Experiment: Bas18
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolA and tolB are separated by 132 nucleotides tolB and pal are separated by 34 nucleotides pal and cpoB are separated by 9 nucleotides
HEPCGN_01460: tolA - cell envelope integrity protein TolA, at 221,218 to 222,405
tolA
HEPCGN_01465: tolB - Tol-Pal system protein TolB, at 222,538 to 223,830
tolB
HEPCGN_01470: pal - peptidoglycan-associated lipoprotein Pal, at 223,865 to 224,386
pal
HEPCGN_01475: cpoB - cell division protein CpoB, at 224,396 to 225,187
cpoB
Position (kb) 222
223
224 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 222.153 kb on + strand, within tolA at 222.153 kb on + strand, within tolA at 222.154 kb on - strand, within tolA at 222.154 kb on - strand, within tolA at 222.157 kb on + strand, within tolA at 222.172 kb on - strand, within tolA at 222.190 kb on - strand, within tolA at 222.197 kb on - strand, within tolA at 222.211 kb on + strand, within tolA at 222.240 kb on - strand, within tolA at 222.258 kb on + strand, within tolA at 222.258 kb on + strand, within tolA at 222.258 kb on + strand, within tolA at 222.259 kb on - strand, within tolA at 222.259 kb on - strand, within tolA at 222.259 kb on - strand, within tolA at 222.324 kb on + strand at 222.364 kb on + strand at 222.403 kb on + strand at 222.465 kb on - strand at 222.534 kb on + strand at 222.535 kb on - strand at 222.552 kb on - strand at 222.575 kb on + strand at 222.645 kb on + strand at 222.795 kb on - strand, within tolB at 222.795 kb on - strand, within tolB at 222.822 kb on + strand, within tolB at 222.822 kb on + strand, within tolB at 222.833 kb on + strand, within tolB at 222.833 kb on + strand, within tolB at 222.871 kb on + strand, within tolB at 222.871 kb on + strand, within tolB at 222.920 kb on + strand, within tolB at 223.096 kb on + strand, within tolB at 223.096 kb on + strand, within tolB at 223.183 kb on + strand, within tolB at 223.184 kb on - strand, within tolB at 223.402 kb on - strand, within tolB at 223.449 kb on + strand, within tolB at 223.449 kb on + strand, within tolB at 223.450 kb on - strand, within tolB at 223.482 kb on + strand, within tolB at 223.483 kb on - strand, within tolB at 223.515 kb on + strand, within tolB at 223.515 kb on + strand, within tolB at 223.516 kb on - strand, within tolB at 223.518 kb on + strand, within tolB at 223.572 kb on + strand, within tolB at 223.578 kb on - strand, within tolB at 223.640 kb on + strand, within tolB at 223.708 kb on + strand at 223.708 kb on + strand at 223.709 kb on - strand at 223.709 kb on - strand at 223.752 kb on + strand at 223.832 kb on - strand at 223.835 kb on - strand at 224.079 kb on - strand, within pal at 224.079 kb on - strand, within pal at 224.096 kb on + strand, within pal at 224.203 kb on + strand, within pal at 224.306 kb on + strand, within pal at 224.329 kb on - strand, within pal at 224.352 kb on - strand at 224.382 kb on - strand at 224.405 kb on - strand at 224.421 kb on + strand at 224.446 kb on + strand at 224.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas18 remove 222,153 + tolA HEPCGN_01460 0.79 -0.1 222,153 + tolA HEPCGN_01460 0.79 -1.5 222,154 - tolA HEPCGN_01460 0.79 +0.3 222,154 - tolA HEPCGN_01460 0.79 +0.4 222,157 + tolA HEPCGN_01460 0.79 +1.7 222,172 - tolA HEPCGN_01460 0.80 -0.1 222,190 - tolA HEPCGN_01460 0.82 +0.3 222,197 - tolA HEPCGN_01460 0.82 -0.6 222,211 + tolA HEPCGN_01460 0.84 -0.8 222,240 - tolA HEPCGN_01460 0.86 -0.6 222,258 + tolA HEPCGN_01460 0.88 +3.9 222,258 + tolA HEPCGN_01460 0.88 -2.3 222,258 + tolA HEPCGN_01460 0.88 -2.2 222,259 - tolA HEPCGN_01460 0.88 -1.2 222,259 - tolA HEPCGN_01460 0.88 -2.2 222,259 - tolA HEPCGN_01460 0.88 -2.5 222,324 + -2.0 222,364 + -1.8 222,403 + +0.2 222,465 - +0.4 222,534 + -0.7 222,535 - -0.0 222,552 - -0.7 222,575 + +1.3 222,645 + -2.0 222,795 - tolB HEPCGN_01465 0.20 -2.5 222,795 - tolB HEPCGN_01465 0.20 -0.2 222,822 + tolB HEPCGN_01465 0.22 -2.2 222,822 + tolB HEPCGN_01465 0.22 +1.1 222,833 + tolB HEPCGN_01465 0.23 -0.1 222,833 + tolB HEPCGN_01465 0.23 -2.0 222,871 + tolB HEPCGN_01465 0.26 -1.9 222,871 + tolB HEPCGN_01465 0.26 -1.9 222,920 + tolB HEPCGN_01465 0.30 -0.2 223,096 + tolB HEPCGN_01465 0.43 -2.6 223,096 + tolB HEPCGN_01465 0.43 +0.3 223,183 + tolB HEPCGN_01465 0.50 -2.6 223,184 - tolB HEPCGN_01465 0.50 -1.3 223,402 - tolB HEPCGN_01465 0.67 -1.5 223,449 + tolB HEPCGN_01465 0.70 -0.4 223,449 + tolB HEPCGN_01465 0.70 -2.9 223,450 - tolB HEPCGN_01465 0.71 -0.0 223,482 + tolB HEPCGN_01465 0.73 +0.1 223,483 - tolB HEPCGN_01465 0.73 +0.4 223,515 + tolB HEPCGN_01465 0.76 -0.3 223,515 + tolB HEPCGN_01465 0.76 -1.3 223,516 - tolB HEPCGN_01465 0.76 +0.9 223,518 + tolB HEPCGN_01465 0.76 +0.4 223,572 + tolB HEPCGN_01465 0.80 -1.9 223,578 - tolB HEPCGN_01465 0.80 -1.3 223,640 + tolB HEPCGN_01465 0.85 -3.5 223,708 + -0.9 223,708 + -1.3 223,709 - +0.9 223,709 - -0.2 223,752 + -1.0 223,832 - -1.3 223,835 - -2.1 224,079 - pal HEPCGN_01470 0.41 -1.0 224,079 - pal HEPCGN_01470 0.41 +0.9 224,096 + pal HEPCGN_01470 0.44 -2.6 224,203 + pal HEPCGN_01470 0.65 -1.6 224,306 + pal HEPCGN_01470 0.84 -3.0 224,329 - pal HEPCGN_01470 0.89 +5.5 224,352 - -2.6 224,382 - +0.3 224,405 - -0.1 224,421 + -0.6 224,446 + +0.9 224,449 - -0.3
Or see this region's nucleotide sequence