 0.44 +0.4 818,656 - -0.1 818,660 - +0.2 818,682 - +1.2 818,754 + +0.2 818,754 + +0.1 818,755 - -0.3 818,756 + +1.5 818,757 - -0.3 818,757 - +0.4 818,770 + -0.8 818,770 + +0.1 818,788 - -1.2 818,788 - -0.1
Or see this region's nucleotide sequence