Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dgcI and yiaG are separated by 69 nucleotides yiaG and HEPCGN_01965 overlap by 11 nucleotides HEPCGN_01965 and rimO are separated by 225 nucleotides
HEPCGN_01955: dgcI - putative diguanylate cyclase DgcI, at 318,969 to 320,297
dgcI
HEPCGN_01960: yiaG - transcriptional regulator, at 320,367 to 320,696
yiaG
HEPCGN_01965: HEPCGN_01965 - addiction module toxin RelE, at 320,686 to 321,072
_01965
HEPCGN_01970: rimO - 30S ribosomal protein S12 methylthiotransferase RimO, at 321,298 to 322,623
rimO
Position (kb) 320
321 Strain fitness (log2 ratio) -2
-1
0
1
2 at 319.393 kb on - strand, within dgcI at 319.395 kb on + strand, within dgcI at 319.460 kb on - strand, within dgcI at 319.520 kb on + strand, within dgcI at 319.542 kb on + strand, within dgcI at 319.664 kb on + strand, within dgcI at 319.743 kb on - strand, within dgcI at 319.767 kb on - strand, within dgcI at 319.833 kb on + strand, within dgcI at 319.838 kb on + strand, within dgcI at 319.842 kb on + strand, within dgcI at 319.842 kb on + strand, within dgcI at 319.956 kb on - strand, within dgcI at 320.052 kb on - strand, within dgcI at 320.052 kb on - strand, within dgcI at 320.097 kb on + strand, within dgcI at 320.100 kb on - strand, within dgcI at 320.255 kb on + strand at 320.406 kb on - strand, within yiaG at 320.414 kb on + strand, within yiaG at 320.448 kb on - strand, within yiaG at 320.489 kb on + strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.746 kb on - strand, within HEPCGN_01965 at 321.022 kb on - strand, within HEPCGN_01965 at 321.062 kb on + strand at 321.062 kb on + strand at 321.063 kb on - strand at 321.072 kb on + strand at 321.073 kb on - strand at 321.073 kb on - strand at 321.229 kb on - strand at 321.269 kb on + strand at 321.269 kb on + strand at 321.278 kb on + strand at 321.299 kb on + strand at 321.324 kb on - strand at 321.466 kb on - strand, within rimO at 321.618 kb on - strand, within rimO at 321.668 kb on - strand, within rimO at 321.668 kb on - strand, within rimO
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 319,393 - dgcI HEPCGN_01955 0.32 -0.3 319,395 + dgcI HEPCGN_01955 0.32 -0.2 319,460 - dgcI HEPCGN_01955 0.37 +0.2 319,520 + dgcI HEPCGN_01955 0.41 -1.4 319,542 + dgcI HEPCGN_01955 0.43 -1.6 319,664 + dgcI HEPCGN_01955 0.52 -0.5 319,743 - dgcI HEPCGN_01955 0.58 -0.6 319,767 - dgcI HEPCGN_01955 0.60 -0.5 319,833 + dgcI HEPCGN_01955 0.65 +0.3 319,838 + dgcI HEPCGN_01955 0.65 -0.3 319,842 + dgcI HEPCGN_01955 0.66 +0.4 319,842 + dgcI HEPCGN_01955 0.66 -0.9 319,956 - dgcI HEPCGN_01955 0.74 +2.1 320,052 - dgcI HEPCGN_01955 0.81 -1.1 320,052 - dgcI HEPCGN_01955 0.81 +0.6 320,097 + dgcI HEPCGN_01955 0.85 +0.9 320,100 - dgcI HEPCGN_01955 0.85 +0.3 320,255 + -1.8 320,406 - yiaG HEPCGN_01960 0.12 -0.7 320,414 + yiaG HEPCGN_01960 0.14 -2.2 320,448 - yiaG HEPCGN_01960 0.25 +0.5 320,489 + yiaG HEPCGN_01960 0.37 -1.9 320,490 - yiaG HEPCGN_01960 0.37 +0.4 320,490 - yiaG HEPCGN_01960 0.37 -0.2 320,490 - yiaG HEPCGN_01960 0.37 -0.3 320,490 - yiaG HEPCGN_01960 0.37 -0.6 320,746 - HEPCGN_01965 0.16 -0.8 321,022 - HEPCGN_01965 0.87 -0.8 321,062 + -0.3 321,062 + -0.3 321,063 - -1.5 321,072 + -0.9 321,073 - +0.1 321,073 - +0.4 321,229 - +1.8 321,269 + -2.3 321,269 + +1.0 321,278 + +0.7 321,299 + +0.6 321,324 - -1.7 321,466 - rimO HEPCGN_01970 0.13 -0.3 321,618 - rimO HEPCGN_01970 0.24 -0.0 321,668 - rimO HEPCGN_01970 0.28 -0.0 321,668 - rimO HEPCGN_01970 0.28 +0.8
Or see this region's nucleotide sequence