Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hsrA and rbsR overlap by 35 nucleotides rbsR and rbsK are separated by 3 nucleotides rbsK and rbsB are separated by 125 nucleotides
HEPCGN_13430: hsrA - putative transport protein HsrA, at 1,430,041 to 1,431,468
hsrA
HEPCGN_13435: rbsR - ribose operon transcriptional repressor RbsR, at 1,431,434 to 1,432,426
rbsR
HEPCGN_13440: rbsK - ribokinase, at 1,432,430 to 1,433,359
rbsK
HEPCGN_13445: rbsB - ribose ABC transporter substrate-binding protein RbsB, at 1,433,485 to 1,434,369
rbsB
Position (kb)
1432
1433
1434 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1431.466 kb on + strand at 1431.518 kb on + strand at 1431.518 kb on + strand at 1431.518 kb on + strand at 1431.518 kb on + strand at 1431.519 kb on - strand at 1431.519 kb on - strand at 1431.519 kb on - strand at 1431.519 kb on - strand at 1431.563 kb on - strand, within rbsR at 1431.569 kb on + strand, within rbsR at 1431.729 kb on - strand, within rbsR at 1431.743 kb on - strand, within rbsR at 1431.880 kb on + strand, within rbsR at 1431.880 kb on + strand, within rbsR at 1431.880 kb on + strand, within rbsR at 1431.880 kb on + strand, within rbsR at 1431.881 kb on - strand, within rbsR at 1431.915 kb on + strand, within rbsR at 1431.926 kb on + strand, within rbsR at 1431.927 kb on - strand, within rbsR at 1431.950 kb on - strand, within rbsR at 1431.950 kb on - strand, within rbsR at 1431.959 kb on - strand, within rbsR at 1432.003 kb on + strand, within rbsR at 1432.156 kb on + strand, within rbsR at 1432.156 kb on + strand, within rbsR at 1432.156 kb on + strand, within rbsR at 1432.156 kb on + strand, within rbsR at 1432.157 kb on - strand, within rbsR at 1432.157 kb on - strand, within rbsR at 1432.157 kb on - strand, within rbsR at 1432.220 kb on - strand, within rbsR at 1432.246 kb on + strand, within rbsR at 1432.294 kb on - strand, within rbsR at 1432.307 kb on - strand, within rbsR at 1432.357 kb on + strand at 1432.358 kb on - strand at 1432.385 kb on - strand at 1432.385 kb on - strand at 1432.420 kb on + strand at 1432.421 kb on - strand at 1432.496 kb on - strand at 1432.594 kb on + strand, within rbsK at 1432.673 kb on + strand, within rbsK at 1432.673 kb on + strand, within rbsK at 1432.674 kb on - strand, within rbsK at 1432.688 kb on + strand, within rbsK at 1432.693 kb on + strand, within rbsK at 1432.694 kb on - strand, within rbsK at 1432.706 kb on - strand, within rbsK at 1432.771 kb on + strand, within rbsK at 1432.808 kb on - strand, within rbsK at 1432.811 kb on - strand, within rbsK at 1432.876 kb on + strand, within rbsK at 1432.877 kb on - strand, within rbsK at 1432.943 kb on + strand, within rbsK at 1432.944 kb on - strand, within rbsK at 1432.946 kb on + strand, within rbsK at 1432.947 kb on - strand, within rbsK at 1432.966 kb on + strand, within rbsK at 1432.966 kb on + strand, within rbsK at 1433.027 kb on - strand, within rbsK at 1433.027 kb on - strand, within rbsK at 1433.053 kb on + strand, within rbsK at 1433.110 kb on + strand, within rbsK at 1433.110 kb on + strand, within rbsK at 1433.155 kb on + strand, within rbsK at 1433.171 kb on - strand, within rbsK at 1433.171 kb on - strand, within rbsK at 1433.200 kb on + strand, within rbsK at 1433.253 kb on + strand, within rbsK at 1433.254 kb on - strand, within rbsK at 1433.302 kb on + strand at 1433.303 kb on - strand at 1433.303 kb on - strand at 1433.320 kb on + strand at 1433.321 kb on - strand at 1433.420 kb on - strand at 1433.424 kb on + strand at 1433.425 kb on - strand at 1433.447 kb on + strand at 1433.451 kb on + strand at 1433.452 kb on - strand at 1433.469 kb on + strand at 1433.469 kb on + strand at 1433.469 kb on + strand at 1433.470 kb on - strand at 1433.481 kb on - strand at 1433.494 kb on - strand at 1433.524 kb on - strand at 1433.548 kb on + strand at 1433.653 kb on - strand, within rbsB at 1433.744 kb on - strand, within rbsB at 1433.838 kb on + strand, within rbsB at 1433.898 kb on + strand, within rbsB at 1433.957 kb on + strand, within rbsB at 1433.957 kb on + strand, within rbsB at 1433.958 kb on - strand, within rbsB at 1434.142 kb on + strand, within rbsB at 1434.206 kb on + strand, within rbsB at 1434.207 kb on - strand, within rbsB at 1434.268 kb on - strand, within rbsB at 1434.268 kb on - strand, within rbsB
