Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truD and surE overlap by 20 nucleotides surE and pcm overlap by 7 nucleotides pcm and nlpD are separated by 139 nucleotides
HEPCGN_19655: truD - tRNA pseudouridine(13) synthase TruD, at 2,687,545 to 2,688,594
truD
HEPCGN_19660: surE - 5'/3'-nucleotidase SurE, at 2,688,575 to 2,689,336
surE
HEPCGN_19665: pcm - protein-L-isoaspartate O-methyltransferase, at 2,689,330 to 2,689,956
pcm
HEPCGN_19670: nlpD - murein hydrolase activator NlpD, at 2,690,096 to 2,691,235
nlpD
Position (kb)
2689
2690 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2688.399 kb on - strand, within truD at 2688.399 kb on - strand, within truD at 2688.467 kb on + strand, within truD at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.556 kb on - strand at 2688.556 kb on - strand at 2688.556 kb on - strand at 2688.556 kb on - strand at 2688.575 kb on - strand at 2688.607 kb on - strand at 2688.607 kb on - strand at 2688.622 kb on - strand at 2688.622 kb on - strand at 2688.736 kb on + strand, within surE at 2688.737 kb on - strand, within surE at 2688.754 kb on + strand, within surE at 2688.878 kb on - strand, within surE at 2688.958 kb on + strand, within surE at 2688.959 kb on - strand, within surE at 2688.965 kb on + strand, within surE at 2689.043 kb on - strand, within surE at 2689.043 kb on - strand, within surE at 2689.084 kb on + strand, within surE at 2689.084 kb on + strand, within surE at 2689.085 kb on - strand, within surE at 2689.105 kb on + strand, within surE at 2689.106 kb on - strand, within surE at 2689.106 kb on - strand, within surE at 2689.156 kb on + strand, within surE at 2689.156 kb on + strand, within surE at 2689.224 kb on + strand, within surE at 2689.225 kb on - strand, within surE at 2689.235 kb on + strand, within surE at 2689.236 kb on - strand, within surE at 2689.236 kb on - strand, within surE at 2689.236 kb on - strand, within surE at 2689.266 kb on + strand at 2689.286 kb on + strand at 2689.286 kb on + strand at 2689.287 kb on - strand at 2689.287 kb on - strand at 2689.287 kb on - strand at 2689.287 kb on - strand at 2689.346 kb on + strand at 2689.346 kb on + strand at 2689.380 kb on + strand at 2689.381 kb on - strand at 2689.484 kb on + strand, within pcm at 2689.514 kb on - strand, within pcm at 2689.515 kb on + strand, within pcm at 2689.515 kb on + strand, within pcm at 2689.698 kb on + strand, within pcm at 2689.861 kb on + strand, within pcm at 2689.969 kb on + strand at 2689.970 kb on - strand at 2689.988 kb on + strand at 2690.037 kb on - strand at 2690.058 kb on - strand at 2690.058 kb on - strand at 2690.058 kb on - strand at 2690.093 kb on + strand at 2690.094 kb on - strand at 2690.285 kb on - strand, within nlpD at 2690.386 kb on - strand, within nlpD at 2690.458 kb on + strand, within nlpD at 2690.462 kb on + strand, within nlpD at 2690.462 kb on + strand, within nlpD at 2690.464 kb on + strand, within nlpD at 2690.464 kb on + strand, within nlpD at 2690.464 kb on + strand, within nlpD at 2690.465 kb on - strand, within nlpD at 2690.494 kb on + strand, within nlpD at 2690.495 kb on - strand, within nlpD at 2690.506 kb on + strand, within nlpD at 2690.545 kb on + strand, within nlpD at 2690.558 kb on + strand, within nlpD at 2690.584 kb on - strand, within nlpD at 2690.716 kb on + strand, within nlpD at 2690.716 kb on + strand, within nlpD at 2690.716 kb on + strand, within nlpD at 2690.717 kb on - strand, within nlpD at 2690.721 kb on - strand, within nlpD at 2690.803 kb on - strand, within nlpD at 2690.845 kb on + strand, within nlpD at 2690.845 kb on + strand, within nlpD at 2690.845 kb on + strand, within nlpD at 2690.846 kb on - strand, within nlpD at 2690.846 kb on - strand, within nlpD at 2690.864 kb on - strand, within nlpD
