Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysB and ymiA are separated by 330 nucleotides ymiA and HEPCGN_25610 are separated by 2 nucleotides HEPCGN_25610 and ymiC are separated by 128 nucleotides ymiC and acnA are separated by 164 nucleotides
HEPCGN_25600: cysB - HTH-type transcriptional regulator CysB, at 3,857,870 to 3,858,844
cysB
HEPCGN_25605: ymiA - YmiA family putative membrane protein, at 3,859,175 to 3,859,303
ymiA
HEPCGN_25610: HEPCGN_25610 - Protein, C-ter, truncated protein, at 3,859,306 to 3,859,473
_25610
HEPCGN_25620: ymiC - Protein YmiC, at 3,859,602 to 3,859,682
ymiC
HEPCGN_25625: acnA - aconitate hydratase AcnA, at 3,859,847 to 3,862,522
acnA
Position (kb)
3859
3860 Strain fitness (log2 ratio)
-1
0
1 at 3858.459 kb on - strand, within cysB at 3858.460 kb on + strand, within cysB at 3858.460 kb on + strand, within cysB at 3858.461 kb on - strand, within cysB at 3858.673 kb on + strand, within cysB at 3858.673 kb on + strand, within cysB at 3858.701 kb on - strand, within cysB at 3858.871 kb on + strand at 3858.981 kb on - strand at 3859.018 kb on - strand at 3859.041 kb on - strand at 3859.163 kb on - strand at 3859.163 kb on - strand at 3859.215 kb on + strand, within ymiA at 3859.215 kb on + strand, within ymiA at 3859.305 kb on + strand at 3859.305 kb on + strand at 3859.305 kb on + strand at 3859.306 kb on - strand at 3859.306 kb on - strand at 3859.340 kb on + strand, within HEPCGN_25610 at 3859.340 kb on + strand, within HEPCGN_25610 at 3859.581 kb on + strand at 3859.587 kb on - strand at 3859.601 kb on + strand at 3859.674 kb on - strand, within ymiC at 3859.791 kb on + strand at 3859.861 kb on - strand at 3859.861 kb on - strand at 3859.913 kb on + strand at 3860.315 kb on + strand, within acnA at 3860.315 kb on + strand, within acnA at 3860.315 kb on + strand, within acnA
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 3,858,459 - cysB HEPCGN_25600 0.60 -1.3 3,858,460 + cysB HEPCGN_25600 0.61 -0.6 3,858,460 + cysB HEPCGN_25600 0.61 +0.3 3,858,461 - cysB HEPCGN_25600 0.61 +0.6 3,858,673 + cysB HEPCGN_25600 0.82 -0.1 3,858,673 + cysB HEPCGN_25600 0.82 +0.3 3,858,701 - cysB HEPCGN_25600 0.85 -0.6 3,858,871 + -1.1 3,858,981 - +0.2 3,859,018 - -1.2 3,859,041 - +0.9 3,859,163 - +0.1 3,859,163 - +0.4 3,859,215 + ymiA HEPCGN_25605 0.31 +0.0 3,859,215 + ymiA HEPCGN_25605 0.31 -0.3 3,859,305 + -0.2 3,859,305 + -0.2 3,859,305 + -1.5 3,859,306 - -1.0 3,859,306 - +0.1 3,859,340 + HEPCGN_25610 0.20 +0.1 3,859,340 + HEPCGN_25610 0.20 -1.4 3,859,581 + +0.8 3,859,587 - -0.3 3,859,601 + -0.4 3,859,674 - ymiC HEPCGN_25620 0.89 +0.6 3,859,791 + +0.1 3,859,861 - +0.3 3,859,861 - -0.3 3,859,913 + +0.2 3,860,315 + acnA HEPCGN_25625 0.17 -0.4 3,860,315 + acnA HEPCGN_25625 0.17 -0.2 3,860,315 + acnA HEPCGN_25625 0.17 -0.3
Or see this region's nucleotide sequence