Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt betA and HEPCGN_07615 are separated by 193 nucleotides HEPCGN_07615 and lutC are separated by 242 nucleotides lutC and ykgF overlap by 8 nucleotides
HEPCGN_07610: betA - choline dehydrogenase, at 262,817 to 264,505
betA
HEPCGN_07615: HEPCGN_07615 - hypothetical protein, at 264,699 to 265,199
_07615
HEPCGN_07620: lutC - lactate utilization protein C, at 265,442 to 266,137
lutC
HEPCGN_07625: ykgF - LutB/LldF family L-lactate oxidation iron-sulfur protein, at 266,130 to 267,557
ykgF
Position (kb) 265
266
267 Strain fitness (log2 ratio) -2
-1
0
1 at 264.449 kb on + strand at 264.449 kb on + strand at 264.449 kb on + strand at 264.532 kb on - strand at 264.543 kb on - strand at 264.559 kb on + strand at 264.560 kb on - strand at 264.561 kb on + strand at 264.696 kb on - strand at 264.785 kb on - strand, within HEPCGN_07615 at 264.822 kb on - strand, within HEPCGN_07615 at 264.865 kb on + strand, within HEPCGN_07615 at 264.935 kb on + strand, within HEPCGN_07615 at 265.009 kb on - strand, within HEPCGN_07615 at 265.124 kb on - strand, within HEPCGN_07615 at 265.215 kb on + strand at 265.331 kb on + strand at 265.420 kb on - strand at 265.434 kb on + strand at 265.549 kb on + strand, within lutC at 265.550 kb on - strand, within lutC at 265.732 kb on - strand, within lutC at 265.741 kb on - strand, within lutC at 265.741 kb on - strand, within lutC at 265.756 kb on + strand, within lutC at 265.775 kb on + strand, within lutC at 265.775 kb on + strand, within lutC at 265.775 kb on + strand, within lutC at 265.775 kb on + strand, within lutC at 265.776 kb on - strand, within lutC at 265.913 kb on + strand, within lutC at 265.914 kb on - strand, within lutC at 265.966 kb on + strand, within lutC at 266.040 kb on + strand, within lutC at 266.040 kb on + strand, within lutC at 266.040 kb on + strand, within lutC at 266.040 kb on + strand, within lutC at 266.041 kb on - strand, within lutC at 266.047 kb on + strand, within lutC at 266.267 kb on + strand at 266.327 kb on + strand, within ykgF at 266.328 kb on - strand, within ykgF at 266.328 kb on - strand, within ykgF at 266.336 kb on - strand, within ykgF at 266.466 kb on + strand, within ykgF at 266.466 kb on + strand, within ykgF at 266.503 kb on + strand, within ykgF at 266.514 kb on + strand, within ykgF at 266.553 kb on - strand, within ykgF at 266.598 kb on - strand, within ykgF at 266.603 kb on + strand, within ykgF at 266.603 kb on + strand, within ykgF at 266.603 kb on + strand, within ykgF at 266.604 kb on - strand, within ykgF at 266.622 kb on - strand, within ykgF at 266.799 kb on + strand, within ykgF at 266.806 kb on - strand, within ykgF at 266.806 kb on - strand, within ykgF at 266.828 kb on + strand, within ykgF at 266.888 kb on + strand, within ykgF at 266.888 kb on + strand, within ykgF at 266.951 kb on + strand, within ykgF at 267.000 kb on + strand, within ykgF at 267.001 kb on - strand, within ykgF at 267.034 kb on + strand, within ykgF at 267.035 kb on - strand, within ykgF at 267.136 kb on + strand, within ykgF at 267.137 kb on - strand, within ykgF
