Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hutI and hutG overlap by 1 nucleotides hutG and HEPCGN_01360 are separated by 50 nucleotides
HEPCGN_01350: hutI - imidazolonepropionase, at 198,399 to 199,610
hutI
HEPCGN_01355: hutG - formimidoylglutamase, at 199,610 to 200,560
hutG
HEPCGN_01360: HEPCGN_01360 - histidine ammonia-lyase, at 200,611 to 202,281
_01360
Position (kb)
199
200
201 Strain fitness (log2 ratio)
-2
-1
0
1 at 198.611 kb on + strand, within hutI at 198.625 kb on - strand, within hutI at 198.709 kb on + strand, within hutI at 198.710 kb on - strand, within hutI at 198.713 kb on + strand, within hutI at 198.713 kb on + strand, within hutI at 198.713 kb on + strand, within hutI at 198.714 kb on - strand, within hutI at 198.714 kb on - strand, within hutI at 198.804 kb on - strand, within hutI at 198.888 kb on - strand, within hutI at 198.895 kb on + strand, within hutI at 198.895 kb on + strand, within hutI at 198.895 kb on + strand, within hutI at 198.896 kb on - strand, within hutI at 198.963 kb on + strand, within hutI at 198.964 kb on - strand, within hutI at 198.997 kb on + strand, within hutI at 198.998 kb on - strand, within hutI at 199.066 kb on + strand, within hutI at 199.095 kb on + strand, within hutI at 199.102 kb on + strand, within hutI at 199.103 kb on - strand, within hutI at 199.108 kb on + strand, within hutI at 199.194 kb on + strand, within hutI at 199.267 kb on - strand, within hutI at 199.392 kb on - strand, within hutI at 199.400 kb on + strand, within hutI at 199.448 kb on + strand, within hutI at 199.530 kb on - strand at 199.763 kb on - strand, within hutG at 199.920 kb on + strand, within hutG at 199.921 kb on - strand, within hutG at 199.924 kb on + strand, within hutG at 199.924 kb on + strand, within hutG at 199.947 kb on + strand, within hutG at 200.002 kb on + strand, within hutG at 200.003 kb on - strand, within hutG at 200.003 kb on - strand, within hutG at 200.191 kb on - strand, within hutG at 200.338 kb on - strand, within hutG at 200.485 kb on + strand at 200.741 kb on + strand at 200.846 kb on + strand, within HEPCGN_01360 at 200.868 kb on + strand, within HEPCGN_01360 at 201.017 kb on + strand, within HEPCGN_01360 at 201.027 kb on + strand, within HEPCGN_01360 at 201.028 kb on - strand, within HEPCGN_01360 at 201.030 kb on + strand, within HEPCGN_01360 at 201.031 kb on - strand, within HEPCGN_01360 at 201.062 kb on - strand, within HEPCGN_01360 at 201.062 kb on - strand, within HEPCGN_01360 at 201.076 kb on + strand, within HEPCGN_01360 at 201.087 kb on + strand, within HEPCGN_01360 at 201.087 kb on + strand, within HEPCGN_01360 at 201.088 kb on - strand, within HEPCGN_01360 at 201.103 kb on - strand, within HEPCGN_01360 at 201.201 kb on + strand, within HEPCGN_01360 at 201.250 kb on - strand, within HEPCGN_01360 at 201.276 kb on + strand, within HEPCGN_01360 at 201.277 kb on - strand, within HEPCGN_01360 at 201.316 kb on + strand, within HEPCGN_01360 at 201.488 kb on + strand, within HEPCGN_01360 at 201.500 kb on - strand, within HEPCGN_01360 at 201.510 kb on + strand, within HEPCGN_01360 at 201.510 kb on + strand, within HEPCGN_01360
