Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yiaG and HEPCGN_01965 overlap by 11 nucleotides HEPCGN_01965 and rimO are separated by 225 nucleotides rimO and bssR are separated by 212 nucleotides bssR and yliI are separated by 110 nucleotides
HEPCGN_01960: yiaG - transcriptional regulator, at 320,367 to 320,696
yiaG
HEPCGN_01965: HEPCGN_01965 - addiction module toxin RelE, at 320,686 to 321,072
_01965
HEPCGN_01970: rimO - 30S ribosomal protein S12 methylthiotransferase RimO, at 321,298 to 322,623
rimO
HEPCGN_01975: bssR - biofilm formation regulator BssR, at 322,836 to 323,219
bssR
HEPCGN_01980: yliI - aldose sugar dehydrogenase YliI, at 323,330 to 324,445
yliI
Position (kb) 321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 320.406 kb on - strand, within yiaG at 320.414 kb on + strand, within yiaG at 320.448 kb on - strand, within yiaG at 320.489 kb on + strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.746 kb on - strand, within HEPCGN_01965 at 321.022 kb on - strand, within HEPCGN_01965 at 321.062 kb on + strand at 321.062 kb on + strand at 321.063 kb on - strand at 321.072 kb on + strand at 321.073 kb on - strand at 321.073 kb on - strand at 321.229 kb on - strand at 321.269 kb on + strand at 321.269 kb on + strand at 321.278 kb on + strand at 321.299 kb on + strand at 321.324 kb on - strand at 321.466 kb on - strand, within rimO at 321.618 kb on - strand, within rimO at 321.668 kb on - strand, within rimO at 321.668 kb on - strand, within rimO at 321.781 kb on + strand, within rimO at 321.915 kb on - strand, within rimO at 322.015 kb on - strand, within rimO at 322.038 kb on + strand, within rimO at 322.066 kb on - strand, within rimO at 322.079 kb on - strand, within rimO at 322.079 kb on - strand, within rimO at 322.099 kb on + strand, within rimO at 322.197 kb on - strand, within rimO at 322.202 kb on + strand, within rimO at 322.202 kb on + strand, within rimO at 322.203 kb on - strand, within rimO at 322.308 kb on - strand, within rimO at 322.311 kb on + strand, within rimO at 322.312 kb on - strand, within rimO at 322.393 kb on + strand, within rimO at 322.393 kb on + strand, within rimO at 322.499 kb on + strand at 322.499 kb on + strand at 322.499 kb on + strand at 322.500 kb on - strand at 322.500 kb on - strand at 322.545 kb on + strand at 322.545 kb on + strand at 322.546 kb on - strand at 322.794 kb on - strand at 322.800 kb on + strand at 322.801 kb on - strand at 322.801 kb on - strand at 322.835 kb on + strand at 322.836 kb on - strand at 322.905 kb on + strand, within bssR at 322.906 kb on - strand, within bssR at 322.906 kb on - strand, within bssR at 322.933 kb on - strand, within bssR at 322.933 kb on - strand, within bssR at 323.001 kb on - strand, within bssR at 323.086 kb on + strand, within bssR at 323.086 kb on + strand, within bssR at 323.086 kb on + strand, within bssR at 323.087 kb on - strand, within bssR at 323.123 kb on + strand, within bssR at 323.124 kb on - strand, within bssR at 323.127 kb on - strand, within bssR at 323.362 kb on + strand at 323.479 kb on - strand, within yliI at 323.479 kb on - strand, within yliI
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 320,406 - yiaG HEPCGN_01960 0.12 -0.7 320,414 + yiaG HEPCGN_01960 0.14 -2.2 320,448 - yiaG HEPCGN_01960 0.25 +0.5 320,489 + yiaG HEPCGN_01960 0.37 -1.9 320,490 - yiaG HEPCGN_01960 0.37 +0.4 320,490 - yiaG HEPCGN_01960 0.37 -0.2 320,490 - yiaG HEPCGN_01960 0.37 -0.3 320,490 - yiaG HEPCGN_01960 0.37 -0.6 320,746 - HEPCGN_01965 0.16 -0.8 321,022 - HEPCGN_01965 0.87 -0.8 321,062 + -0.3 321,062 + -0.3 321,063 - -1.5 321,072 + -0.9 321,073 - +0.1 321,073 - +0.4 321,229 - +1.8 321,269 + -2.3 321,269 + +1.0 321,278 + +0.7 321,299 + +0.6 321,324 - -1.7 321,466 - rimO HEPCGN_01970 0.13 -0.3 321,618 - rimO HEPCGN_01970 0.24 -0.0 321,668 - rimO HEPCGN_01970 0.28 -0.0 321,668 - rimO HEPCGN_01970 0.28 +0.8 321,781 + rimO HEPCGN_01970 0.36 +1.1 321,915 - rimO HEPCGN_01970 0.47 +0.4 322,015 - rimO HEPCGN_01970 0.54 +0.3 322,038 + rimO HEPCGN_01970 0.56 +0.5 322,066 - rimO HEPCGN_01970 0.58 -0.6 322,079 - rimO HEPCGN_01970 0.59 -0.2 322,079 - rimO HEPCGN_01970 0.59 -2.4 322,099 + rimO HEPCGN_01970 0.60 +1.4 322,197 - rimO HEPCGN_01970 0.68 -0.0 322,202 + rimO HEPCGN_01970 0.68 +0.3 322,202 + rimO HEPCGN_01970 0.68 -2.6 322,203 - rimO HEPCGN_01970 0.68 +0.2 322,308 - rimO HEPCGN_01970 0.76 -0.7 322,311 + rimO HEPCGN_01970 0.76 -0.9 322,312 - rimO HEPCGN_01970 0.76 -0.2 322,393 + rimO HEPCGN_01970 0.83 +0.5 322,393 + rimO HEPCGN_01970 0.83 -1.4 322,499 + -1.7 322,499 + +1.7 322,499 + -0.3 322,500 - -1.3 322,500 - -0.5 322,545 + +0.5 322,545 + +0.2 322,546 - +0.0 322,794 - -0.1 322,800 + -2.0 322,801 - -0.1 322,801 - -0.1 322,835 + +0.0 322,836 - +1.9 322,905 + bssR HEPCGN_01975 0.18 +0.8 322,906 - bssR HEPCGN_01975 0.18 +0.6 322,906 - bssR HEPCGN_01975 0.18 -0.3 322,933 - bssR HEPCGN_01975 0.25 +0.9 322,933 - bssR HEPCGN_01975 0.25 +0.3 323,001 - bssR HEPCGN_01975 0.43 +0.6 323,086 + bssR HEPCGN_01975 0.65 +1.2 323,086 + bssR HEPCGN_01975 0.65 -0.7 323,086 + bssR HEPCGN_01975 0.65 +0.5 323,087 - bssR HEPCGN_01975 0.65 -0.1 323,123 + bssR HEPCGN_01975 0.75 +0.4 323,124 - bssR HEPCGN_01975 0.75 -0.4 323,127 - bssR HEPCGN_01975 0.76 +0.4 323,362 + -1.9 323,479 - yliI HEPCGN_01980 0.13 -0.5 323,479 - yliI HEPCGN_01980 0.13 +0.6
Or see this region's nucleotide sequence