Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides
HEPCGN_03190: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 550,857 to 551,468
hisIE
HEPCGN_03195: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 551,462 to 552,238
hisF
HEPCGN_03200: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 552,220 to 552,957
hisA
HEPCGN_03205: hisH - imidazole glycerol phosphate synthase subunit HisH, at 552,957 to 553,547
hisH
HEPCGN_03210: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 553,547 to 554,614
hisB
Position (kb) 552
553 Strain fitness (log2 ratio) -2
-1
0
1
2 at 551.248 kb on - strand, within hisIE at 551.298 kb on - strand, within hisIE at 551.360 kb on + strand, within hisIE at 551.361 kb on - strand, within hisIE at 551.361 kb on - strand, within hisIE at 551.362 kb on + strand, within hisIE at 551.388 kb on + strand, within hisIE at 551.414 kb on + strand at 551.468 kb on + strand at 551.468 kb on + strand at 551.469 kb on - strand at 551.500 kb on - strand at 551.541 kb on - strand, within hisF at 551.707 kb on + strand, within hisF at 551.803 kb on + strand, within hisF at 551.821 kb on - strand, within hisF at 551.841 kb on + strand, within hisF at 551.866 kb on + strand, within hisF at 551.866 kb on + strand, within hisF at 551.867 kb on - strand, within hisF at 551.867 kb on - strand, within hisF at 551.990 kb on - strand, within hisF at 552.053 kb on - strand, within hisF at 552.056 kb on + strand, within hisF at 552.057 kb on - strand, within hisF at 552.090 kb on + strand, within hisF at 552.140 kb on + strand, within hisF at 552.176 kb on + strand at 552.289 kb on + strand at 552.398 kb on + strand, within hisA at 552.400 kb on + strand, within hisA at 552.627 kb on + strand, within hisA at 552.628 kb on - strand, within hisA at 552.762 kb on + strand, within hisA at 552.762 kb on + strand, within hisA at 552.940 kb on + strand at 553.031 kb on - strand, within hisH at 553.031 kb on - strand, within hisH at 553.038 kb on - strand, within hisH at 553.077 kb on - strand, within hisH at 553.077 kb on - strand, within hisH at 553.129 kb on + strand, within hisH at 553.130 kb on - strand, within hisH at 553.130 kb on - strand, within hisH at 553.202 kb on + strand, within hisH at 553.202 kb on + strand, within hisH at 553.549 kb on - strand at 553.695 kb on + strand, within hisB at 553.696 kb on - strand, within hisB at 553.759 kb on + strand, within hisB at 553.771 kb on + strand, within hisB at 553.798 kb on + strand, within hisB at 553.880 kb on + strand, within hisB at 553.941 kb on + strand, within hisB at 553.942 kb on - strand, within hisB
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 551,248 - hisIE HEPCGN_03190 0.64 +0.2 551,298 - hisIE HEPCGN_03190 0.72 +2.7 551,360 + hisIE HEPCGN_03190 0.82 +0.3 551,361 - hisIE HEPCGN_03190 0.82 +0.0 551,361 - hisIE HEPCGN_03190 0.82 -0.6 551,362 + hisIE HEPCGN_03190 0.83 -0.1 551,388 + hisIE HEPCGN_03190 0.87 +0.7 551,414 + -0.7 551,468 + -0.2 551,468 + -0.4 551,469 - +1.1 551,500 - +0.7 551,541 - hisF HEPCGN_03195 0.10 -0.6 551,707 + hisF HEPCGN_03195 0.32 +0.3 551,803 + hisF HEPCGN_03195 0.44 +0.0 551,821 - hisF HEPCGN_03195 0.46 -2.2 551,841 + hisF HEPCGN_03195 0.49 +0.7 551,866 + hisF HEPCGN_03195 0.52 -0.4 551,866 + hisF HEPCGN_03195 0.52 -0.7 551,867 - hisF HEPCGN_03195 0.52 +1.2 551,867 - hisF HEPCGN_03195 0.52 +0.5 551,990 - hisF HEPCGN_03195 0.68 +0.3 552,053 - hisF HEPCGN_03195 0.76 -0.3 552,056 + hisF HEPCGN_03195 0.76 +1.4 552,057 - hisF HEPCGN_03195 0.77 +0.7 552,090 + hisF HEPCGN_03195 0.81 +2.7 552,140 + hisF HEPCGN_03195 0.87 +0.5 552,176 + +0.8 552,289 + -0.1 552,398 + hisA HEPCGN_03200 0.24 -0.3 552,400 + hisA HEPCGN_03200 0.24 -2.1 552,627 + hisA HEPCGN_03200 0.55 -2.2 552,628 - hisA HEPCGN_03200 0.55 +0.2 552,762 + hisA HEPCGN_03200 0.73 +0.3 552,762 + hisA HEPCGN_03200 0.73 -0.6 552,940 + -0.7 553,031 - hisH HEPCGN_03205 0.13 +0.1 553,031 - hisH HEPCGN_03205 0.13 +0.5 553,038 - hisH HEPCGN_03205 0.14 +0.3 553,077 - hisH HEPCGN_03205 0.20 -0.3 553,077 - hisH HEPCGN_03205 0.20 -0.9 553,129 + hisH HEPCGN_03205 0.29 +0.5 553,130 - hisH HEPCGN_03205 0.29 +1.9 553,130 - hisH HEPCGN_03205 0.29 +0.5 553,202 + hisH HEPCGN_03205 0.41 +0.6 553,202 + hisH HEPCGN_03205 0.41 +0.0 553,549 - +0.2 553,695 + hisB HEPCGN_03210 0.14 +0.1 553,696 - hisB HEPCGN_03210 0.14 -0.8 553,759 + hisB HEPCGN_03210 0.20 +0.0 553,771 + hisB HEPCGN_03210 0.21 +0.6 553,798 + hisB HEPCGN_03210 0.24 -0.4 553,880 + hisB HEPCGN_03210 0.31 -0.2 553,941 + hisB HEPCGN_03210 0.37 +0.2 553,942 - hisB HEPCGN_03210 0.37 -1.3
Or see this region's nucleotide sequence