Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gapA and msrB are separated by 341 nucleotides msrB and yeaC are separated by 43 nucleotides yeaC and ydjL are separated by 369 nucleotides
HEPCGN_05120: gapA - glyceraldehyde-3-phosphate dehydrogenase, at 899,257 to 900,252
gapA
HEPCGN_05125: msrB - Peptide methionine sulfoxide reductase MsrB, at 900,594 to 901,007
msrB
HEPCGN_05130: yeaC - Uncharacterized protein YeaC, at 901,051 to 901,323
yeaC
HEPCGN_05135: ydjL - Uncharacterized zinc-type alcohol dehydrogenase-like protein YdjL, at 901,693 to 902,769
ydjL
Position (kb) 901
902 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 900.365 kb on + strand at 900.365 kb on + strand at 900.366 kb on - strand at 900.546 kb on + strand at 900.560 kb on + strand at 900.560 kb on + strand at 900.561 kb on - strand at 900.561 kb on - strand at 900.575 kb on + strand at 900.652 kb on - strand, within msrB at 900.652 kb on - strand, within msrB at 900.698 kb on - strand, within msrB at 900.704 kb on + strand, within msrB at 900.705 kb on - strand, within msrB at 900.721 kb on + strand, within msrB at 900.723 kb on + strand, within msrB at 900.723 kb on + strand, within msrB at 900.771 kb on + strand, within msrB at 900.802 kb on - strand, within msrB at 900.831 kb on + strand, within msrB at 900.831 kb on + strand, within msrB at 900.833 kb on + strand, within msrB at 900.833 kb on + strand, within msrB at 900.866 kb on - strand, within msrB at 900.964 kb on + strand, within msrB at 900.964 kb on + strand, within msrB at 901.206 kb on + strand, within yeaC at 901.207 kb on - strand, within yeaC at 901.305 kb on + strand at 901.317 kb on + strand at 901.321 kb on + strand at 901.353 kb on - strand at 901.353 kb on - strand at 901.353 kb on - strand at 901.371 kb on + strand at 901.483 kb on + strand at 901.497 kb on + strand at 901.497 kb on + strand at 901.498 kb on - strand at 901.498 kb on - strand at 901.567 kb on + strand at 901.568 kb on - strand at 901.651 kb on - strand at 901.664 kb on - strand at 901.664 kb on - strand at 901.828 kb on + strand, within ydjL at 901.858 kb on - strand, within ydjL at 901.891 kb on - strand, within ydjL at 902.043 kb on + strand, within ydjL at 902.068 kb on + strand, within ydjL at 902.141 kb on - strand, within ydjL at 902.156 kb on + strand, within ydjL at 902.291 kb on + strand, within ydjL at 902.291 kb on + strand, within ydjL at 902.295 kb on + strand, within ydjL at 902.313 kb on - strand, within ydjL at 902.313 kb on - strand, within ydjL
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 900,365 + -0.5 900,365 + -0.6 900,366 - +0.4 900,546 + -0.3 900,560 + -0.1 900,560 + +0.4 900,561 - -0.8 900,561 - -0.0 900,575 + +0.1 900,652 - msrB HEPCGN_05125 0.14 -1.1 900,652 - msrB HEPCGN_05125 0.14 +0.2 900,698 - msrB HEPCGN_05125 0.25 -0.5 900,704 + msrB HEPCGN_05125 0.27 +1.0 900,705 - msrB HEPCGN_05125 0.27 -0.1 900,721 + msrB HEPCGN_05125 0.31 -0.4 900,723 + msrB HEPCGN_05125 0.31 -0.2 900,723 + msrB HEPCGN_05125 0.31 -0.5 900,771 + msrB HEPCGN_05125 0.43 +0.4 900,802 - msrB HEPCGN_05125 0.50 +0.6 900,831 + msrB HEPCGN_05125 0.57 -0.8 900,831 + msrB HEPCGN_05125 0.57 +0.1 900,833 + msrB HEPCGN_05125 0.58 +0.4 900,833 + msrB HEPCGN_05125 0.58 +0.0 900,866 - msrB HEPCGN_05125 0.66 -0.2 900,964 + msrB HEPCGN_05125 0.89 +0.2 900,964 + msrB HEPCGN_05125 0.89 -1.0 901,206 + yeaC HEPCGN_05130 0.57 -0.9 901,207 - yeaC HEPCGN_05130 0.57 -0.8 901,305 + +0.1 901,317 + -0.5 901,321 + -0.8 901,353 - -0.9 901,353 - -0.6 901,353 - -0.2 901,371 + +0.6 901,483 + +0.2 901,497 + -0.5 901,497 + -0.7 901,498 - -2.2 901,498 - -1.4 901,567 + -0.2 901,568 - +3.0 901,651 - -0.3 901,664 - +0.8 901,664 - -0.1 901,828 + ydjL HEPCGN_05135 0.13 -1.3 901,858 - ydjL HEPCGN_05135 0.15 +0.7 901,891 - ydjL HEPCGN_05135 0.18 +1.8 902,043 + ydjL HEPCGN_05135 0.32 +0.4 902,068 + ydjL HEPCGN_05135 0.35 -0.2 902,141 - ydjL HEPCGN_05135 0.42 -0.1 902,156 + ydjL HEPCGN_05135 0.43 -0.1 902,291 + ydjL HEPCGN_05135 0.56 +0.6 902,291 + ydjL HEPCGN_05135 0.56 +0.9 902,295 + ydjL HEPCGN_05135 0.56 -0.2 902,313 - ydjL HEPCGN_05135 0.58 -0.5 902,313 - ydjL HEPCGN_05135 0.58 -0.2
Or see this region's nucleotide sequence