Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_03725 and Ile2_trna are separated by 210 nucleotides Ile2_trna and Lys_trna are separated by 1 nucleotides Lys_trna and Thr_trna are separated by 2 nucleotides Thr_trna and Gly_trna are separated by 3 nucleotides Gly_trna and HEPCGN_03750 are separated by 292 nucleotides HEPCGN_03750 and HEPCGN_03755 are separated by 4 nucleotides HEPCGN_03755 and HEPCGN_03760 are separated by 63 nucleotides
HEPCGN_03725: HEPCGN_03725 - antiterminator, at 660,405 to 661,094
_03725
HEPCGN_03730: Ile2_trna - tRNA-Ile2(cat), at 661,305 to 661,380
_trna
HEPCGN_03735: Lys_trna - tRNA-Lys(ttt), at 661,382 to 661,458
_trna
HEPCGN_03740: Thr_trna - tRNA-Thr(tgt), at 661,461 to 661,536
_trna
HEPCGN_03745: Gly_trna - tRNA-Gly(tcc), at 661,540 to 661,614
_trna
HEPCGN_03750: HEPCGN_03750 - holin, at 661,907 to 662,122
_03750
HEPCGN_03755: HEPCGN_03755 - DUF1327 domain-containing protein, at 662,127 to 662,477
_03755
HEPCGN_03760: HEPCGN_03760 - lysozyme, at 662,541 to 663,074
_03760
Position (kb) 661
662 Strain fitness (log2 ratio) -1
0
1
2 at 660.587 kb on + strand, within HEPCGN_03725 at 660.792 kb on - strand, within HEPCGN_03725 at 660.903 kb on + strand, within HEPCGN_03725 at 660.965 kb on + strand, within HEPCGN_03725 at 660.965 kb on + strand, within HEPCGN_03725 at 661.103 kb on + strand at 661.193 kb on + strand at 661.203 kb on - strand at 661.232 kb on + strand at 661.537 kb on + strand at 661.563 kb on - strand, within Gly_trna at 661.563 kb on - strand, within Gly_trna at 661.671 kb on - strand at 661.671 kb on - strand at 661.676 kb on - strand at 661.744 kb on + strand at 661.809 kb on + strand at 661.809 kb on + strand at 661.810 kb on - strand at 661.810 kb on - strand at 661.811 kb on + strand at 661.811 kb on + strand at 661.812 kb on - strand at 661.813 kb on + strand at 662.060 kb on + strand, within HEPCGN_03750 at 662.060 kb on + strand, within HEPCGN_03750 at 662.412 kb on + strand, within HEPCGN_03755 at 662.476 kb on - strand at 662.476 kb on - strand at 662.496 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 660,587 + HEPCGN_03725 0.26 +0.3 660,792 - HEPCGN_03725 0.56 +0.6 660,903 + HEPCGN_03725 0.72 -0.9 660,965 + HEPCGN_03725 0.81 +0.1 660,965 + HEPCGN_03725 0.81 -0.3 661,103 + -0.5 661,193 + -0.3 661,203 - -0.1 661,232 + +1.1 661,537 + +0.9 661,563 - Gly_trna HEPCGN_03745 0.31 +0.0 661,563 - Gly_trna HEPCGN_03745 0.31 -1.2 661,671 - -0.6 661,671 - -1.1 661,676 - -1.6 661,744 + -0.3 661,809 + -0.4 661,809 + -0.3 661,810 - +0.8 661,810 - +0.3 661,811 + -0.3 661,811 + +2.7 661,812 - +0.3 661,813 + -1.7 662,060 + HEPCGN_03750 0.71 -0.1 662,060 + HEPCGN_03750 0.71 +0.8 662,412 + HEPCGN_03755 0.81 -0.9 662,476 - -1.3 662,476 - -0.6 662,496 + -1.3
Or see this region's nucleotide sequence