Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_01415 and HEPCGN_01420 are separated by 80 nucleotides HEPCGN_01420 and cydA are separated by 805 nucleotides
HEPCGN_01415: HEPCGN_01415 - hypothetical protein, at 214,081 to 214,503
_01415
HEPCGN_01420: HEPCGN_01420 - hypothetical protein, at 214,584 to 215,528
_01420
HEPCGN_01425: cydA - cytochrome ubiquinol oxidase subunit I, at 216,334 to 217,902
cydA
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 213.586 kb on - strand at 213.697 kb on - strand at 214.348 kb on + strand, within HEPCGN_01415 at 214.348 kb on + strand, within HEPCGN_01415 at 214.349 kb on - strand, within HEPCGN_01415 at 214.373 kb on + strand, within HEPCGN_01415 at 214.455 kb on - strand, within HEPCGN_01415 at 214.758 kb on + strand, within HEPCGN_01420 at 214.796 kb on + strand, within HEPCGN_01420 at 214.890 kb on + strand, within HEPCGN_01420 at 214.890 kb on + strand, within HEPCGN_01420 at 214.935 kb on + strand, within HEPCGN_01420 at 214.935 kb on + strand, within HEPCGN_01420 at 215.079 kb on - strand, within HEPCGN_01420 at 215.182 kb on + strand, within HEPCGN_01420 at 215.219 kb on + strand, within HEPCGN_01420 at 215.334 kb on - strand, within HEPCGN_01420 at 215.453 kb on + strand at 215.475 kb on - strand at 215.528 kb on + strand at 215.529 kb on - strand at 215.529 kb on - strand at 215.616 kb on - strand at 215.669 kb on + strand at 215.669 kb on + strand at 215.781 kb on + strand at 215.810 kb on + strand at 215.821 kb on + strand at 215.891 kb on - strand at 215.970 kb on + strand at 216.146 kb on + strand at 216.151 kb on + strand at 216.155 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 213,586 - -1.4 213,697 - +0.2 214,348 + HEPCGN_01415 0.63 +0.3 214,348 + HEPCGN_01415 0.63 -0.7 214,349 - HEPCGN_01415 0.63 +0.1 214,373 + HEPCGN_01415 0.69 +0.0 214,455 - HEPCGN_01415 0.88 -0.1 214,758 + HEPCGN_01420 0.18 +0.4 214,796 + HEPCGN_01420 0.22 -1.0 214,890 + HEPCGN_01420 0.32 -0.6 214,890 + HEPCGN_01420 0.32 +0.6 214,935 + HEPCGN_01420 0.37 -1.2 214,935 + HEPCGN_01420 0.37 -0.6 215,079 - HEPCGN_01420 0.52 -0.6 215,182 + HEPCGN_01420 0.63 -0.4 215,219 + HEPCGN_01420 0.67 +2.8 215,334 - HEPCGN_01420 0.79 +0.1 215,453 + +1.5 215,475 - -0.2 215,528 + -0.9 215,529 - +0.5 215,529 - -0.0 215,616 - +0.6 215,669 + +1.8 215,669 + +0.7 215,781 + +1.2 215,810 + -0.2 215,821 + -0.9 215,891 - +0.1 215,970 + -2.1 216,146 + +0.3 216,151 + +1.0 216,155 + -2.5
Or see this region's nucleotide sequence