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 264,449 + +0.7 264,449 + -2.7 264,449 + +0.5 264,532 - -0.4 264,543 - +0.6 264,559 + +1.7 264,560 - -2.5 264,561 + -0.3 264,696 - -1.5 264,785 - HEPCGN_07615 0.17 +0.4 264,822 - HEPCGN_07615 0.25 -0.1 264,865 + HEPCGN_07615 0.33 +0.4 264,935 + HEPCGN_07615 0.47 -0.7 265,009 - HEPCGN_07615 0.62 -0.6 265,124 - HEPCGN_07615 0.85 +0.5 265,215 + +0.6 265,331 + -0.1 265,420 - +0.4 265,434 + +0.4 265,549 + lutC HEPCGN_07620 0.15 -0.4 265,550 - lutC HEPCGN_07620 0.16 -0.6 265,732 - lutC HEPCGN_07620 0.42 -1.2 265,741 - lutC HEPCGN_07620 0.43 -0.7 265,741 - lutC HEPCGN_07620 0.43 +1.1 265,756 + lutC HEPCGN_07620 0.45 +1.3 265,775 + lutC HEPCGN_07620 0.48 -0.5 265,775 + lutC HEPCGN_07620 0.48 -0.5 265,775 + lutC HEPCGN_07620 0.48 -0.3 265,775 + lutC HEPCGN_07620 0.48 -2.2 265,776 - lutC HEPCGN_07620 0.48 +1.1 265,913 + lutC HEPCGN_07620 0.68 +1.3 265,914 - lutC HEPCGN_07620 0.68 +0.0 265,966 + lutC HEPCGN_07620 0.75 -1.1 266,040 + lutC HEPCGN_07620 0.86 -0.2 266,040 + lutC HEPCGN_07620 0.86 -0.9 266,040 + lutC HEPCGN_07620 0.86 -1.8 266,040 + lutC HEPCGN_07620 0.86 +1.1 266,041 - lutC HEPCGN_07620 0.86 -0.3 266,047 + lutC HEPCGN_07620 0.87 -0.8 266,267 + +0.2 266,327 + ykgF HEPCGN_07625 0.14 +0.3 266,328 - ykgF HEPCGN_07625 0.14 +0.8 266,328 - ykgF HEPCGN_07625 0.14 +0.4 266,336 - ykgF HEPCGN_07625 0.14 +0.3 266,466 + ykgF HEPCGN_07625 0.24 +0.8 266,466 + ykgF HEPCGN_07625 0.24 +0.8 266,503 + ykgF HEPCGN_07625 0.26 +0.2 266,514 + ykgF HEPCGN_07625 0.27 +0.3 266,553 - ykgF HEPCGN_07625 0.30 +1.3 266,598 - ykgF HEPCGN_07625 0.33 +1.1 266,603 + ykgF HEPCGN_07625 0.33 -0.9 266,603 + ykgF HEPCGN_07625 0.33 +1.1 266,603 + ykgF HEPCGN_07625 0.33 +0.7 266,604 - ykgF HEPCGN_07625 0.33 -2.2 266,622 - ykgF HEPCGN_07625 0.34 -0.3 266,799 + ykgF HEPCGN_07625 0.47 +0.8 266,806 - ykgF HEPCGN_07625 0.47 +1.2 266,806 - ykgF HEPCGN_07625 0.47 -0.0 266,828 + ykgF HEPCGN_07625 0.49 -0.0 266,888 + ykgF HEPCGN_07625 0.53 -0.2 266,888 + ykgF HEPCGN_07625 0.53 +0.4 266,951 + ykgF HEPCGN_07625 0.57 +0.3 267,000 + ykgF HEPCGN_07625 0.61 -0.8 267,001 - ykgF HEPCGN_07625 0.61 -0.3 267,034 + ykgF HEPCGN_07625 0.63 +0.6 267,035 - ykgF HEPCGN_07625 0.63 +0.2 267,136 + ykgF HEPCGN_07625 0.70 -0.7 267,137 - ykgF HEPCGN_07625 0.71 -0.9
Or see this region's nucleotide sequence