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 198,611 + hutI HEPCGN_01350 0.17 -0.6 198,625 - hutI HEPCGN_01350 0.19 -0.2 198,709 + hutI HEPCGN_01350 0.26 +0.4 198,710 - hutI HEPCGN_01350 0.26 +0.2 198,713 + hutI HEPCGN_01350 0.26 -0.7 198,713 + hutI HEPCGN_01350 0.26 +0.4 198,713 + hutI HEPCGN_01350 0.26 +0.6 198,714 - hutI HEPCGN_01350 0.26 -0.6 198,714 - hutI HEPCGN_01350 0.26 +0.1 198,804 - hutI HEPCGN_01350 0.33 -1.9 198,888 - hutI HEPCGN_01350 0.40 +1.2 198,895 + hutI HEPCGN_01350 0.41 -0.3 198,895 + hutI HEPCGN_01350 0.41 +0.4 198,895 + hutI HEPCGN_01350 0.41 -0.2 198,896 - hutI HEPCGN_01350 0.41 -2.1 198,963 + hutI HEPCGN_01350 0.47 +0.3 198,964 - hutI HEPCGN_01350 0.47 +0.4 198,997 + hutI HEPCGN_01350 0.49 +0.3 198,998 - hutI HEPCGN_01350 0.49 +0.2 199,066 + hutI HEPCGN_01350 0.55 -1.3 199,095 + hutI HEPCGN_01350 0.57 -0.2 199,102 + hutI HEPCGN_01350 0.58 +0.4 199,103 - hutI HEPCGN_01350 0.58 -0.5 199,108 + hutI HEPCGN_01350 0.58 -0.3 199,194 + hutI HEPCGN_01350 0.66 +0.8 199,267 - hutI HEPCGN_01350 0.72 -0.3 199,392 - hutI HEPCGN_01350 0.82 -0.6 199,400 + hutI HEPCGN_01350 0.83 -0.0 199,448 + hutI HEPCGN_01350 0.87 +0.2 199,530 - -0.3 199,763 - hutG HEPCGN_01355 0.16 -0.8 199,920 + hutG HEPCGN_01355 0.33 -0.1 199,921 - hutG HEPCGN_01355 0.33 -1.3 199,924 + hutG HEPCGN_01355 0.33 +0.4 199,924 + hutG HEPCGN_01355 0.33 -0.6 199,947 + hutG HEPCGN_01355 0.35 +0.9 200,002 + hutG HEPCGN_01355 0.41 -0.6 200,003 - hutG HEPCGN_01355 0.41 +0.1 200,003 - hutG HEPCGN_01355 0.41 -1.6 200,191 - hutG HEPCGN_01355 0.61 -0.5 200,338 - hutG HEPCGN_01355 0.77 +0.3 200,485 + -0.9 200,741 + +0.4 200,846 + HEPCGN_01360 0.14 +0.1 200,868 + HEPCGN_01360 0.15 +1.0 201,017 + HEPCGN_01360 0.24 -0.8 201,027 + HEPCGN_01360 0.25 +0.2 201,028 - HEPCGN_01360 0.25 -0.3 201,030 + HEPCGN_01360 0.25 -1.7 201,031 - HEPCGN_01360 0.25 +0.1 201,062 - HEPCGN_01360 0.27 +0.8 201,062 - HEPCGN_01360 0.27 -0.1 201,076 + HEPCGN_01360 0.28 +1.0 201,087 + HEPCGN_01360 0.28 +0.1 201,087 + HEPCGN_01360 0.28 -1.0 201,088 - HEPCGN_01360 0.29 -0.1 201,103 - HEPCGN_01360 0.29 -1.9 201,201 + HEPCGN_01360 0.35 -1.2 201,250 - HEPCGN_01360 0.38 +0.7 201,276 + HEPCGN_01360 0.40 -1.2 201,277 - HEPCGN_01360 0.40 +0.3 201,316 + HEPCGN_01360 0.42 -0.5 201,488 + HEPCGN_01360 0.52 -0.1 201,500 - HEPCGN_01360 0.53 -0.1 201,510 + HEPCGN_01360 0.54 -0.0 201,510 + HEPCGN_01360 0.54 +1.0
Or see this region's nucleotide sequence