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 1,431,466 + +1.1 1,431,518 + +0.9 1,431,518 + +0.6 1,431,518 + +0.5 1,431,518 + +1.2 1,431,519 - +1.2 1,431,519 - -0.7 1,431,519 - +1.2 1,431,519 - +1.2 1,431,563 - rbsR HEPCGN_13435 0.13 +1.4 1,431,569 + rbsR HEPCGN_13435 0.14 +1.5 1,431,729 - rbsR HEPCGN_13435 0.30 +1.5 1,431,743 - rbsR HEPCGN_13435 0.31 +0.9 1,431,880 + rbsR HEPCGN_13435 0.45 +3.3 1,431,880 + rbsR HEPCGN_13435 0.45 +1.1 1,431,880 + rbsR HEPCGN_13435 0.45 +0.9 1,431,880 + rbsR HEPCGN_13435 0.45 -0.0 1,431,881 - rbsR HEPCGN_13435 0.45 +1.0 1,431,915 + rbsR HEPCGN_13435 0.48 +1.1 1,431,926 + rbsR HEPCGN_13435 0.50 -0.4 1,431,927 - rbsR HEPCGN_13435 0.50 +1.4 1,431,950 - rbsR HEPCGN_13435 0.52 +0.6 1,431,950 - rbsR HEPCGN_13435 0.52 +0.7 1,431,959 - rbsR HEPCGN_13435 0.53 +1.6 1,432,003 + rbsR HEPCGN_13435 0.57 +1.0 1,432,156 + rbsR HEPCGN_13435 0.73 +0.7 1,432,156 + rbsR HEPCGN_13435 0.73 +1.1 1,432,156 + rbsR HEPCGN_13435 0.73 +0.9 1,432,156 + rbsR HEPCGN_13435 0.73 +0.0 1,432,157 - rbsR HEPCGN_13435 0.73 +1.6 1,432,157 - rbsR HEPCGN_13435 0.73 +1.0 1,432,157 - rbsR HEPCGN_13435 0.73 +0.1 1,432,220 - rbsR HEPCGN_13435 0.79 +1.2 1,432,246 + rbsR HEPCGN_13435 0.82 +1.0 1,432,294 - rbsR HEPCGN_13435 0.87 +3.7 1,432,307 - rbsR HEPCGN_13435 0.88 +1.4 1,432,357 + +1.4 1,432,358 - +1.5 1,432,385 - +0.3 1,432,385 - +1.2 1,432,420 + +1.0 1,432,421 - +1.2 1,432,496 - +1.3 1,432,594 + rbsK HEPCGN_13440 0.18 +0.4 1,432,673 + rbsK HEPCGN_13440 0.26 +0.7 1,432,673 + rbsK HEPCGN_13440 0.26 +1.2 1,432,674 - rbsK HEPCGN_13440 0.26 -0.1 1,432,688 + rbsK HEPCGN_13440 0.28 -0.4 1,432,693 + rbsK HEPCGN_13440 0.28 +0.9 1,432,694 - rbsK HEPCGN_13440 0.28 +1.0 1,432,706 - rbsK HEPCGN_13440 0.30 +1.2 1,432,771 + rbsK HEPCGN_13440 0.37 +0.9 1,432,808 - rbsK HEPCGN_13440 0.41 +1.6 1,432,811 - rbsK HEPCGN_13440 0.41 +0.8 1,432,876 + rbsK HEPCGN_13440 0.48 -0.3 1,432,877 - rbsK HEPCGN_13440 0.48 +0.9 1,432,943 + rbsK HEPCGN_13440 0.55 +1.1 1,432,944 - rbsK HEPCGN_13440 0.55 +0.4 1,432,946 + rbsK HEPCGN_13440 0.55 -0.1 1,432,947 - rbsK HEPCGN_13440 0.56 +0.2 1,432,966 + rbsK HEPCGN_13440 0.58 +1.5 1,432,966 + rbsK HEPCGN_13440 0.58 +0.7 1,433,027 - rbsK HEPCGN_13440 0.64 +1.0 1,433,027 - rbsK HEPCGN_13440 0.64 +1.2 1,433,053 + rbsK HEPCGN_13440 0.67 +1.0 1,433,110 + rbsK HEPCGN_13440 0.73 +0.5 1,433,110 + rbsK HEPCGN_13440 0.73 +1.8 1,433,155 + rbsK HEPCGN_13440 0.78 -0.0 1,433,171 - rbsK HEPCGN_13440 0.80 +0.2 1,433,171 - rbsK HEPCGN_13440 0.80 +0.3 1,433,200 + rbsK HEPCGN_13440 0.83 +0.9 1,433,253 + rbsK HEPCGN_13440 0.88 +1.9 1,433,254 - rbsK HEPCGN_13440 0.89 +2.2 1,433,302 + +0.8 1,433,303 - +1.9 1,433,303 - -0.8 1,433,320 + +1.8 1,433,321 - +1.1 1,433,420 - +0.1 1,433,424 + +0.2 1,433,425 - +1.0 1,433,447 + +0.4 1,433,451 + +0.2 1,433,452 - -0.1 1,433,469 + -1.7 1,433,469 + +0.8 1,433,469 + -1.5 1,433,470 - -0.5 1,433,481 - -0.6 1,433,494 - +0.3 1,433,524 - +0.2 1,433,548 + +0.5 1,433,653 - rbsB HEPCGN_13445 0.19 -0.3 1,433,744 - rbsB HEPCGN_13445 0.29 -0.1 1,433,838 + rbsB HEPCGN_13445 0.40 +2.4 1,433,898 + rbsB HEPCGN_13445 0.47 -2.3 1,433,957 + rbsB HEPCGN_13445 0.53 +1.5 1,433,957 + rbsB HEPCGN_13445 0.53 -0.0 1,433,958 - rbsB HEPCGN_13445 0.53 -1.2 1,434,142 + rbsB HEPCGN_13445 0.74 +0.0 1,434,206 + rbsB HEPCGN_13445 0.81 +0.1 1,434,207 - rbsB HEPCGN_13445 0.82 -0.7 1,434,268 - rbsB HEPCGN_13445 0.88 -1.0 1,434,268 - rbsB HEPCGN_13445 0.88 -1.4
Or see this region's nucleotide sequence