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 2,688,399 - truD HEPCGN_19655 0.81 +0.4 2,688,399 - truD HEPCGN_19655 0.81 +2.1 2,688,467 + truD HEPCGN_19655 0.88 +0.3 2,688,555 + -0.1 2,688,555 + +0.3 2,688,555 + -1.2 2,688,555 + -0.2 2,688,555 + +0.6 2,688,556 - -1.7 2,688,556 - -0.9 2,688,556 - -0.0 2,688,556 - +0.5 2,688,575 - +0.3 2,688,607 - -1.1 2,688,607 - -0.6 2,688,622 - +0.1 2,688,622 - -0.7 2,688,736 + surE HEPCGN_19660 0.21 +0.6 2,688,737 - surE HEPCGN_19660 0.21 +1.9 2,688,754 + surE HEPCGN_19660 0.23 +0.7 2,688,878 - surE HEPCGN_19660 0.40 +0.4 2,688,958 + surE HEPCGN_19660 0.50 +0.1 2,688,959 - surE HEPCGN_19660 0.50 -0.1 2,688,965 + surE HEPCGN_19660 0.51 +0.5 2,689,043 - surE HEPCGN_19660 0.61 +0.2 2,689,043 - surE HEPCGN_19660 0.61 -0.5 2,689,084 + surE HEPCGN_19660 0.67 +0.2 2,689,084 + surE HEPCGN_19660 0.67 +0.8 2,689,085 - surE HEPCGN_19660 0.67 +0.6 2,689,105 + surE HEPCGN_19660 0.70 +0.3 2,689,106 - surE HEPCGN_19660 0.70 +1.0 2,689,106 - surE HEPCGN_19660 0.70 -0.4 2,689,156 + surE HEPCGN_19660 0.76 -0.3 2,689,156 + surE HEPCGN_19660 0.76 -0.2 2,689,224 + surE HEPCGN_19660 0.85 -1.4 2,689,225 - surE HEPCGN_19660 0.85 +0.6 2,689,235 + surE HEPCGN_19660 0.87 -1.9 2,689,236 - surE HEPCGN_19660 0.87 +0.3 2,689,236 - surE HEPCGN_19660 0.87 -0.7 2,689,236 - surE HEPCGN_19660 0.87 +1.6 2,689,266 + +0.9 2,689,286 + +0.3 2,689,286 + +1.1 2,689,287 - +1.4 2,689,287 - -1.2 2,689,287 - -0.2 2,689,287 - +0.8 2,689,346 + +0.4 2,689,346 + +0.2 2,689,380 + -0.2 2,689,381 - -0.6 2,689,484 + pcm HEPCGN_19665 0.25 -0.0 2,689,514 - pcm HEPCGN_19665 0.29 -0.7 2,689,515 + pcm HEPCGN_19665 0.30 -1.4 2,689,515 + pcm HEPCGN_19665 0.30 -1.3 2,689,698 + pcm HEPCGN_19665 0.59 +0.5 2,689,861 + pcm HEPCGN_19665 0.85 +0.6 2,689,969 + -0.7 2,689,970 - -0.1 2,689,988 + -1.6 2,690,037 - -0.6 2,690,058 - -1.2 2,690,058 - +0.5 2,690,058 - +2.1 2,690,093 + -0.6 2,690,094 - -0.6 2,690,285 - nlpD HEPCGN_19670 0.17 +1.7 2,690,386 - nlpD HEPCGN_19670 0.25 +1.2 2,690,458 + nlpD HEPCGN_19670 0.32 -0.6 2,690,462 + nlpD HEPCGN_19670 0.32 +0.5 2,690,462 + nlpD HEPCGN_19670 0.32 -0.3 2,690,464 + nlpD HEPCGN_19670 0.32 -0.4 2,690,464 + nlpD HEPCGN_19670 0.32 -0.0 2,690,464 + nlpD HEPCGN_19670 0.32 +0.6 2,690,465 - nlpD HEPCGN_19670 0.32 +0.1 2,690,494 + nlpD HEPCGN_19670 0.35 +0.7 2,690,495 - nlpD HEPCGN_19670 0.35 +1.2 2,690,506 + nlpD HEPCGN_19670 0.36 +0.2 2,690,545 + nlpD HEPCGN_19670 0.39 +1.6 2,690,558 + nlpD HEPCGN_19670 0.41 -0.2 2,690,584 - nlpD HEPCGN_19670 0.43 -0.2 2,690,716 + nlpD HEPCGN_19670 0.54 +1.4 2,690,716 + nlpD HEPCGN_19670 0.54 +1.4 2,690,716 + nlpD HEPCGN_19670 0.54 +0.7 2,690,717 - nlpD HEPCGN_19670 0.54 +2.1 2,690,721 - nlpD HEPCGN_19670 0.55 +1.0 2,690,803 - nlpD HEPCGN_19670 0.62 +1.6 2,690,845 + nlpD HEPCGN_19670 0.66 +1.1 2,690,845 + nlpD HEPCGN_19670 0.66 +0.8 2,690,845 + nlpD HEPCGN_19670 0.66 +3.0 2,690,846 - nlpD HEPCGN_19670 0.66 +1.8 2,690,846 - nlpD HEPCGN_19670 0.66 +1.6 2,690,864 - nlpD HEPCGN_19670 0.67 +1.7
Or see this region's nucleotide sequence